舍饲、放牧对滩羊股二头肌纤维特性及宰后成熟过程中蛋白降解的影响

doi:10.16431/j.cnki.1671-7236.2021.08.013

Abstract

Abstract: This study was aimed to investigate the effects of concentrates (CF) and pasture feeding (PF) on meat quality, muscle fiber characteristics, microstructure and protein degradation during postmortem aging of biceps femoris in Tan sheep.28 male Tan lambs aged 4 to 5 months were randomly divided into two groups with 14 replicates each.A replication consisted of 1 sheep.Each group was raised for 60 days under CF and PF, respectively.The results showed that the shearing force, hardness and cohesiveness of muttons in PF group were significantly higher than that in CF group (P<0.05), while the springiness of muttons in PF group was significantly lower than that in CF group (P<0.05).The muscle fiber density and quantity proportion of type Ⅱ muscle fiber of muttons in PF group were significantly lower than that in CF group (P<0.05).Meanwhile, the diameter, cross-sectional area and quantity proportion of type Ⅰ muscle fibers in PF group were significantly higher than that in CF group (P<0.05).During postmortem aging, the sarcoplasmic protein solubility in CF group was significantly higher than that in PF group (P<0.05).The myofibrillar protein solubility in two groups gradually increased after reaching the lowest at 24 h postmortem, but there was no significant difference in each time pointing (P>0.05).Myofibrillar fragmentation index significantly increased at 50.97% and 41.94% in CF and PF groups, respectively.The microstructure showed that the myofibril structure of postmortem muscle was loose and cracked, Z-line was degraded and myofibril fragmentation appeared.The damage of myofibril structure in CF group was more serious than that in PF group.The sarcoplasmic protein had less degeneration and slow degradation rate in CF group, while the degradation of myofibrillar protein was more obvious, and the amount of small molecular protein in CF group was higher than that in PF group.In conclusion, the feeding system affected the characteristics of the biceps femoris muscle fibers in Tan sheep, and had an impact on the microstructure and protein degradation during postmortem aging.CF increased the density and reduced the diameter and the cross-sectional area of muscle fibers of biceps femoris, and thus reduced the muscle shearing force.In addition, as the proportion of type Ⅱ muscle fibers increased, CF improved the growth rate of myofibril fragmentation index, sarcoplasmic protein solubility and protein degradation rate postmortem, it accelerated the process of postmortem aging and improved the tenderness of biceps femoris.

Key words: Tan sheep; concentrates feeding; pasture feeding; biceps femoris; muscle fiber characteristics; postmortem aging

CLC Number:

S826.5

YANG Yuanyuan, HAN Yunsheng, LI Jing, JIA Xueting, ZHAO Qingyu, TANG Chaohua, MA Qing, MA Lina, ZHANG Junmin. Effects of Concentrates and Pasture Feeding on Muscle Fiber Characteristics of Biceps Femoris in Tan Sheep and Its Protein Degradation During Postmortem Aging[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2797-2808.

References

[1] 岳彩娟,梁小军,王秀琴,等.滩羊和湖羊背最长肌全基因组甲基化差异分析[J].中国畜牧兽医,2020,47(10):3058-3068. YUE C J,LIANG X J,WANG X Q,et al.Whole genome methylation variation analysis of longissimus dorsi between Tan sheep and Hu sheep[J].China Animal Husbandry & Veterinary Medicine,2020,47(10):3058-3068.(in Chinese)
[2] REVILLA I,VIVAR-QUINTANA A M,PALACIOS C,et al.Effects of rearing system (organic and conventional) and breed (Churra and Castellana) on fatty acid composition and sensory characteristics of suckling lamb meat produced in North-West Spain[J].Biological Agriculture & Horticulture,2020,36:1-15.
[3] DE BRITO G F,POMMANLALAM E N,HOPKINS D L.The effect of extensive feeding systems on growth rate,carcass traits,and meat quality of finishing lambs[J].Comprehensive Reviews in Food Science & Food Safety,2017,16:26-38.
[4] 张丽,孙宝忠.肌纤维类型与生理代谢、生长性能及肉质的关系[J].肉类研究,2013,27(8):25-30. ZHANG L,SUN B Z.Relationship of muscle fiber types with physiological metabolism,growth performance and meat quality[J].Meat Research,2013,27(8):25-30.(in Chinese)
[5] 贾安峰,冯京海,张敏红.调控骨骼肌肌纤维类型转化的因素及机制[J].动物营养学报,2014,26(5):1151-1156. JIA A F,FENG J H,ZHANG M H.Factors and mechanisms regulating the transformation of skeletal muscle fiber types[J].Chinese Journal of Animal Nutrition,2014,26(5):1151-1156.(in Chinese)
[6] 邵亚飞,贺琼玉,姚卫磊,等.山竹醇对生长育肥猪生长性能、肌肉抗氧化能力和肌纤维类型组成的影响[J].动物营养学报,2020,32(3):1151-1160. SHAO Y F,HE Q Y,YAO W L,et al.Effects of garcinol on growth performance,muscle antioxidant capacity and muscle fiber type of growing-finishing pigs[J].Chinese Journal of Animal Nutrition,2020,32(3):1151-1160.(in Chinese)
[7] 闫祥林,任晓镤,刘瑞,等.饲养方式对新疆多浪羊肉品质的影响[J].食品科学,2018,39(15):80-87. YAN X L,REN X P,LIU R,et al.Effects of different feeding modes on meat quality of Duolang sheep[J].Food Science,2018,39(15):80-87.(in Chinese)
[8] 张琪,徐炜琳,李娜,等.放牧与舍饲松辽黑猪胴体性能和肉质品质的比较[J].猪业科学,2016,33(9):128-129. ZHANG Q,XU W L,LI N,et al.Comparison of carcass performance and meat quality between grazing and house feeding Songliao Black pigs[J].Swine Industry Science,2016,33(9):128-129.(in Chinese)
[9] 尹丽卿.不同饲养方式下苏尼特羊肌纤维特性和MyHC基因对肉质的影响[D].呼和浩特:内蒙古农业大学,2016. YIN L Q.The effect of muscle fiber characteristics and MyHC gene on meat quality of Sunit sheep in different feeding conditions[D].Hohhot:Inner Mongolia Agricultural University,2016.(in Chinese)
[10] GENTRY J G,MCGLONE J J,MILLER M F,et al.Environmental effects on pig performance,meat quality,and muscle characteristics[J].Journal of Animal Science,2004,82(1):209-217.
[11] VESTERGAARD M,OKSBJERG N,HENCKEL P.Influence of feeding intensity,grazing and finishing feeding on muscle fibre characteristics and meat colour of semitendinosus,longissimus dorsi and supraspinatus muscles of young bulls[J].Meat Science,2000,54(2):177-185.
[12] 中华人民共和国农业部.中华人民共和国农业行业标准——肉羊饲养标准(NY/T 816-2004)[S].2004-09-01. MINISTRY OF AGRICULTURE OF THE PEOPLE'S REPUBLIC OF CHINA.The agricultural industry standard of the People's Republic of China:Feeding standard of meat-producting sheep and goats (NY/T 816-2004)[S].2004-09-01.(in Chinese)
[13] 周静静,马红彬,周瑶,等.轮牧方式对荒漠草原滩羊牧食特征、体重及繁殖性能的影响[J].中国农业科学,2017,50(8):1525-1534. ZHOU J J,MA H B,ZHOU Y,et al.Effects of different rotational grazing ways on grazing characteristics,weight and reproductive performance of Tan sheep in desert steppe[J].Scientia Agricultura Sinica,2017,50(8):1525-1534.(in Chinese)
[14] 仲俊涛,王蓓,米文宝,等.盐池县草地生态系统调查[J].干旱区资源与环境,2020,34(4):176-182. ZHONG J T,WANG B,MI W B,et al.Grassland ecosystem survey on Yanchi county[J].Journal of Arid Land Resources and Environment,2020,34(4):176-182.(in Chinese)
[15] 彭祺.荒漠草原草地放牧方式的试验研究[D].银川:宁夏大学,2005. PENG Q.Researeh on the diffcrent grazing systems in desert grassland[D].Yinchuan:Ningxia University,2005.(in Chinese)
[16] 中华人民共和国农业部.中华人民共和国农业行业标准——肉嫩度的测定剪切力测定法(NY/T 1180-2006)[S].2006-10-01. MINISTRY OF AGRICULTURE OF THE PEOPLE'S REPUBLIC OF CHINA.NY/T 1180-2006.Agricultural industry standard of the People's Republic of China——Determination of meat tenderness:Shear force measurement method[S].2006-10-01.(in Chinese)
[17] DE HUIDOBRO F R,MIGUEL E,BLAZQUEZ B,et al.A comparison between two methods (Warner-Bratzler and texture profile analysis) for testing either raw meat or cooked meat[J].Meat Science,2005,69(3):527-536.
[18] KIM G D,YANG H S,JEONG J Y.Intramuscular variations of proteome and muscle fiber type distribution in semimembranosus and semitendinosus muscles associated with pork quality[J].Food Chemistry,2018,244:143-152.
[19] 温凯欣,徐晨晨,张杨,等.宰后颈臂束缚技术对牛肉品质的影响[J].肉类研究,2020,34(1):34-38. WEN K X,XU C C,ZHANG Y,et al.Effect of postmortem neck-arm restraint on beef quality[J].Meat Research,2020,34(1):34-38.(in Chinese)
[20] CHOI Y M,YU Y C,KIM B C.Influence of myosin heavy and light chain isoforms on early postmortem glycolytic rate and pork quality[J].Meat Science,2007,76(2):281-288.
[21] LI K,ZHANG Y M,MAO Y W,et al.Effect of very fast chilling and aging time on ultra-structure and meat quality characteristics of Chinese Yellow cattle M.longissimus lumborum[J].Meat Science,2012,92(4):795-804.
[22] LAEMMLI U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J].Nature,1970,227(5259):680-685.
[23] PARK D,XIONG Y L,ALDERTON A L,et al.Biochemical changes in myofibrillar protein isolates exposed to three oxidizing systems[J].Journal of Agricultural and Food Chemistry,2006,54(12):4445-4451.
[24] 杨媛媛,李敬,赵青余,等.猪肌肉糖原酵解潜力的影响因素及其营养调控研究进展[J].中国畜牧杂志,2019,55(11):8-15. YANG Y Y,LIJ,ZHAO Q Y,et al.Factors affecting the glycolysis potential of pig muscle and its nutritional regulation[J].Chinese Journal of Animal Science,2019,55(11):8-15.(in Chinese)
[25] 徐少庭,徐晨晨,罗海玲.饲粮抗氧化剂对肌肉嫩度的影响及作用机制[J].动物营养学报,2017,29(8):2676-2680. XU S T,XU C C,LUO H L.Effects and mechanisms of dietary antioxidants on meat tenderness[J].Chinese Journal of Animal Nutrition,2017,29(8):2676-2680.(in Chinese)
[26] LUO Y,WANG B,LIU C,et al.Meat quality,fatty acids,volatile compounds,and antioxidant properties of lambs fed pasture versus mixed diet[J].Food Science & Nutrition,2019,7(9):2796-2805.
[27] LI J,YANG Y,ZHAN T,et al.Effect of slaughter weight on carcass characteristics,meat quality,and lipidomics profiling in longissimus thoracis of finishing pigs[J].LWT-Food Science and Technology,2021,140:1-10.
[28] FRENCH P,RIORDAN E G O,MONAHAN F J,et al.The eating quality of meat of steers fed grass and/or concentrates[J].Meat Science,2001,57(4):379-386.
[29] 王思飞,周玉香,张艳梅,等.日粮精粗比与共轭亚油酸对家畜生产性能的影响研究概况[J].畜牧与饲料科学,2017,38(11):35-37. WANG S F,ZHOU Y X,ZHANG Y M,et al.Research progress on effects of dietary forage to concentrate ratio and conjugated linoleic acid content on production performance of livestock[J].Animal Husbandry and Feed Science,2017,38(11):35-37.(in Chinese)
[30] SU R,LUO Y,WANG B,et al.Effects of physical exercise on meat quality characteristics of Sunit sheep[J].Small Ruminant Research,2019,183:1-6.
[31] 郝婉名,祝超智,赵改名,等.西门塔尔杂交牛不同部位肉间的差异性[J].肉类研究,2019,33(1):14-18. HAO W M,ZHU C Z,ZHAO G M,et al.Differences in meat quality among different parts of crossbred Simmental cattle[J].Meat Research,2019,33(1):14-18.(in Chinese)
[32] 郎玉苗,王勇峰,李敬,等.中国西门塔尔牛肌肉肌纤维类型和肉品质特性研究[J].中国畜牧兽医,2016,43(6):1489-1493. LANG Y M,WANG Y F,LI J,et al.Study on muscle fiber types and meat quality traits of Chinese Simmental cattle[J].China Animal Husbandry & Veterinary Medicine,2016,43(6):1489-1493.(in Chinese)
[33] 朱梦婷,王晓路,王永健,等.不同饲养方式对黄羽肉鸡肉品质的影响[J].江苏农业科学,2019,47(19):179-182. ZHU M T,WANG X L,WANG Y J,et al.Effects of different rearing methods on meat quality of Yellow-feather broiler chicken[J].Jiangsu Agricultural Sciences,2019,47(19):179-182.(in Chinese)
[34] 蔡洁琼,郑月,朱昊鹏,等.运动对肉鸡肉品质及肌纤维发育的影响[J].食品科学,2016,37(13):53-59. CAI J Q,ZHENG Y,ZHU H P,et al.Effect of exercise on broiler meat quality and muscle fiber development[J].Food Science,2016,37(13):53-59.(in Chinese)
[35] 王玉琴,田志龙,施会彬,等.湖羊肌肉营养特点及肌纤维组织学特性[J].动物营养学报,2017,29(8):2867-2874. WANG Y Q,TIAN Z L,SHI H B,et al.Nutritional characteristics and muscle fiber histological characters of muscle from Hu sheep[J].Chinese Journal of Animal Nutrition,2017,29(8):2867-2874.(in Chinese)
[36] 陈洁波,陶林,吴薇薇,等.贵妃鸡、怀乡鸡和北京油鸡肌纤维特性与肉品质相关性分析[J].中国畜牧兽医,2013,40(11):160-163. CHEN J B,TAO L,WU W W,et al.Analysis the relationship between muscle fiber and meat quality of Qrincess,Huaixiang and Beijing You chickens[J].China Animal Husbandry & Veterinary Medicine,2013,40(11):160-163.(in Chinese)
[37] 王亮,张宝善,李林强,等.猪牛羊肌肉组织质构特性差异比较及肌纤维分析[J].中国牛业科学,2016,42(3):34-38. WANG L,ZHANG B S,LI L Q,et al.Comparisons of texture properties of tissue and analysis of the muscle fibers on pork,beef and mutton[J].China Cattle Science,2016,42(3):34-38.(in Chinese)
[38] 苗建军,彭忠利,高彦华,等.青稞替代玉米对育肥牦牛生产性能和肉品质的影响[J].草业学报,2019,28(1):95-107. MIAO J J,PENG Z L,GAO Y H,et al.Effects on production performance,slaughter performance,apparent digestibility of nutrients and meat quality of partial substitution of corn concentrate with highland barley in a yak fattening system[J].Acta Prataculturae Sinica,2019,28(1):95-107.(in Chinese)
[39] 赵雅娟,尹丽卿,苏琳,等.不同饲养方式对苏尼特羊背最长肌肌纤维类型组成的影响[J].食品科学,2017,38(19):30-34. ZHAO Y J,YIN L Q,SU L,et al.Effect of different feeding systems on muscle fiber types of longissimus dorsi from Sunit sheep[J].Food Science,2017,38(19):30-34.(in Chinese)
[40] 蔡洁琼.运动训练对肉鸡生长性能、肉质性状及宰后肌肉腺苷酸代谢的影响[D].南京:南京农业大学,2016. CAI J Q.Effects of exercise training on growth performance,meat quality traits and muscle adenylate nucleotide metabolism in broilers[D].Nanjing:Nanjing Agricultural University,2016.(in Chinese)
[41] LI J,TANG C,ZHAO Q,et al.Integrated lipidomics and targeted metabolomics analyses reveal changes in flavor precursors in psoas major muscle of castrated lambs[J].Food Chemistry,2020,333:127451.
[42] ZELECHOWSKA E,PRZYBYLSKI W.Effect of feeding season on pH,glycolytic potential,colour and myofibrillar proteins in rabbit meat[J].Medycyna Weterynaryjna,2015,71(12):769-772.
[43] AALHUS J L,PRICE M A.Endurance-exercised growing sheep:Ⅰ.Post-mortem and histological changes in skeletal muscles[J].Meat Science,1991,29(1):43-56.
[44] GANGANT I D M,LEIBER F,DUFEY P A,et al.Physical activity,forced by steep pastures,affects muscle characteristics and meat quality of suckling beef calves[J].The Journal of Agricultural Science,2016,155(2):1-12.
[45] 尹靖东.动物肌肉生物学与肉品科学[M].北京:中国农业大学出版社,2011. YIN J D.Animal Muscle Biology and Meat Science[M].Beijing:China Agricultural University Press,2011.(in Chinese)
[46] XIONG G Y,FU X,PAN D,et al.Influence of ultrasound-assisted sodium bicarbonate marination on the curing efficiency of chicken breast meat[J].Ultrasonics Sonochemistry,2019,60:104808.
[47] MUROYA S,ERTBJERG P,POMPONIO L,et al.Desmin and troponin T are degraded faster in type Ⅱb muscle fibers than in type Ⅰ fibers during postmortem aging of porcine muscle[J].Meat Science,2010,86(3):764-769.
[48] FENG Y H,ZHANG S S,SUN B Z,et al.Changes in physical meat traits,protein solubility,and the microstructure of different beef muscles during postmortem aging[J].Foods,2020,9(6):806.
[49] 丰永红,王勇峰,李海鹏,等.新疆褐牛不同部位肉宰后成熟过程中蛋白降解变化研究[J].农业机械学报,2018,49(5):382-389. FENG Y H,WANG Y F,LI H P,et al.Protein degradation of different cuts in Xinjiang Brown cattle beef during postmortem aging[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(5):382-389.(in Chinese)
[50] 魏秀丽,谢小雷,张春晖,等.猪宰后肌肉体系中μ-calpain及肌原纤维蛋白理化特性的变化规律[J].中国农业科学,2015,48(12):2428-2438. WEI X L,XIE X L,ZHANG C H,et al.The variations in μ-calpain and physico-chemical characteristics of myofibrillar proteins in postmortem porcine muscle[J].Scientia Agricultura Sinica,2015,48(12):2428-2438.(in Chinese)
[51] HUFFLONERGAN E.Water-Holding capacity of fresh meat[J].Fact Sheet,2005,12:1-8.
[52] BOWKER B C,FAHRENHOLZ T M,SARNOSKI P J,et al.Alterations in the sarcoplasmic protein fraction of beef muscle with postmortem aging and hydrodynamic pressure processing[J].Journal of Food Science,2012,77(4-6):C594-C602.
[53] 门小明.肌肉纤维类型组成对猪肉品质的影响及其机理研究[D].无锡:江南大学,2012. MEN X M.Effects and underlying mechanisms of muscle fibre types on pork quality[D].Wuxi:Jiangnan University,2012.(in Chinese)

Effects of Concentrates and Pasture Feeding on Muscle Fiber Characteristics of Biceps Femoris in Tan Sheep and Its Protein Degradation During Postmortem Aging

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