当归补血汤提取物亚慢性毒性试验研究

doi:10.16431/j.cnki.1671-7236.2017.10.037

Abstract

Abstract:

To evaluate the safety of the Dangguibuxue Tang (DBT) for animals, SD rats were administrated DBT by gavage once daily at 0, 15, 30 and 60 g/(kg·BW) for 28 consecutive days as control, low, medium and high dosage groups, and followed by a 14-day recovery period. The results showed that compared with control group, the weight gain and food intake of male rats in the high dosage group were extremely significantly decreased (P < 0.01). WBC and Lym of male rats in the high dosage group were significantly increased (P < 0.05), whereas ALT, TC and GLU were extremely significantly decreased (P < 0.01),PLT was significantly decreased (P<0.05). RBC, HGB and ALT of female rats in high dosage group were extremely significantly decreased (P < 0.01). ALT and ALP of rats in medium dosage group were significantly or extremely significantly decreased (P < 0.05;P < 0.01). While in medium dosage group RET of male rats was significantly increased (P < 0.05) and HGB of the female rats were significantly decreased (P < 0.05) 14 days after withdrawal. However, these indexes had no significant difference to control group at the end of recovery period. In addition, no changes were observed in the main organs of rats at macroscopic and microscopic level. The result showed that subacute administration of DBT was not toxic in SD rats, suggesting a safety use for animals.

Key words: Dangguibuxue Tang; rat; subacute toxicity

CLC Number:

R285.5

LI Xu-ting, WANG Bin, LI Si-cong, YANG Rui, LI Jin-liang, ZHANG Min. Study on Subacute Toxicity Test of the Extact of Dangguibuxue Tang[J]. , 2017, 44(10): 3090-3099.

References

[1] 曾宇,张三印,胡冠英.当归补血汤的研究进展[J].时珍国医国药,2016,27(2):422-424.
[2] Li X T, Wang B, Li J L, et al. Effects of Dangguibuxue Tang, a Chinese herbal medicine, on growth performance and immune responses in broiler chicks[J]. Biol Res, 2013, 46(2):183-188.
[3] Chen S T, Lee T Y, Tsai T H, et al. The traditional Chinese medicine DangguiBuxue Tang sensitizes colorectal cancer cells to chemoradiotherapy[J]. Molecules, 2016, 21(12):E1677.
[4] Gong A G, Zhang L M, Lam C T, et al. Polysaccharide of Danggui Buxue Tang, an ancient Chinese herbal decoction, induces expression of pro-inflammatory cytokines possibly via activation of NF-κB signaling in cultured RAW 264.7 cells[J]. Phytotherapy Research, 2017, 31(2):274-283.
[5] Gong A G, Lau K M, Zhang L M, et al. Danggui Buxue Tang, Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix, induces production of nitric oxide in endothelial cells:Signaling mediated by phosphorylation of endothelial nitric oxide synthase[J].Planta Medica, 2016, 82(5):418-423.
[6] Xie J H, Chen Z W, Pan Y W, et al. Evaluation of safety of modified-Danggui Buxue Tang in rodents:Immunological, toxicity and hormonal aspects[J]. Journal of Ethnopharmacology, 2015, 183:59.
[7] 李春红, 董玉龙, 史万玉. 当归补血散对高龄蛋鸡生产性能及蛋品质的影响[J]. 中国饲料, 2011, 17:29-30.
[8] 王时光, 杨帆, 梁国庆,等. 当归补血汤对心肌缺血模型大鼠心肌组织氧化应激的影响[J]. 时珍国医国药, 2016,8:1850-1852.
[9] 陈耀辉, 黄丽萍, 吴素芬,等. 当归补血汤与人参当归方、灵芝阿胶方的补气生血作用比较[J]. 中药药理与临床, 2015,1:22-25.
[10] 段文龙, 梁先明. 兽药研究技术指导原则汇编[M]. 北京:化学工业出版社, 2012.
[11] 郜鹏云, 马吉飞. 复方姬松茸多糖口服液对大鼠慢性毒性试验[J]. 中国畜牧兽医, 2014, 41(1):146-149.
[12] 刘宇, 曾豪杰, 尚若锋,等. 黄花补血草总黄酮亚急性毒性试验[J]. 中国畜牧兽医, 2016, 43(8):2135-2142.
[13] 王欣, 王福力, 王颖. 肾上腺糖皮质激素对白细胞总数及分类的影响[J]. 承德医学院学报, 1990, 13(4):290-291.
[14] 刘贵颖, 刘玉庆, 吕英, 等. 中药咳喘方对哮喘豚鼠肺组织病理学影响的研究[J]. 天津中医药, 2008, 25(3):217-219.
[15] 郭静, 艾儒棣, 朱晓燕, 等. 当归饮子对复合型慢性荨麻疹致敏小鼠模型细胞因子的影[J].南京中医药大学学报, 2013, 29(2):159-161.
[16] 蔡萃, 董竞成, 杜文静. 黄芪甲苷对哮喘小鼠嗜酸性粒细胞浸润的影响及机制研究[J]. 中华中医药杂志, 2013, 23(8):2278-2283.
[17] 凌昊, 赵霞. 黄芪多糖治疗哮喘的机制研究进展及展望[J]. 浙江中医药大学学报, 2016, 40(1):72-74.
[18] Xu H D, You C G, Zhang R L, et al. Effects of Astragalus polysaccharides and astragalosides on the phagocytosis of Mycobacterium tuberculosis by macrophages[J]. J Int Med Res, 2007, 35(1):84-90.
[19] Yang X, Zhao Y, Lv Y, et al. Protective effect of polysaccharide fractions from Radix A. sinensis against tert-butylhydroperoxide induced oxidative injury in murine peritoneal macrophages[J]. Journal of Biochemistry & Molecular Biology, 2007, 40(6):928-935.
[20] Yang X, Zhao Y, Wang H, et al. Macrophage activation by an acidic polysaccharide isolated from Angelica sinensis (Oliv) Diels[J]. Journal of Biochemistry & Molecular Biology, 2007, 40(5):636-643.
[21] Yang T, Jia M, Meng J, et al. Immunomodulatory activity of polysaccharide isolated from Angelica sinensis[J]. Int J Biol Macromol,2006, 15, 39(4-5):179-184.
[22] 阴赪宏, 李兰芳, 金亚宏, 等.当归补血汤对小鼠巨噬细胞的活化作用的研究[J].中国中医基础医学杂志,1998,4(7):24-27.
[23] 李春林, 陈思瑞, 彭兵. ITP脾巨噬细胞吞噬功能变化及其与外周血细胞计数变化的关系[J].四川大学学报, 2011, 42(2):277-279.

Study on Subacute Toxicity Test of the Extact of Dangguibuxue Tang

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[2]	LI Can, LI Chuchu, ZENG Wei, ZHANG Ning, JI Chunxiao, CHEN Tao. Recombinant Expression and Function Study of RegⅢγ Protein in Porcine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2414-2426.
[3]	CAO Qi, JIN Qing, YUAN Yi, FENG Yixue, KONG Danni, CHEN Xianghong, WU Bin, SONG Wenbo, TANG Xibiao. Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2439-2449.
[4]	LIU Peng, LI Jingjing, QIAO Yanliang, ZHU Lianqin, ZHU Fenghua. Extraction and Structure Characterization of Scutellaria baicalensis Polysaccharide [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2518-2530.
[5]	NIU Yeyu, SHEN Qi, BAI Yong, SUN Panpan, SUN Na, YIN Wei, FAN Kuohai, LI Hongquan, SUN Yaogui. Anti-inflammatory and Antioxidative Effects of Extracts of Physalis Calyx seu Fructus Based on Ulcerative Colitis Model [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2531-2539.
[6]	PIAN Huifang, DU Xubin, LI Yan, ZHANG Yuchen, LIU Fei, YU Debing. Sequencing Analysis of the Full-length Transcriptome of Granulosa Cells of Small Yellow Follicle in Hy-Line Variety Brown [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1754-1763.
[7]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.
[8]	SONG Chenglei, ZHANG Zhe, WEI Yilin, YANG Songbai, DOU Yaqing, LI Chenlei, LIU Yingke, LI Xinjian, YANG Feng, LI Xiuling, WANG Kejun, QIAO Ruimin, HAN Xuelei. Effects of Different Breeds,Seasons and Month Ages on Semen Quality of Boars [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1938-1946.
[9]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[10]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[11]	ZHAO Qianming, QI Meng, XU Zeli, GONG Pihong, LU Chenyang, PEI Zhiyang, JIAN Fuchun, WANG Jiandong, JING Bo, NING Changsheng. Investigation of Intestinal Parasites Occurrence in Sheep and Environmental Contamination in Large-scale Housing Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2156-2165.
[12]	TANG Yu, YANG Yifeng, ZHANG Ying, XUE Hailong, FENG Huailiang, XU Baozeng. Cytological Study on Oocyte Maturation of Raccoon Dog [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1434-1443.
[13]	WANG Leping, PAN Yan, TAN Yizhou, LI Lei, HAN Kaiou, CAO Yajie, LI Jun, ZHOU Yuqing, LIAO Yuying, MA Dongxin, GONG Yu, PENG Hao, WANG Xiaoye. Isolation,Identification and Genome Analysis of Escherichia coli Phage vB_EcoP_GN07 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1522-1531.
[14]	DU Zhaohui, GE Zihao, YOU Junyi, LIU Xiaowei, LIANG Guodong, SUN Shiduo, HU Jianhong, LI Xiao. Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1603-1616.
[15]	YAO Jiawei, HUANG Yujie, CHEN Zhisheng, WANG Bingyun, ZHANG Hui. Immunomodulatory Effects of Dimethyl Alpha-ketoglutarate Pretreatment on Canine Adipose-derived Mesenchymal Stem Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1642-1652.