C-型利钠肽调控鸡肌内脂肪细胞脂肪沉积的作用机制研究

doi:10.16431/j.cnki.1671-7236.2020.11.017

Abstract

Abstract: The aim of this study was to investigate the regulatory effect and mechanism of C-type natriuretic peptide (CNP) on the proliferation,differentiation and lipolysis of intramuscular preadipocytes (IMPs) in breast muscle of chickens,and lay a foundation on further elucidating the molecular mechanism of intramuscular fat deposition in chickens.The IMPs of breast muscle in Yellow-feathered broilers were used as the model in vitro,the preadipocytes were induced by 10^-7 mol/L CNP.The proliferation,differentiation and lipolysis of IMPs were observed by CCK8,Edu staining,Oil Red O staining and isopropanol extraction,respectively.The concentration of cGMP and glycerin were detected by cGMP and glycerin kit,respectively.The expression of NPRA,NPRB and NPRC genes mRNA was detected by Real-time quantitative PCR.The results showed that compared with control group,the cell proliferation in CNP treatment group was enhanced significantly at 1 and 3 d (P<0.05).The cell differentiation and lipid deposition were extremely significantly decreased at 3 and 6 d (P<0.01),the intracellular cGMP concentration and glycerin concentration in the medium at 3 and 6 d were extremely significantly increased (P<0.01).The expression of NPRB and NPRC genes mRNA in CNP treatment group was extremely significantly or significantly higher than control group (P<0.01;P<0.05),and the expression of NPRA gene mRNA had no significant difference (P>0.05).The results revealed that CNP regulated the lipid deposition of intramuscular adipocytes through NPRB/NPRC-cGMP pathway in chickens.

Key words: C-type natriuretic peptide (CNP); chickens; intramuscular adipocytes; lipid deposition

CLC Number:

S831.2

HUANG Huayun, LIANG Zhong, LIU Longzhou, ZHAO Zhenhua, WANG Qianbao, LI Chunmiao, HUANG Zhenyang, ZHANG Xin, LI Shoufeng. Mechanism of C-type Natriuretic Peptide Regulating Lipid Deposition of Intramuscular Adipocytes in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3555-3562.

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Mechanism of C-type Natriuretic Peptide Regulating Lipid Deposition of Intramuscular Adipocytes in Chickens

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