不同日龄滩羊皮肤组织化学特征比较

doi:10.16431/j.cnki.1671-7236.2021.08.009

Abstract

Abstract: This study aimed to investigate developmental characteristics of hair follicles and the distribution of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (VEGFR2) in 2-day-old and 35-day-old Tan sheep's skin.The cytochemical characteristics of the skin were observed under the microscopy by HE, modified Masson, Gomori and PATH staining, while the expression of VEGF and VEGFR2 were investigated by histochemical analyses using immunohistochemical and immunofluorescence, IPP image analysis software was used for quantitative analysis.The results showed that compared with 2-day-old Tan sheep, the hair follicle of 35 days was developed well, the boundary between epidermis and dermis was clearer, and the collagen and elastic fibers increased and forming a grid-like distribution, and the density of hair follicles was decreased (P<0.05).The expression of VEGF and VEGFR2 were strong in epidermis, outer root sheath and sebaceous glands of the skin.The average optical of VEGF and VEGFR2 was higher in 2-day-old than in 35-day-old Tan sheep skin tissues(P<0.05).Therefore, the collagen and elastic fibers increased obviously during the development of Tan sheep hair follicle;The pathway of VEGF and VEGFR2 played a direct regulatory role in hair follicle keratinization.

Key words: Tan sheep; hair follicle; vascular endothelial growth factor (VEGF); vascular endothelial growth factor receptor 2 (VEGFR2); immunohistochemistry

CLC Number:

S826

GUO Yan, DING Wei, TAO Jinzhong, CHEN Shaoyu, YUAN Ligang, LI Yingkang. Comparison of Skin Histochemical Characteristics in Tan Sheep at Different Ages[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2762-2770.

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Comparison of Skin Histochemical Characteristics in Tan Sheep at Different Ages

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