波尔山羊PLAG1基因克隆、多态性鉴定及其与出生体重、体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.09.020

Abstract

Abstract: 【Objective】 This study was aimed to investigate the sequence characteristics, polymorphisms of plemorphic adenoma gene 1 (PLAG1) gene and its effects on birth weight and body size in goats.【Method】 Boer goats were selected as the object, the PLAG1 gene were cloned and sequenced, the expression pattern of PLAG1 gene in various tissues of Boer goats at different ages and birth weight was identified by Real-time quantitative PCR, the PLAG1 protein level of leg muscle in lambs with different birth weight was detected by Western blotting.The SNPs in CDS and 3'-UTR of PLAG1 gene in Boer goats were further screened, and its correlation between different genotypes and birth weight and body size in Boer goats were analyzed.【Result】 The total length of PLAG1 gene CDS in Boer goats was 1 500 bp, encoding 499 amino acids.PLAG1 protein was relatively conservative.The tissue expression profile showed that the expression of PLAG1 gene in heart, small intestine and leg muscle in lambs with large birth weight were significant or extremely significantly higher than those with small birth weight, the expression of different tissues in fetal Boer goats was significantly or extremely significantly higher than that in adult ewes (P<0.05 or P<0.01). The expression of PLAG1 protein in leg muscles of lambs with large birth weight was extremely significantly higher than those with small birth weight (P<0.01).6 SNPs of PLAG1 gene 3'-UTR in Boer goats were screened, which were 2664 A>G, 2712 A>G, 2721 T>C, 2879 T>A, 2990 A>T and 3270 A>G, respectively.Correlation analysis showed that the birth tube circumference of AG genotype individuals at 2664 A>G was significantly longer than that of GG genotype (P<0.05);The birth length of TT genotype individuals at 2721 T>C was significantly longer than that of TC genotype (P<0.05);The birth tube circumference of TA genotype individuals at 2879 T>A was significantly longer than that of AA genotype (P<0.05);The birth weight of AA genotype individuals at 2990 A>T was significantly higher than that of AT genotype (P<0.05);The birth length of GG genotype individuals at 3270 A>G was significantly longer than that of AA genotype, and the birth chest circumference of AG genotype individuals at 3270 A>G was significantly larger than that of AA genotype (P<0.05).【Conclusion】 The expression of PLAG1 gene in different tissues of fetal goats were higher than those of adult ewes, the expression during early development was related to the birth weight.PLAG1 gene could be used as a molecular marker for early growth and breeding of Boer goats.

Key words: Boer goats; PLAG1 gene; SNPs; birth weight; body size

CLC Number:

S813.3

FENG Xiaopin, LI Yinxia, ZHANG Chenjian, ZHANG Jun, MENG Chunhua, QIAN Yong, CAO Shaoxian. Coning and Polymorphism Identification of PLAG1 Gene and Its Association Analysis with Birth Weight and Body Size Traits in Boer Goats[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3465-3474.

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Coning and Polymorphism Identification of PLAG1 Gene and Its Association Analysis with Birth Weight and Body Size Traits in Boer Goats

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