丁酸梭菌和丁酸钠对高脂饮食诱导雄性小鼠生殖损伤的影响

doi:10.16431/j.cnki.1671-7236.2023.04.032

Abstract

Abstract: 【Objective】 This study was aimed to investigate the effects of Clostridium butyrate (C.butyricum) and sodium butyrate on semen quality and testicular tissue of mice stimulated by high fat diet,so as to provide experimental basis and theoretical support for the treatment of male reproductive diseases.【Method】 In experiment 1,32 8-week-old C57BL/6 male mice were randomly divided into 4 groups.The mice in control group (NC) were fed a basal diet, in high fat diet group (HFD), C. butyricum treatment group (HFD+C.butyricum) and sodium butyrate treatment group (HFD+Sodium butyrate) were fed high fat diet containing 60% fat.HFD+C.butyricum group was gavaged with 1×10⁸ CFU/kg C.butyricum,while HFD+Sodium butyrate group was gavaged with 300 mg/kg sodium butyrate,once a day.In experiment 2,16 8-week-old C57BL/6 male mice were randomly divided into two groups.The normal diet of control group was supplemented with normal saline,and the experiment group was supplemented with C.butyricum solution 1×10⁸CFU/kg by gavage,once a day.After 12 weeks,the animals were sacrificed for sampling.The semen quality was detected by CASA,and the pathological changes of testicular tissue were observed by HE staining.The total antioxidant capacity (T-AOC),the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),the content of malondialdehyde (MDA) in testicular tissue and serum testosterone (T), and the activities of alkaline phosphatase (AKP),acid phosphatase (ACP),lactate dehydrogenase (LDH) and gamma glutamyltransferase (γ-GT) in testicular tissue were detected by corresponding kits.【Result】 In experiment 1,compared with high-fat group,the body weight of mice in C.butyricum treatment group was extremely significantly decreased (P＜0.01),the sperm motility,the number of A+B grade sperm,sperm linear motility and the velocity of average path of mice were significantly increased (P＜0.05),and the straight-line velocity of mice was extremely significantly increased (P＜0.01).The activity of AKP in testicular tissue of mice was significantly increased (P＜0.05).Compared with high-fat group,the body weight of mice in sodium butyrate treatment group was extremely significantly decreased (P＜0.01),the testis index,sperm linear motility,sperm forward motility,straight-line velocity and swing of sperm were significantly increased (P＜0.05),and the sperm motility,the number of A+B grade sperm,the velocity of average path and the activity of γ-GT in testicular tissue were extremely significantly increased (P＜0.01).In addition,sodium butyrate significantly increased the content of serum testosterone and the activity of ACP (P＜0.05),and significantly decreased the content of MDA in testicular tissue of mice (P＜0.05).The pathological section of testicular tissue showed that there was no significant difference in testicular tissue morphology of mice in each group.In experiment 2,compared with control group,the testicular coefficient of mice treated with C.butyricum were extremely significantly increased (P＜0.01),and the level of serum testosterone was significantly increased (P＜0.05),but there were no significant change in semen quality and antioxidant indexes of testicular tissue (P＞0.05).【Conclusion】 C.butyricum and sodium butyrate could significantly increase the content of serum testosterone in mice,improve the antioxidant capacity of testicular tissue,improve the quality and quantity of sperm,protect the structure of testicular tissue,and effectively protect the reproductive system injury caused by obesity in male mice.

Key words: Clostridium butyricum; sodium butyrate; high fat; testis; semen quality

CLC Number:

S816.73

DU Zhaohui, GE Zihao, YOU Junyi, LIU Xiaowei, LIANG Guodong, SUN Shiduo, HU Jianhong, LI Xiao. Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1603-1616.

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Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice

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