乌梅-全蝎双药方对气单胞菌混合感染大口黑鲈的治疗效果分析

doi:10.16431/j.cnki.1671-7236.2023.03.040

Abstract

Abstract: 【Objective】 The aim of this study was to understand and master the pathogenicity of mixed infection of Micropterus salmoides with Aeromonas hydrophila (Ah),Aeromonas sobria (As) and Aeromonas veronii (Av),and screen out effective Chinese medicine for prevention and treatment of Aeromonas infection,so as to provide theoretical basis for prevention and treatment of mixed infection of Aeromonas.【Method】 Ah,As and Av were injected intraperitoneally into healthy Micropterus salmoides to establish the animal model of Micropterus salmoides infected by three Aeromonas alone and co-infected.The pathogenicity of Aeromonas co-infection was analyzed by organ index,histopathological section and mortality rate.The traditional Chinese medicines with inhibitory effect on Aeromonas in vitro was screened from 37 traditional Chinese medicines,and the key active ingredients and action targets of Fructus Mume-Scorpion double prescription in vivo were analyzed by network pharmacology.The therapeutic effect of Fructus Mume-Scorpion double prescription on Micropterus salmoides co-infected with three Aeromonas species was verified by medicinal bath method.【Result】 The pathogenicity of co-infection with three Aeromonas species on Micropterus salmoides was higher than that of infection alone.When infected alone,Ah caused the most severe organ damage,inflammation,and the highest mortality,followed by As and Av.Fructus Mume-Scorpion double prescription had significant inhibitory effect on Aeromonas in vitro,the antibacterial effect of the combination of Fructus Mume-Scorpion double prescription was stronger than that of single use.Through the analysis of network pharmacology,it was found that there were 180 common targets of action of Fructus Mume-Scorpion double prescription and bacterial infection,11 common targets such as tumor protein 53 (TP53),AKT serine/threonine-protein kinase 1 (AKT1) and so on which were potential key targets in the treatment of bacterial infection with Fructus Mume-Scorpion double prescription.The results of medicine bath experiment showed that Fructus Mume-Scorpion double prescription could significantly reduce the clinical symptoms,liver and intestinal inflammation and cell necrosis caused by three Aeromonas species co-infection,and the mortality rate of Micropterus salmoides co-infected with three Aeromonas species was decreased by 66.7%,which had a good therapeutic effect.【Conclusion】 The research indicated that the pathogenicity of Micropterus salmoides co-infection with three Aeromonas species was higher than that of single infection.Fructus Mume-Scorpion double prescription could significantly improve the survival rate of Micropterus salmoides co-infected with three Aeromonas species,the results provided a theoretical basis for the study of pathogenic mechanism and prevention and control measures of Aeromonas co-infection.

Key words: Fructus Mume; scorpion; Micropterus salmoides; Aeromonas; network pharmacology

CLC Number:

S853.74

HOU Tianmu, XIANG Wei, WANG Lingling, GUO Zhongbao, WU Lijun, ZHANG Fuxian. Effect of Fructus Mume-Scorpion Double Prescription on Micropterus salmoides Co-infected with Aeromonas[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1259-1270.

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Effect of Fructus Mume-Scorpion Double Prescription on Micropterus salmoides Co-infected with Aeromonas

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