丁酸梭菌对高胆碱饮食小鼠血浆游离脂肪酸组成的影响

doi:10.16431/j.cnki.1671-7236.2023.02.010

Abstract

Abstract: 【Objective】 This study was aimed to analyze the effect of Clostridium butyricum on the composition of plasma free fatty acid (FFA) in mice with abnormal lipid metabolism caused by high choline diet,so as to clarify the mechanism of Clostridium butyricum regulating lipid metabolism in high choline diet.【Method】 24 healthy male Kunming mice aged 4 weeks were randomly divided into 3 groups:Normal,model and Clostridium butyricum groups,with 8 mice in each group.After 8 weeks of high choline diet,7 d of administration and 12 h of fasting,the plasma,liver,epididymal fat pad and perirenal fat were collected.Weighing the fat and liver,the blood lipid was detected by biochemical analyzer.HE staining and oil red O staining were used to observe the changes of liver tissue structure and the deposition degree of lipid droplets.The content of trimethylamine oxide (TMAO) in plasma was detected by liquid mass spectrometry (LC-MS) technology.The composition of FFA in plasma was studied by gas chromatography-mass spectrometry (GC-MS) technology and multivariate statistical analysis.【Result】 Compared with model group,the body weight and fat growth in Clostridium butyricum group were effectively inhibited (P＜0.05).After Clostridium butyrate administration,the deposition of liver lipid in mice was decreased. Clostridium butyrate significantly or extremely significantly decreased the contents of plasma triglyceride (TG) and low density lipoprotein (LDL-C) (P＜0.05 or P＜0.01),and significantly increased the content of high density lipoprotein (HDL-C) in mice fed with high choline diet (P＜0.05).After administration of Clostridium butyricum,the plasma TMAO concentration in mice fed with high choline diet was significantly decreased (P＜0.05).In Clostridium butyrate group,the contents of saturated fatty acids (SFA) such as palmitic acid (C16∶0) and stearic acid (C18∶0) were significantly decreased,the contents of palmitoleic acid (C16∶1) and oleic acid (C18∶1 N9C) in monounsaturated fatty acid (MUFA) were significantly decreased,the content of n-3 type polyunsaturated fatty acids (PUFA) was significantly increased,the content of docosahexaenoic acid (DHA) was significantly increased,and the contents of linoleic acid (C18∶2 N6C) and gamma linolenic acid (C18∶3 N6) in n-6 type PUFA were significantly decreased (P＜0.05).In addition, Clostridium butyrate significantly decreased the contents of trans isooleic acid (C18∶1 N9t) and trans linoleic acid (C18∶2 N6t) in plasma (P＜0.05).【Conclusion】 Clostridium butyrate could regulate FFA composition and plasma TMAO content in mice fed with high choline diet,and improve lipid metabolism disorder.

Key words: Clostridium butyricum; high choline diet; free fatty acid (FFA); trimethylamine oxide (TMAO)

CLC Number:

S852.61⁺6.3

CHEN Jing, YU Shuyi, HUANG Hui, LI Jianzhe. Effect of Clostridium butyrate on the Composition of Plasma Free Fatty Acid in Mice Fed with High Choline Diet[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 519-530.

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Effect of Clostridium butyrate on the Composition of Plasma Free Fatty Acid in Mice Fed with High Choline Diet

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