Abstract: 【Objective】 This study was aimed to analyze and determine the pharmacodynamic material basis of anti-inflammatory effect of Phellodendron amurense and Acorus calamus mixture,and determine the possible target.【Method】 The anti-inflammatory effect of Phellodendron amurense and Acorus calamus mixture was tested by auricular swelling test in mice,and then the interaction (PPI) network diagram with inflammation-related proteins was analyzed by network pharmacology.Cytoscape 3.7.2 software was used to draw the network diagram of drug-active component-disease-common target,and GO function and KEGG pathway were enriched and analyzed.Autodock software was used to connect the main active ingredients with the core target. 【Result】 The inhibition rate of auricular swelling induced by xylene in mice by Phellodendron amurense and Acorus calamus mixture was 56.96%,which was significantly different from the model group (P＜0.05).Through network pharmacology analysis,a total of 30 active compounds of Phellodendron amurense and Acorus calamus mixture such as quercetin,berberine,and kaempferol were obtained,and 25 intersection targets of Phellodendron amurense and Acorus calamus mixture and inflammation were interleukin-6 (IL6),IL1β,and serine/threonine protein kinase (AKT1).GO enrichment analysis results showed that a total of 334 biological processes,13 cellular components,and 27 molecular functions were obtained.Biological processes involved positive regulation of nitric oxide biosynthesis process,positive regulation of apoptotic process,positive regulation of smooth muscle cell proliferation,and inflammatory response.Cellular components involved the extracellular space,extracellular region,and secretory granules.Molecular functions involved cytokine activity,enzyme binding,RNA polymeraseⅡtranscription factor activity,and ligand-activated sequence-specific DNA binding.KEGG results analysis yielded 82 pathway,and it was found that there might be some relationship between anti-inflammatory effects of Phellodendron amurense and Acorus calamus mixture and TNF signaling pathway,IL17 signaling pathway,lipids and atherosclerosis.【Conclusion】 Phellodendron amurense and Acorus calamus mixture had a good anti-inflammatory effect,and its anti-inflammatory mechanism might involve the regulation of gene and protein expression of different signaling pathways by multiple active components,indicating that Phellodendron amurense and Acorus calamus mixture exerted anti-inflammatory effects through multiple components,multiple pathways and multiple targets.

Key words: inflammation; Phellodendron amurense; Acorus calamus; mixture; network pharmacology; signaling pathway