China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (1): 107-115.doi: 10.16431/j.cnki.1671-7236.2020.01.013

• Genetics and Breeding • Previous Articles     Next Articles

Polymorphism of STAT3 Gene Exon 16 and Its Association Analysis with Meat Quality and Gene Expression in Jiangkou Luobo Pigs

ZHANG Ji1, WU Xue1, QIU Ganyuan1, LIU Pengcheng1, YANG Maolin2, LIU Ruoyu1   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region(Guizhou University), Ministry of Education, College of Animal Sciences, Guizhou University, Guiyang 550025, China;
    2. Science and Technology Bureau of Jiangkou County, Tongren 554400, China
  • Received:2019-04-15 Online:2020-01-20 Published:2020-01-17

Abstract: In order to study the relationship between signal transducers and activators of transcription 3 (STAT3) gene and meat quality traits in Jiangkou Luobo pigs,11 main meat quality indexes were determined in 105 Jiangkou Luobo pigs,and SNP locus were screened using mixed DNA pool sequencing.Direct sequencing and genotyping of individual samples were carried,the expression of STAT3 gene in longissimus dorsi muscle of individuals with different genotypes was detected.The correlation between SNP and meat quality and gene expression were analyzed by biostatistical software.The results showed that 1 SNP locus (Exon16-C+78T) was detected in exon 16 of STAT3 gene.Only CT and TT genotypes were found by single sample sequencing.Population genetic parameters analysis result showed that T was the dominant allele,and the polymorphism information content showed moderate polymorphism.χ2 test showed that Exon16-C+78T significantly deviated from Hardy-Weinberg equilibrium.The correlation analysis between genotypes and meat quality traits showed that the marbling score and cooked meat percentage of Jiangkou Luobo pigs with TT genotype were significantly higher than that of CT genotype (P<0.05),and the shearing force of TT genotype was significantly lower than that of CT genotype (P<0.05).The expression of STAT3 gene in longissimus dorsi muscle of Jiangkou Luobo pigs with TT genotype was significantly higher than that of CT genotype (P<0.05).The increase of STAT3 gene expression in Jiangkou Luobo pigs might have a positive effect on improving meat quality,TT genotype of STAT3 gene had higher expression level than CT genotype,and the meat quality traits were better.The results indicated that STAT3 gene could be used as a candidate gene for breeding of Jiangkou Luobo pigs,and Exon16-C+78T might be an important SNP locus affecting pork quality traits.

Key words: Jiangkou Luobo pigs; STAT3 gene; SNP; meat quality; gene expression; correlation analysis

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