感染鲤浮肿病毒镜鲤的组织病理变化及病毒分布规律研究

doi:10.16431/j.cnki.1671-7236.2022.03.030

Abstract

Abstract: 【Objective】 This study was aimed to study the histopathological characteristics of mirror carps infected with Carp edema virus (CEV) and the distribution of CEV in various tissues, in order to provide references for the diagnosis, prevention and control of Carp edema virus disease (CEVD).【Method】 The pathological changes of heart, liver, skin, spleen, kidney, brain and gill tissues of infected mirror carps were observed by HE staining.The immunohistochemistry was used to detect the distribution and protein expression of CEV in the tissues of mirror carps.Three probes were designed in the conserved region of CEV P4a gene and labeled at the 5'end FAM for in situ hybridization to detect the nucleic acid distribution of CEV in the tissues of mirror carps.Real-time quantitative PCR was used to detect the viral load in each tissue of diseased mirror carps.【Result】 HE staining analysis showed that the gills of the diseased mirror carps were swollen and hyperemic, and there were red blood cells and inflammatory cells between the gills.The liver cells were deeply stained and atrophied, and the bile duct was bleeding.There was obvious space and hemorrhage in spleen tissue.The renal tissue hyperemia was obvious and the renal tubules were obviously closed.The results of immunohistochemistry showed that the CEV P4a protein was highly expressed in gill and liver tissues of the diseased fish, and less expressed in other tissues.The results of in situ hybridization showed that CEV nucleic acids were abundant in gill tissue, and a small amount of positive signals were found in heart, spleen and kidney.Real-time quantitative PCR assay indicated that the viral load of gill was extremly significantly higher than that in myocardium, liver, skin, spleen, kidney and brain tissues (P<0.01).【Conclusion】 After infection with CEV, visceral tissues of mirror carps showed varying degrees of congestion and bleeding, the virus was mainly distributed in gill, indicating that gill was the main target organ for CEV proliferation.

Key words: Carp edema virus; HE staining; immunohistochemistry; in situ hybridization; Real-time quantitative PCR

CLC Number:

S943

LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.

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Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus

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