野猪、松辽黑母猪及其杂交一代多性状功能基因分子遗传标记研究

doi:10.16431/j.cnki.1671-7236.2021.03.018

Abstract

Abstract: The purpose of this study was to understand the genetic variation of important economic traits and functional genes in experimental pig population using molecular breeding gene chip of "Zhongxin No.1",so as to provide effective information for selecting excellent breeding individuals.In this study,the effective mutation sites from the functional genes were selected as molecular genetic breeding markers.Those functional genes control the fat deposition,meat quality,growth,disease resistance and coat color phenotype of pigs.A total of 106 individuals inclding wild boars,Songliao Black sows and their hybrids generation were used as experimental animal model.The effective mutation sites on functional genes controlling different traits from experimental animals were statistically analyzed by pig molecular breeding chip "Zhongxin No.1".The results showed that the effective mutation sites for the functional genes controlling fat deposition traits (SCD,MYH4) in pigs were favorable genotypes (CC,TT) for intramuscular fat deposition.The effective mutation sites for the functional genes controlling meat quality trait (PHKG1,PRKAG3,RYR1) were the favorable genotypes (CC,GG,CC) except 21 pigs carrying allele T (heterozygous genotype CT) with effective mutation site of RYR1 functional gene which was unfavorable to meat quality traits.The proportion of homozygous genotype (GG) for disease-resistant favorable allele at the effective mutation site of disease-resistant trait functional gene MUC13 was higher than that of heterozygous (GA) and unfavorable allele homozygous genotype (AA).The detection of effective mutation sites for growth trait functional genes (HMGA1,VRTN,CCKAR) found that there was only one heterozygote,without individuals having TT genotype with the HMGA1 mutation site for body length increase in pigs.The frequency of VRTN effective mutation site T allele controlling rib number was high,which controlled the increase of the number of individual thoracic vertebrae.The effective mutation site for functional gene CCKAR were all C allele homozygous genotype that were beneficial to increase feed intake and daily weight gain.The effective mutation site for KIT functional gene controlling coat color phenotype traits were GG genotype in all tested animals,indicating that there were no gene mutation sites affecting white coat phenotype in the studied pigs.The above results were consistent the coat phenotype of experimental pigs.These results would provide useful information for pig breeding schemes.

Key words: pigs; phenotype traits; functional genes; molecular genetic marker; marker assisted selection

CLC Number:

S813.3

YANG Guangli, ZHANG Huan, TIAN Huiyue, LIU Zhiqiang, ZHANG Zhiyan, SUN Jiafu, FENG Shaoyin, ZHANG Shumin, LI Ming. Study on Molecular Genetic Marker of Multi-traits Functional Genes in Wild Boars,Songliao Black Sows and Their Hybrid First Generation[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 946-953.

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Study on Molecular Genetic Marker of Multi-traits Functional Genes in Wild Boars,Songliao Black Sows and Their Hybrid First Generation

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