家畜免疫去势研究进展

doi:10.16431/j.cnki.1671-7236.2019.06.021

Abstract

Abstract:

Traditional surgical castration technology is being challenged by animal welfare and large-scale standardized livestock production.As an alternative to surgical castration,immuno-castration can block testicular development,control sexual behavior,and avoid the pain caused by surgery.Immuno-castration uses immunological methods to destroy the hormone balance of the hypothalamic-pituitary-gonadal axis,decrease the hormone levels of GnRH,LH,FSH,and gonadal hormone,inhibit the gonadal function,and then achieve the purpose of castration.Immuno-castration includes hormone immuno-castration and gene immuno-castration,and its targets have undergone bottom-up screening on the hypothalamic-pituitary-gonadal axis,which identified are testosterone,LH,FSH,GnRH and KISS-1.The three targets of GnRH hormone,GnRH and KISS-1 genes located upstream on the hypothalamic-pituitary-gonadal axis have better castration effects.Studies on the effect and mechanism of immuno-castration show that immuno-castration has the characteristics of improving production performance,safety and reversibility.While the reversible mechanism of immuno-castration is still unclear.Castration causes the adrenal gland to play a compensatory role,and the effect of immuno-castration on adrenal compensation has not been reported.This paper introduces the immuno-castration mechanism of targets,discusses the characteristics and application of immuno-castration,in order to provide theoretical basis for promoting the application of immuno-castration technology in the field of animal production and reproduction.

Key words: immuno-castration; target; safety; reversibility; adrenal compensation

CLC Number:

S857.12⁺9

ZENG Fanmei, CHONG Yuqing, JIANG Xunping, LIU Guiqiong. Research Progress on Immuno-castration in Livestock[J]. , 2019, 46(6): 1739-1745.

References

[1] HAN Y,LIU G,JIANG X,et al.KISS-1 can be used as a novel target for developing a DNA immunocastration vaccine in ram lambs[J].Vaccine,2015,33(6):777-782.
[2] KHAN M A H,FERRO V A,KOYAMA S,et al.Immunisation of male mice with a plasmid DNA vaccine encoding gonadotrophin releasing hormone (GnRH-Ⅰ) and T-helper epitopes suppresses fertility in vivo[J].Vaccine,2007,25(18):3544-3553.
[3] HARVEY H A,LIPTON A,MAX D T,et al.Medical castration produced by the GnRH analogue leuprolide to treat metastatic breast cancer[J].Journal of Clinical Oncology Official Journal of the American Society of Clinical Oncology,1985,3(8):1068-1072.
[4] MOGER W H,ARMSTRONG D T.Changes in serum testosterone levels following acute LH treatment in immature and mature rats[J].Biology of Reproduction,1974,11(1):1-6.
[5] PETERS J B,SHORT R E,FIRST N L,et al.Ovarian and pituitary gland changes in hysterectomized postpartum sows treated with FSH and oxytocin[J].Journal of Animal Science,1968,27(4):1027-1031.
[6] WAINMAN P,SHIPOUNOFF G C.Effects of castration and testosterone propionate on striated perineal musculature in the rat[J].Endocrinology,1941,29(6):975-978.
[7] 程薇.孕烯醇酮和睾酮主动免疫对公兔生殖性能的影响[D].雅安:四川农业大学,2012. CHENG W.Effects of active immunization with pregnenolone and testosterone on reproductive performance of male rabbits[D].Ya'an:Sichuan Agricultural University,2012.(in Chinese)
[8] SRINATH B R,WICKINGS E J,WITTING C,et al.Active immunization with follicle stimulating hormone for fertility control:A 41/2-year study in male rhesus monkeys[J].Fertility and Sterility,1983,40(1):110-117.
[9] 曹晓涵,韩兴发,杜小刚,等.GnRH主动免疫雌性大鼠调控机制研究[J].畜牧兽医学报,2016,47(3):502-508. CAO X H,HAN X F,DU X G,et al.Regulatory mechanisms of active immunization against gnrh in female rats and its mechanism[J].Journal of Animal Husbandry and Veterinary Medicine,2016,47(3):502-508.(in Chinese)
[10] HAN X,GU L,XIA C,et al.Effect of immunization against GnRH on hypothalamic and testicular function in rams[J].Theriogenology,2015,83(4):642-649.
[11] HAN X,ZHOU Y,ZENG Y,et al.Effects of active immunization against GnRH versus surgical castration on hypothalamic-pituitary function in boars[J].Theriogenology,2017,97:89-97.
[12] LENTS M P,BARBOSA L P,SANTANA A L A,et al.Immunocastration of goats using anti-gonadotrophin releasing hormone vaccine[J].Theriogenology,2018,114:7-13.
[13] FANG F G,YANG Y P,LIU Y,et al.Immunization of male mice with a new recombinant GnRH fusion protein reduces the testicular function[J].Agricultural Sciences in China,2009,8(3):380-385.
[14] KHAN M A,OGITA K,FERRO V A,et al.Immunisation with a plasmid DNA vaccine encoding gonadotrophin releasing hormone (GnRH-Ⅰ) and T-helper epitopes in saline suppresses rodent fertility[J].Vaccine,2008,26(10):1365-1374.
[15] HAN Y,YE W,LIU G,et al.Hepatitis B surface antigen S gene is an effective carrier molecule for developing GnRH DNA immunocastration vaccine in mice[J].Reproduction in Domestic Animals,2016,51(3):445-450.
[16] UENOYAMA Y,NAKAMURA S,HAYAKAWA Y,et al.Lack of pulse and surge modes and glutamatergic stimulation of luteinising hormone release in Kiss1 knockout rats[J].Journal of Neuroendocrinology,2015,27(3):187-197.
[17] CLARKE I J, SMITH J T, MORRISSEY A,et al. Kisspeptin stimulates ovulation in seasonally acylic ewes[A].Proc 89th Annual Meeting of the Endocrine Society[C]. 2007.
[18] DECOURT C,ROBERT V,ANGER K,et al.A synthetic kisspeptin analog that triggers ovulation and advances puberty[J].Scientific Reports,2016,6:26908.
[19] 韩燕国.KISS-1基因免疫去势效果及机制的研究[D].武汉:华中农业大学,2015. HAN Y G.Study on the effect and mechanism of KISS-1 gene immune castration[D].Wuhan:Huazhong Agricultural University,2015.(in Chinese)
[20] ANDRONIE I.Immunocastration in fattening pigs and its effects on productive performance[J].Scientific Papers Animal Science and Biotechnologies,2016,49(1):209-211.
[21] ALUWÉ M,DEGEZELLE I,DEPUYDT L,et al.Immunocastrated male pigs:Effect of 4 vs 6 weeks time post second injection on performance,carcass quality and meat quality[J].Animal,2016,10(9):1466-1473.
[22] LECLERCQ C,PRUNIER A,MERLOT E.Effects of neonatal surgical castration and immunocastration in male pigs on blood T lymphocytes and health markers[J].Animal,2014,8(5):836-843.
[23] THEUBET G,THUN R,HILBE M,et al.Effect of vaccination against-GnRH (Bopriva^®) in the male pubertal calf[J].Schweizer Archiv Für Tierheilkunde,2010,152(10):459-469.
[24] JAGO J G,MATTHEWS L R,TRIGG T E,et al.The effect of immunocastration 7 weeks before slaughter on the behaviour,growth and meat quality of post-pubertal bulls[J].Animal Science,2016,68(1):163-171.
[25] ANDREO N,BRIDI A M,SOARES A L,et al.Fatty acid profile of beef from immunocastrated (BOPRIVA^®) Nellore bulls[J].Meat Science,2016,117:12-17.
[26] NEEDHAM T,LAMBRECHTS H,HOFFMAN L C.The influence of vaccination interval on growth,carcass traits and testicle parameters of immunocastrated ram lambs[J].Small Ruminant Research,2016,145:53-57.
[27] GÖKDAL O,ATAY O,ULKER H,et al.The effects of immunological castration against GnRH with recombinant OL protein (ovalbumin-LHRH-7) on carcass and meat quality characteristics,histological appearance of testes and pituitary gland in Kivircik male lambs[J].Meat Science,2010,86(3):692-698.
[28] HAN Y G,LIU G Q,JIANG X P,et al.Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs[J].Asian-Australasian Journal of Animal Sciences,2018,31(6):835-841.
[29] 宋天增,冯静,鲍宇红,等.去势对雄性岗巴绵羊血液睾酮浓度和生产性能的影响[J].西南农业学报,2015,28(4):1816-1818. SONG T Z,FENG J,BAO Y H,et al.Effects of castration on blood testosterone concentration and growth performance in male Gamba sheep[J].Southwest China Journal of Agricultural Sciences,2015,28(4):1816-1818.(in Chinese)
[30] 王霜.GnRH多肽免疫去势疫苗在大鼠体内的残留及免疫去势动物肉品安全的研究[D].雅安:四川农业大学,2007. WANG S.Study on the residue of GnRH peptide immune castration vaccine in rats and the safety of immunized castration animals[D].Ya'an:Sichuan Agricultural University,2007.(in Chinese)
[31] FREDRIKSEN B,JOHNSEN A M,SKUTERUD E.Consumer attitudes towards castration of piglets and alternatives to surgical castration[J].Research in Veterinary Science,2011,90(2):352-357.
[32] GODFREY S I,WALKDEN-BROWN S W,MARTIN G B,et al.Immunisation of goat bucks against GnRH to prevent seasonal reproductive and agonistic behaviour[J].Animal Reproduction Science,1996,44(1):41-54.
[33] ZAMARATSKAIA G,RYDHMER L,ANDERSSON H K,et al.Long-term effect of vaccination against gonadotropin-releasing hormone,using Improvac^TM,on hormonal profile and behaviour of male pigs[J].Animal Reproduction Science,2008,108(1-2):37-48.
[34] ZERANI M,CATONE G,QUASSINTI L,et al.In vitro effects of gonadotropin-releasing hormone (GnRH) on Leydig cells of adult alpaca (Lama pacos) testis:GnRH receptor immunolocalization,testosterone and prostaglandin synthesis,and cyclooxygenase activities[J].Domestic Animal Endocrinology,2011,40(1):51-59.
[35] SALEHI S,ADESHINA I,CHEN H,et al.Developmental and endocrine regulation of kisspeptin expression in mouse Leydig cells[J].Endocrinology,2015,156(4):1514-1522.
[36] MEI H,DORAN J,KYLE V,et al.Does kisspeptin signaling have a role in the testes[J].Frontiers in Endocrinology,2013,4(8):198.
[37] AKNOUN N,ZATRA Y,KHAMMAR F,et al.Effects of castration and testosterone treatment on adrenal activity of a saharan gerbil,Meriones libycus in breeding and non-breeding season[J].Endocrine,2009,20:1-16.

Research Progress on Immuno-castration in Livestock

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	LI Yuanyuan, YANG Na, TENG Da, HAO Ya, MA Xuanxuan, MAO Ruoyu, WANG Jianhua. Preparation and Evaluation of Efficacy and Safety of Shengtaicin Post-medicated Bath Solution for Dairy Cow [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1617-1631.
[3]	LIU Xinyu, CUI Qiang, ZHANG Lanxin, FAN Quanrong, LI Zhengyi, GAO Ruifeng. Study on Mechanism of Chebulagic Acid in Treatment of Cow Endometritis Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1632-1641.
[4]	CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.
[5]	SUN Panpan, HOU Zhen, HAO Zhili, ZHENG Jiangang, SUN Na, FAN Kuohai, YIN Wei, LI Hongquan. Study on Mechanism of Curcumol Against Encephalomyocarditis Virus Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 341-348.
[6]	FAN Qiuyu, WU Jianwen, LI Huanrong. Network Pharmacology Analysis of Anti-inflammatory Activity of Medicinal Sophora japonica L. [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3622-3632.
[7]	DU Xinze, MA Xinhao, ZHANG Dianqi, DU Jiawei, MA Jing, XIE Kuncheng, HE Jie, ZAN Linsen. Target Gene Prediction and Bioinformatics Analysis of bta-miR-34b/c and bta-miR-449a/b/c [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2451-2461.
[8]	XUE Qian, LI Guohui, XING Weijie, ZHOU Chenghao, ZHANG Huiyong, YIN Jianmei, ZHU Yunfen, CAO Yuxia, SU Yijun, HAN Wei. Screening of lncRNA Related to Inbreeding Depression of Reproduction in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2176-2185.
[9]	HUANG Zhengyang, ZHAO Zhenhua, HUANG Huayun, LI Chunmiao, WANG Qianbao, MU Chunyu, LI Shoufeng. Bioinformatics and Gene Expression Analysis of miR-10b-5p in Different Tissues of Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1262-1270.
[10]	QIAO Chunyu, LIU Jiahe, ZHANG Boxi, HE Yuxi, ZHENG Yuwei, LYU Hongming. Research Progress on Pathogenic Mechanism and Control of Aflatoxin B₁ [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 765-775.
[11]	DENG Zhanzhao, YU Baojun, CAO Guowei, ZHANG Juan, SHI Yonghong, HU Weilon, QIU Xiuling. Target Gene Prediction and Tissue Expression Analysis of let-7a-5p in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3704-3712.
[12]	LU Huan, NIU Xi, HUANG Yali, LI Sheng, RAN Xueqin, HUANG Shihui, WANG Jiafu. Screening of Candidate miRNA for Regulating Xiang Pig Reproduction [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3118-3127.
[13]	CUI Yiyan, DENG Dun, TIAN Zhimei, LU Huijie, LIU Zhichang, LI Jiazhou, MA Xianyong, ZHANG Qi, LAN Yonghui. Research Progress on Nutritional Value and Feed Safety of Food Waste [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2820-2829.
[14]	PAN Chenhao, ZHANG Xin, ZHAO Ruili, JIANG Xuan, JIN Tianming, YU Enyuan, LI Liuan, ZENG Jun, YU Xiaoxue, SONG Qiqi, HU Ye. Construction and Cytotoxicity Detection of Recombinant Epitope Protein DCpep-GSE of Streptococcus suis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2990-3001.
[15]	FU Qiang, YUE Qiaoxian, SONG Pengyan, ZHOU Rongyan. Target Gene Prediction and Bioinformatics Analysis of miR-150 in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2333-2341.