基于网络药理学和分子对接探究诃子鞣酸防治奶牛子宫内膜炎的作用机制

doi:10.16431/j.cnki.1671-7236.2023.04.034

Abstract

Abstract: 【Objective】 Based on network pharmacology and molecular docking technology,this paper explored the role of chebulagic acid in preventing and treating cow endometritis,and clarified its potential mechanism.【Method】 The action targets of chebulagic acid were collected and predict through SwissTargetPrediction and PharmMapper databases,and standardized and de-duplicated through UniProt database.Cow endometritis related genes were acquire from OMIM and DRUGBANK databases and de-duplicated.Venn map was drawn to obtain the intersection target of chebulagic acid and cow endometritis.The protein-protein interaction (PPI) network was constructed using STRING database,and the GO function and KEGG pathway enrichment analysis were performed using DAVID database.The molecular docking technology was used to dock the key pathway targets obtained after enrichment analysis of KEGG pathway to verify the accuracy of network pharmacological analysis results.【Result】 333 potential target genes of chebulagic acid and 342 target genes related to cow endometritis were obtained by searching and screening multiple databases,with a total of 18 overlapping targets.PPI results showed that chebulagic acid might act on 18 key targets,such as prostaglandin G/H synthase 1(PTGS1),PTGS2,arachidonate 12-lipoxygenase (ALOX12),ALOX15,etc.. The results of GO founction and KEGG pathway enrichment analysis showed that chebulagic acid could prevent and treat cow endometritis through lipoxygenase pathway,arachidonic acid metabolism pathway,estrogen signal pathway,etc.Molecular docking results showed that chebulagic acid had good connectivity with the key targets obtained,which could play a role in preventing and treating cow endometritis through multiple targets,proving that the network pharmacological prediction results were accurate.【Conclusion】 Chebulagic acid might play a role in preventing and treating cow endometritis by combining with PTGS1,PTGS2,ALOX12 and cellular tumor antigen p53 (TP53) in the lipoxygenase pathway and arachidonic acid metabolism process.

Key words: chebulagic acid; endometritis; network pharmacology; targets; molecular docking

CLC Number:

S859.7

LIU Xinyu, CUI Qiang, ZHANG Lanxin, FAN Quanrong, LI Zhengyi, GAO Ruifeng. Study on Mechanism of Chebulagic Acid in Treatment of Cow Endometritis Based on Network Pharmacology and Molecular Docking[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1632-1641.

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Study on Mechanism of Chebulagic Acid in Treatment of Cow Endometritis Based on Network Pharmacology and Molecular Docking

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