Abstract: 【Objective】 This study was aimed to investigate the effect of high fat and high fructose diet on the morphological changes of retina in elder mice.【Method】 A total of twelve 4-week-old SPF male C57BL/6J mice were fed to 12 months old and then divided into normal diet group (ND) and high fat and high fructose diet group (HFHFD),with 6 mice in each group.The normal diet group was fed with ordinary diet,and the high fat and high fructose diet group was fed with high fat and high fructose diet.The eyeballs were taken at the age of 18 months,the left eyeballs were fixed with eyeball fixation solution,and the right eyeballs were frozen for standby.The structural changes of mouse retina were observed by HE staining,the expression of phosphorylated acetyl CoA carboxylase(p-ACC) was detected by immunohistochemistry (IHC) method,the expression of sterol regulatory element binding protein-1 (SREBP1) was detected by immunofluorescence (IF) method,and the expression of p-ACC,p-AMPK and SREBP1 protein were detected by Western blotting.【Result】 HE staining results showed that compared with normal diet group,the retinal tissues in the high fat and high fructose diet group showed uneven thickness of rods and vertebral layers,local hyperplasia,extension into the outer granular layer,loose arrangement of cells and so on.Immunohistochemistry results showed that compared with normal diet group,the expression of p-ACC in the high fat and high fructose diet group was significantly decreased (P＜0.05).Immunofluorescence results showed that compared with normal diet group,the expression of SREBP1 protein in the high fat and high fructose diet group was significantly increased (P＜0.05).Western bloting results showed that compared with normal diet group,the expression of p-AMPK and p-ACC protein in the high fat and high fructose diet group were significantly decreased (P＜0.05),and SREBP1 protein was significantly increased (P＜0.05).【Conclusion】 Retinopathy in elder mice could be induced by high-fat and high fructose diet,which could inhibit the activation of AMPK,reduce the expression of p-ACC and increase the expression of SREBP1.The results could provide a reference for further exploring the mechanism of high-fat and high fructose diet affecting retinal structural changes.

Key words: high fat and high fructose diet; retinal injury; lipid deposition