鸡源高产淀粉酶丁酸梭菌的分离鉴定

doi:10.16431/j.cnki.1671-7236.2015.11.036

Abstract

Abstract: In order to obtain a producing amylase Clostridium butyricum,the chicken small intestinal mucous was collected to isolate and screen Clostridium butyricum.First of all,the samples were heated at 80℃ for 10 min for removing most non-spore bacteria,then the samples were inoculated on TSN medium.The suspected Clostridium butyricum strains were screened by colony morphology and microscopic morphology observation.The producing acid ability and amylase activity of the isolates were successively tested,so the C.B1 strain,both producing acid and producing high-yielding amylase,was selected to be identified by physiological and biochemical test and 16S rDNA sequence analysis.The results showed that C.B1 strain had the characteristics of gram-positive anaerobe,rod-shaped and forming a circular or oval spore,16S rDNA sequence was 1450 bp.Homology comparison analysis indicated that it was most closest to Clostridium butyricum with 99% homology.So C.B1 strain was identified as Clostridium butyricum,this study would lay the foundation for developing new microecological preparations.

Key words: Clostridium butyricum; chicken; amylase; isolation; identification

CLC Number:

S852.61⁺6.3

LI Sheng-jie, DING Ke, JIANG Fang-fang, WEI Jia-min, LI Wang, LI Yuan-xiao, SUN Er-gang. Isolation and Identification of Producing High-yielding Amylase Clostridium butyricum from Chicken[J]. , 2015, 42(11): 3073-3079.

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Isolation and Identification of Producing High-yielding Amylase Clostridium butyricum from Chicken

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