长顺绿壳蛋鸡SLC8A1基因多态性及其对蛋壳品质的影响

doi:10.16431/j.cnki.1671-7236.2022.12.018

Abstract

Abstract: 【Objective】 This study was aimed to explore the effect of solute carrier family 8 member A1 (SLC8A1) gene polymorphism on the eggshell quality in Changshun Blue-eggshell chickens, so as to provide reference for improving eggshell quality in the future.【Method】 The sequence of SLC8A1 gene in chicken was searched by GenBank database (accession No.:NC_052534.1), Primer Premier 3.0 software was used to design primers for PCR amplification, and the SNP of SLC8A1 gene was screened by direct sequencing.The differences of eggshell indexes of different genotypes of SNP were detected by SPSS 22.0 software.【Result】 Three new SNPs were identified in exon 11 of SLC8A1 gene:g.16085681 T>C, g.16085766 C>T and g.16085781 T>C.A total of 3 haplotypes and 6 diplotypes were detected, the haplotypes were H1(TCT), H2(CTC) and H3(TTC), and the diplotypes were H1H1(TTCCTT), H1H2(TCTCTC), H1H3(TTTCTC), H2H2(CCTTCC), H2H3(CTTTCC) and H3H3(TTTTCC).χ² test showed that three SNPs were in Hardy-Weinberg equilibrium (P>0.05), all showed moderate polymorphism.The results of correlation analysis showed that the eggshell strength of TC genotype individual of g.16085681 T>C was significantly higher than that of TT genotype (P<0.05).g.16085681 T>C and haplotype H2 (C_16085681T_16085766C_16085781) had significant effects on the secondary structure of SLC8A1 gene mRNA.The egg shape index of CC genotype individual of g.16085766 C>T was significantly higher than that of TT genotype (P<0.05).The egg shape index of H1H1 and H1H3 individuals were significantly higher than that of H2H3 (P<0.05).The eggshell strength and eggshell thickness of H2H3 individual were significantly higher than those of H1H3 (P<0.05).The egg weight of H1H2 and H2H2 individuals were significantly higher than that of H3H3 (P<0.05).【Conclusion】 g.16085681 T>C could be used a marker site affecting eggshell strength, g.16085766 C>T mutation could be used a marker site affecting egg shape index, and diplotype H2H3 might be a favorable diploid of eggshell strength and eggshell thickness.

Key words: Changshun Blue-eggshell chickens; SLC8A1 gene; polymorphism; eggshell quality; correlation analysis

CLC Number:

S831.2

WANG Yingtong, YANG Suan, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Polymorphism of SLC8A1 Gene and Its Effect on Eggshell Quality in Changshun Blue-eggshell Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4688-4696.

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Polymorphism of SLC8A1 Gene and Its Effect on Eggshell Quality in Changshun Blue-eggshell Chickens

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