鸽GnIH基因多态性及其与产蛋性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.04.013

Abstract

Abstract: 【Objective】 This study was aimed to investigate the polymorphism of the gonadotropin-inhibitory hormone (GnIH) gene in pigeons,and screen the single nucleotide polymorphism (SNP) that significantly affect egg-laying traits in female pigeons for early selection of high-yielding female pigeons.【Method】 A total of 78 pairs of White King pigeons was selected as the research objects,and primers were designed according to the mRNA sequence of GnIH gene in pigeons (GenBank accession No.:MG589639.1) obtained by earlier cloning,and GnIH gene was detected by PCR amplification and direct sequencing for SNP and genotyping.The linkage imbalance analysis was conducted on GnIH gene SNP using Haploview software,and the correlation between the polymorphism of GnIH gene and egg-laying trait in White King pigeons was analyzed by SPSS 22.0 software.【Result】 Seven SNPs were detected in GnIH gene of White King pigeons,which were located in exon 1 (c.59 C＞T and c.72 T＞A),exon 2 (c.398 G＞C) and 3'-UTR (c.768 C＞T,c.860 G＞A,c.909 A＞G and c.961 T＞C),respectively.Expect c.59 C＞T deviated from Hardy-Weinberg equilibrium (P＜0.05),all other SNPs were in Hardy-Weinberg equilibrium (P＞0.05).c.768 C＞T was completely linked to c.961 T＞C (D’=1 and r²=1),c.860 G＞A was strongly linked to c.768 C＞T and c.961 T＞C (D’=1 and r²=0.69),and other SNPs were weakly linked.c.398 G＞C mutation caused changes in amino acid properties and mRNA secondary structure,which was significantly correlated with annual egg production and body weight at the first egg of female pigeons (P＜0.05).【Conclusion】 The SNP on exon 2 of GnIH gene had certain genetic effects on the laying traits of pigeons,which could be used as candidate molecular marker for the egg-laying performance of female pigeons.

Key words: pigeons; GnIH gene; single nucleotide polymorphism (SNP); egg-laying traits; correlation analysis

CLC Number:

S813.3

ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.

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Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons

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