哈萨克马和焉耆马不同组织中IGFBP-5基因表达差异研究

doi:10.16431/j.cnki.1671-7236.2016.09.006

Abstract

Abstract:

The study aimed to explore the mRNA expression pattern of insulin-like growth factor binding protein-5 (IGFBP-5) gene in different tissues of Kazakh and Yanqi horses.The expression of IGFBP-5 gene in different tissues of heart,liver,spleen,lung,kidney,small intestine,large intestine,cecum,intercostal muscles,longissimus dorsi muscles,brachialis muscle and gluteus in two horses were detected by Real-time quantitative PCR and compared the mRNA expression in the same tissues of two breeds.The results showed that the expression of IGFBP-5 in longissimus dorsi muscle and brachialis muscle of two breeds were significantly higher than other tissues including heart,liver,spleen,lung,kidney,small intestine,large intestine and cecum (P<0.05),and was the lowest in large intestine.The expression of IGFBP-5 in kidney,small intestine,large intestine,cecum,longissimus dorsi muscle and brachialis muscles of Yanqi horse were higher than in the same part of Kazakh horse,and among those in longissimus dorsi muscle and large intestine of Yanqi horse were extremely significantly higher than in the same part of Kazakh horse (P<0.01),and in small intestine and cecum of Yanqi horse were significantly higher than in the same part of Kazakh horse (P<0.05).The test was for further researching the biological function of IGFBP-5 gene,and it could provide a theoretical basis for genetic improvement of production performance of horse in our country.

Key words: IGFBP-5 gene; Kazakh horse; Yanqi horse; mRNA expression; Real-time quantitative PCR

CLC Number:

Q786

TUERXUNJIANG·Wumueraili, MENG Jun, WANG Jian-wen, ZENG Ya-qi, YAO Xin-kui, LI Lin-ling, YAO Fang-fang, WANG Huan, REN Hai-fan, HUANG Jing-jing, A Mi-na. Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses[J]. , 2016, 43(9): 2257-2264.

References

[1] Lochrie J D,Phillips K,Tonner E,et al.Insulin-like growth factor binding protein (IGFBP)-5 is upregulated during both differentiation and apoptosis in primary cultures of mouse mammary epithelial cells[J].Journal of Cellular Physiology,2006,207(2):471-479.
[2] Tripathi G,Salih D A M,Drozd A C,et al.IGF-independent effects of insulin-like growth factor binding protein-5(IGFBP-5) in vivo[J].The FASEB Journal,2009,23(8):2616-2626.
[3] 高雪,许尚忠,张小辉,等.南阳牛肌肉发育过程中IGF结合蛋白的表达变化研究[J].云南农业大学学报(自然科学),2012,4:515-520.
[4] 努尔江·买迪尔,王建文,姚新奎,等.哈萨克马主要体尺指标与屠宰指标相关性研究[J].新疆农业科学,2013,50(8):1549-1553.
[5] 国家畜禽遗传资源委员会(组编).中国畜禽遗传资源志-马驴驼志[M].北京:中国农业出版社,2010.
[6] Kennneth J,Llvak,Thomas D,et al.Analysis of relative gene expression data using Real-time quantitative PCR and the 2^-ΔΔCt method[J].Genome Res,2001,25(4):402-408.
[7] Choudhary V,Kumar P,Bhattacharya T K,et al.DNA polymorphism of insulin-like growth factor binding protein-3 gene and its association with birth weight and body weight in cattle[J].Journal of Animal Breeding & Genetics,2007,124(1):29-31.
[8] James P L,Jones S B,Busby W H J,et al.A highly conserved insulin-like growth factor binding protein (IGFBP-5) is expressed during myoblast differentiation[J].J Biol Chem,1993,268(30):22305-22312.
[9] Stattin P,Bylund A,Rinaldi S,et al.Plasma insulin like growth factor-I,insulin-like growth factor binding proteins,and prostate cancer risk:A prospective study[J].JNCI Cancer Spectrum,2000,92:1910-1917.
[10] Kou K,James P L,Clemmons D R,et al.Identification of two clusters of mouse insulin like growth factor binding protein genes on chromosomes 1 and 11[J]. Genomics,1994,21:653-655.
[11] Wang X J,Su H M,Liang Y,et al.Stable transfection and identification of a hair follicle specific expression vector of IGFBP-5 in goat fetal fibroblasts[J].Genetics and Molecular Research,2014,13(1):1885-1892.
[12] 毛胜.IGF轴的重要成员——IGFBP-5的结构与功能的研究进展[J].职大学报,2009,4:87-91.
[13] Cobb L,Salih D,Gonzalez I,et al.Partitioning of IGFBP-5 actions in myogenesis:IGF-independent anti apoptotic function[J].Journal of Cell Science,2004,117:1737-1746.
[14] Clemmons D R.Use of mutagenesis to probe IGF binding protein structure/function relationships[J].Endocr Rev,2001,22:800-817.
[15] Berfield A K,Andress D L,Abrass C K. IGFBP-5 stimulates Cdc42GAP Agg regation and filopodia formationin migrating mesangial cells[J].Kidney Int,2000,57:1991-2003.
[16] Kuemmerle J F,Zhou H.Insulin like growth factor binding protein-5(IGFBP-5) stimulates growth and IGF-Ⅰ secretion in human intestinal smooth muscle by Ras dependent activation of p38 MAP kinase and Erk1/2 pathways[J].J Biol Chem,2002,277:20563-20571.
[17] 张国梁,吴健,李旭.IGFBP-5基因在草原红牛阉割牛与公牛不同组织中的表达差异[A].中国畜牧兽医学会养牛学分会2011年学术研讨会论文集[C].2011.
[18] 顾以韧,张凯,李明洲,等.猪背最长肌中胰岛素样生长因子(IGFs)系统基因的发育表达模式[J].遗传,2009,31:537-543.
[19] 吴苏日古嘎.IGFBP-5基因在蒙古马不同组织及不同运动时期的表达量研究[D].呼和浩特:内蒙古农业大学,2013.
[20] 吴庆燕,贾私瑶,石惠,等.IGFBP-5基因在不同猪种间的表达差异及5'端序列特征分析[J].中国兽医学报,2015,35(4):655-660.
[21] Rejduch B,Oczkowicz M,Piestrzyńska-Kajtoch A,et al.Expression of IGFBP-3 and IGFBP-5 genes in muscles of pigs representing five different breeds[J].Journal of Animal and Feed Sciences,2010,19:554-563.

Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[2]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[3]	MIAO Manning, GUO Yiwen, HU Debao, ZENG Yuhan, LI Xin, ZHANG Linlin, DING Xiangbin, GUO Hong. Transcriptome Sequencing and Bioinformatics Analysis of MSTN Gene-edited Bovine Muscles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2271-2281.
[4]	SHU Jingting, JI Gaige, TU Yunjie, ZHANG Ming, JU Xiaojun, SHAN Yanju, LIU Yifan, ZOU Jianmin. Tissue Expression Analysis of Wnt3a Gene and Genetic Effects of SNPs on Feather Follicle Density in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1672-1680.
[5]	LI Heng, ZI Xiangdong. Cloning and Expression in Cumulus-oocyte Complexes of Yak TEDDM1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1284-1293.
[6]	CHEN Lei, YUAN Feng, HE Sangang, MAYILA, LI Wenrong. Cloning,Sequencing and Quantitative Expression of FGF10 Gene in Sheep During Hair Follicle Development Period [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 425-432.
[7]	DAI Zhenglie, ZHU Jingjing, CHEN Zhenyu, ZHOU Xiaolong, WANG Han, LI Xiangchen, ZHAO Ayong, YANG Songbai. Cloning, Bioinformatics Analysis and Expression of Porcine SENP1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4382-4390.
[8]	JIANG Yixuan, WANG Yaling, XING Shanshan, SONG Guohua, XU Huifen, LI Ming. Cloning, Bioinformatics Analysis and Expression Profile Construction of MSTN Gene in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3501-3511.
[9]	LI Zhiqiang, TAO Chenyu, WEI Aolong, JIA Qing. Establish on Real-time Quantitative PCR Method for Purity Detection of Porcine Sex-controlled Sperm [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3682-3690.
[10]	WANG Zhengfan, ZHU Lisai, WANG Juan, LI Yiyun, XIANG Rui, YING Biyun, WANG Guiping, JIA Aiqing, BAI Aiquan. Establishment and Preliminary Application of Duplex Real-time Quantitative PCR for Detection of PDCoV and TGEV [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3855-3863.
[11]	LI Anqi, JIANG Lei, SU Xiaotong, ZAN Linsen, WANG Hongbao. Expression Characteristics Analysis of ACTC1 Gene in Qinchuan Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2359-2367.
[12]	LI Jinghui, XING Siyuan, WANG Xicai, LI Qinghe, ZHAO Guiping, ZHANG Yonghong, WEN Jie, LIU Ranran. Screening of Candidate Genes Related to Intramuscular Fat Deposition in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1828-1836.
[13]	WANG Zhenmei, DUAN Mengqi, LU Fenghui, WANG Shixin, QIANGJIUZHUOMA, TAN Zhankun, ZHANG Jian, SHANG Peng. Polymorphism and Tissue Expression Patterns of CKMT2 Gene in Tibetan and Yorkshire Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3305-3313.
[14]	ZHANG Ji, YI Ming, CHENG Chaoyou, CHENG Junhua, FU Zhengxian, YANG Dewen, WU Xue, QIU Ganyuan, LIU Ruoyu. Study on Tissue Expression of SOCS7 and GFAP Genes in Weining Sheep [J]. , 2019, 46(7): 1899-1906.
[15]	ZHAO Chengxiang, DAI Kaiyu, DU Yulu, BAO Wenbin, WU Shenglong. Analysis of the Relationship Between Expression Level of Porcine TLR5 Gene and Infection of Escherichia coli F18 [J]. , 2018, 45(5): 1163-1169.