贵州地方黄牛GHR基因遗传变异、功能预测及其与生长性状的关联性分析

doi:10.16431/j.cnki.1671-7236.2021.03.017

Abstract

Abstract: In order to analyze the polymorphism of GHR gene in cattle,SNPs with significant effects on the growth traits of native cattle in Guizhou were selected.This study used 150 Guizhou local Yellow cattle (Guanling cattle,Sinan cattle,Weining cattle each 50) as the research object,and GHR as the candidate gene,design primers based on the exon sequence of the Yellow cattle GHR gene included in GenBank.The blood of Guizhou local Yellow cattle was collected,blood genomic DNA was extracted and a mixed pool was contructed.The SNPs and typing of GHR gene was verified by PCR amplification sequencing method.Using DNAStar and SPSS 19.0 software to conduct genetic analysis on the SNPs of GHR gene and the correlation analysis with the growth traits in Guizhou local Yellow cattle,looking for the difference in the growth performance of Guizhou local Yellow cattle with different genotypes.At the same time,bioinformatics was used to analyze the changes of mRNA secondary structure before and after GHR mutation,predictive analysis of the structure and function of GHR protein in Guizhou local Yellow cattle.The results showed that a total of 8 SNPs were detected of GHR gene in Guizhou local Yellow cattle,namely Exon9-C162T,Exon9-C201T,Exon9-G243C,Exon9-G383A,Exon9-A495T,Exon9-C622T,Exon9-C642T and Exon9-A650C.Among them,Sinan cattle had no Exon9-G243C.Analysis of genetic characteristics showed that except for Exon9-G243C locus deviated from Hardy-Weinberg equilibrium in Weining cattle,the remaining SNPs did not deviate from Hardy-Weinberg equilibrium.Correlation analysis showed that GHR gene mutation sites were not detected in Guanling cattle and Sinan cattle.However,in Weining cattle,the chest circumference of individuals with Exon9-G243C GC genotype was significantly lower than that of GG and CC genotypes (P<0.05),the obliquee body length and ischial width of individuals with Exon9-A650C AA genotype were significantly higher than those with CC genotype (P<0.05),while the chest depth was significantly lower than that of AC and CC genotypes (P<0.05).The remaining 6 SNPs were not significant (P>0.05).Bioinformatics analysis showed that before and after the mutation,except for Exon9-G383A mRNA secondary structure did not change,the other SNPs mRNA secondary structure changed,Exon9-G243C and Exon9-A650C would affect the changes of protein secondary structure,but the mutation did not affect the change of the tertiary structure of the protein.In addition,Guizhou local Yellow cattle GHR protein was a secreted protein with signal peptide cleavage sites and 6 N-glycosylation sites.It showed that GHR gene had a high degree of variation.The results showed that the two SNPs of GHR gene Exon9-G243C and Exon9-A650C had significant effect on the growth traits of Guizhou local Yellow cattle,and might be used as candidate molecular markers for the growth and development of Guizhou local Yellow cattle.

Key words: Guizhou local Yellow cattle; GHR gene; SNPs; growth traits

CLC Number:

S827

ZHOU Zhinan, WU Yu, CHEN Xiang, SONG Rumou, CHEN Wei. Genetic Variation and Function Prediction of GHR Gene and Correlation Analysis with Growth Traits in Guizhou Local Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 932-945.

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Genetic Variation and Function Prediction of GHR Gene and Correlation Analysis with Growth Traits in Guizhou Local Yellow Cattle

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