发情和乏情初产母猪下丘脑-垂体-卵巢轴差异表达基因筛选及分析

doi:10.16431/j.cnki.1671-7236.2021.08.019

Abstract

Abstract: In this study, gene expression differences in hypothalamus-pituitary-ovary axis of estrous and anestrous primiparous sows was analyzed and molecular mechanisms for the regulation of sow estrus was explored.6 healthy primiparous sows were divided into 2 groups:The estrous group (3 sows) and anestrous group (3 sows), and then all sows were slaughtered to collect their hypothalamuses, pituitaries and ovaries for RNA-Seq analysis, GO classification, KEGG enrichment analysis and Western blotting analysis of related protein.It was found that 52 432 800, 52 573 730 and 52 209 252 clean reads were obtained from hypothalamuses, pituitaries and ovaries of estrous sows, respectively.And the ratio of these reads to porcine reference genome (Sus scrofa 11.1) were 78.69%, 81.49% and 79.26%, respectively.52 516 724, 52 476 820 and 52 195 962 clean reads were obtained from hypothalamuses, pituitaries and ovaries of anestrous sows, respectively.And the ratio of these reads to porcine reference genome (Sus scrofa 11.1) were 82.38%, 83.05% and 80.20%, respectively.Compared with estrous sows, there were 710 differentially expressed genes in the hypothalamus of anestrous sows, including 392 up-regulated and 318 down-regulated genes.There were 707 differentially expressed genes in the pituitary of anestrous sows, including 283 up-regulated and 424 down-regulated genes.There were 956 differentially expressed genes in the ovary of anestrous sows, including 635 up-regulated and 321 down-regulated genes.36 differentially expressed genes were shared in 3 kinds of tissues.This study found KISS1, GPR54, TAC3, TACR3, CYP17A1, CYP19A1, STAR, GnRHR and ESR1 genes were all significantly down-regulated in anestrous sow (P<0.05;P<0.01), which were related to regulation of sow estrus.Western blotting analysis indicated TAC3, TAC3R, KISS1 and GPR54 proteins were also all significantly down-regulated in anestrous sow.Analysis of GO and KEGG pathway revealed the differently expressed genes were significantly enriched in some signaling pathways related to steroid hormone production and follicular development, such as positive regulation cholesterol esterification, ovarian steriodogenesis, GnRH signaling pathway and FoxO signaling pathway.This study showed that genes related to the regulation of sow estrus were differentially expressed in hypothalamus-pituitary-ovary axis of estrous and anestrous primiparous sows and especially the mRNA and protein expression levels of Kisspeptin/GPR54 and TAC3/TACR3 systems were all significantly down-regulated in hypothalamus of anestrous primiparous sows.It was speculated that impaired expression of Kisspeptin/GPR54 and TAC3/TACR3 systems led to hypothalamic dysfunction, and in turn led to anestrus in primiparous sows.This study provided important support for revealing the molecular regulation mechanism of anestrus in primiparous sows and a important theoretical basis for the use of molecular technology to improve sow anestrus in the future.

Key words: estrus; anestrus; primiparous sows; hypothalamus-pituitary-ovary axis; mRNA

CLC Number:

S828

REN Qiaoling, ZHANG Jiaqing, GUO Hongxia, WANG Xianwei, WANG Jing, LYU Lingyan, CHEN Junfeng, MA Qiang, ZHANG Bin, XING Baosong, XU Zejun. Screening and Analysis of Differentially Expressed Genes in Hypothalamus-Pituitary-Ovary Axis of Estrous and Anestrous Primiparous Sows[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2864-2877.

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Screening and Analysis of Differentially Expressed Genes in Hypothalamus-Pituitary-Ovary Axis of Estrous and Anestrous Primiparous Sows

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