关岭牛TBC1D7基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.08.018

Abstract

Abstract: In order to investigate the effect of single nucleotide polymorphism (SNP) of TBC1 domain family member 7(TBC1D7) gene on growth traits in Guanling cattle, in this study, blood samples were collected from 116 Guizhou Guanling cattle, and 8 growth traits were determined.All exons of TBC1D gene were amplified by mixed DNA pool, SNP was screened by direct sequencing, and then amplified and sequenced with individual sample DNA as template to verify SNP and used for genotyping.The correlation between different genotypes of TBC1D gene and the growth traits of Guanling cattle was analyzed by single factor variance of SPSS 19.0 software.The results showed that g.12972 T>C, g.12984 A>G, g.20538 C>T, g.20577 T>C and g.24807 G>A mutation sites were found in exons 5, 6 and 8 of TBC1D gene.The results of genotyping showed that there were 3 genotypes in all 5 SNPs.Chi-square (χ²) test showed that 5 SNPs were significantly deviated from Hardy-Weinberg equilibrium state (P<0.05).The analysis of population genetic parameters showed that 5 SNPs had high heterozygosity and moderately polymorphic information content (0.25<PIC<0.5).The correlation analysis results showed that the oblique length, chest circumference and rear hip circumference of GA genotype individuals in g.24807 G>A of TBC1D gene were significantly higher than that of AA genotype individuals (P<0.05).There were 6 haplotypes(H1-H6) and 9 diplotypes(H1H1, H2H2, H2H3, H2H6, H3H3, H3H4, H3H5, H3H6 and H5H5) of 5 SNPs in the experimental population, including dominant haplotypes H3 and H2, dominant diplotypes H3H3, H2H2 and H2H3.The results of diplotype association analysis showed that H3H6 and H5H5 individuals had significant advantages in body length and hip circumference compared with other diplotypes, and H3H6 and H5H5 might be the favorable genotypes.This results showed that g.24807 G>A of TBC1D gene was an important SNP affecting the growth and development of Guanling cattle, which provided a theoretical reference for screening molecular markers that were helpful to the growth and development of Guanling cattle.

Key words: Guanling cattle; TBC1D7 gene; SNPs; growth trait; correlation

CLC Number:

S813.3

LIU Pengcheng, ZHANG Ji, QIU Ganyuan, GONG Yu, LI Xuesong, LI Wei, ZHANG Yiyu, LIU Ruoyu. Polymorphism of Bovine TBC1D Gene and Its Association with Growth Traits in Guanling Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2853-2863.

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Polymorphism of Bovine TBC1D Gene and Its Association with Growth Traits in Guanling Cattle

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