绵羊miR-150靶基因预测及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2021.07.007

Abstract

Abstract: In order to explore the regulatory mechanism of miR-150 in ovaries of sheep,the target gene prediction and analysis was performed with bioinformatic method.Mature miR-150 sequences were obtained from miRBase database for sequence alignment and conservative analysis.The Ensemble database was used to search the miR-150 precursor sequence,and the online software PROMO was used to predict the transcription factor binding site of miR-150.The target genes of intersection were predicted by TargetScan and miRwalk,and the Go function and KEGG pathway enrichment analysis of the predicted target genes were performed by DAVID and KOBAS online software.The functional protein interactions was analyzed with Networkanayst online tools.YM500V2 software was used to analyze the differential expression levels of miR-150 target genes in different tissues and diseases.The results showed that the miR-150 mature sequences between species were highly conservative.There were many transcription factor binding sites such as p53,ID1 and FOXO4 in the promoter region of miR-150.GO function enrichment analysis results showed that the predicted target genes of miR-150 mainly enriched in the positive regulation of RNA polymerase Ⅱ promoter transcription for biological process,zinc ions binding,DNA binding and transcription of DNA template for molecular functions,nuclear,nuclear and cytoplasmic and perinuclear area of cytoplasm for cellular components.KEGG pathway enrichment analysis results showed that target genes were significantly enriched in cancer pathways,proteoglycan in cancer,human papillomavirus infection,breast cancer,regulatory stem cell pluripotency and other signaling pathways.miR-150 target gene interaction analysis results revealed that target genes PIK3R1,PIK3CB and CTNNB1 had more targeted relationships with other proteins,and involved in estrogen signaling pathway,cell senescence,phosphatidylinositol signaling system,TGF-beta and other pathways.The expression of miR-150 in different tissues and diseases was analyzed by YM500V2 online software,it was found that the expression level of miR-150 was the highest in blood,relatively high in ovary,pancreas,lymph and skin,and very low or no expression level in brain and adrenal cortex.These results would provide theoretical reference for investigating the function and regulatory mechanism of miR-150,and further laid the foundation for the regulatory mechanism of miR-150 in the ovarian development of sheep.

Key words: sheep; miR-150; target gene; bioinformatics

CLC Number:

FU Qiang, YUE Qiaoxian, SONG Pengyan, ZHOU Rongyan. Target Gene Prediction and Bioinformatics Analysis of miR-150 in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2333-2341.

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Target Gene Prediction and Bioinformatics Analysis of miR-150 in Sheep

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