绵羊心脏脂肪酸结合蛋白基因单核苷酸多态性分析

doi:10.16431/j.cnki.1671-7236.2018.01.015

Abstract

Abstract:

This experiment was aimed to study the genetic polymorphism of heart fatty acid binding protein (H-FABP) gene in sheep, and find the molecular marker-assisted selection. Tan sheep (250) and Tan×Hu hybrid F1 generation (174) were used as the experimental animals to detect single nucleotide polymorphism (SNP) of H-FABP gene (GenBank accession No.:AY157617) using SNaPshot technology. Gene frequency and genotype frequency statistics, Hardy-Weinberg equilibrium (HWE), expected heterozygosity (He), polymorphism information content (PIC) and effective number of alleles (Ne) were calculated. The correlation among the gene in different genotypes and body weight, body length, body height, chest circumference, chest depth, chest width and cannon circumference traits was analyzed. The results showed that 9 polymorphic loci were detected:939[A/G], 980[G/A], 1018[T/C], 2878[C/T], 2956[C/T], 3017[G/A], 3341[G/C], 3394[T/A] and 1056[-/G], including 6 transitions, 2 transversions and 1 single-base insertion. The He at 939[A/G], 980[G/A], 2956[C/T], 3341[G/C], 3394[T/A] and 1018[T/C] was 0.3200 to 0.4666, PIC ranged from 0.2688 to 0.3577; The He at 2878[C/T] and 3017[G/A] was 0.0283 to 0.1272, PIC ranged from 0.0279 to 0.1191 for low polymorphism; The He at 1056[-/G] in Tan sheep was 0.0120,whereas that of Tan×Hu hybrid F1 generation was 0, PIC in Tan sheep was 0.0119, whereas that of Tan×Hu hybrid F1 generation was 0.9 polymorphic loci in Tan sheep and Tan×Hu hybrid F1 generation followed Hardy-Weinberg equilibrium. 5 polymorphic loci of 939[A/G], 980[G/A], 2956[C/T], 3341[G/C] and 3394[T/A] were tightly linked (D'>0.99), and H-FABP gene was divided into 3 haplotypes:AA,AB and BB. BB haplotype had the maximum value in each production traits in 41 Tan×Hu hybrid F1 generation, but the difference was not significant (P>0.05). H-FABP gene in sheep had abundant genetic polymorphism,5 polymorphic loci of 939[A/G], 980[G/A], 2956[C/T], 3341[G/C] and 3394[T/A] tightly linked,BB haplotype might be the dominant haplotype associated with production traits in sheep.

Key words: Tan sheep; H-FABP gene; single nucleotide polymorphism (SNP); linkage disequilibrium (LD)

CLC Number:

S813.3

XIE Xiulan, LIANG Xiaojun, MA Qing, MA Xiaoming, MA Lina, YUE Caijuan, YANG Weidi, ER Hehua. Analysis of Single Nucleotide Polymorphism of Sheep H-FABP Gene[J]. , 2018, 45(1): 113-121.

References

[1] 钱成,罗卫星,黄兰,等. H-FABP基因在临床医学和畜牧生产上应用研究进展[J]. 基因组学与应用生物学,2015:34(7):1534-1540. QIAN C,LUO W X,HUANG L,et al. Application research progress of H-FABP gene in clinical medicine and livestock production[J]. Genomics and Applied Biology,2015,3(7):1534-1540. (in Chinese)
[2] 王同勋,施巧婷,徐照学. H-FABP基因多态性及其与肌内脂肪关联的研究进展[J]. 草食家畜,2009,2(6):1-3. WANG T X,SHI Q T,XU Z X. Research progress of the polymorphism of H-FABP gene and its association with the intramuscular fat[J]. Grass-Feeding Livestock, 2009, 2(6):1-3. (in Chinese)
[3] HUANG Z G,XIONG L,LIU Z S,et al. The developmental changes and effect on IMF content of H-FABP and PPAR γ mRNA expression in sheep muscle[J].Acta Genetica Sinica,2006,33(6):507-514.
[4] CHMURZYNSKA A. The multigene family of fatty acid binding proteins(FABPs):Function,structure and polymorphism[J]. Journal Applied Genetics,2002,47(1):39-48.
[5] 郝称莉,李齐发,乔永,等. 湖羊肌肉组织H-FABP和PPARγ基因表达水平与肌内脂肪含量的相关研究[J]. 中国农业科学,2008,41(11):3776-3783. HAO C L,LI Q F,QIAO Y,et al. Association of the H-FABP and PPARγ gene expression with intramuscular fat content in Hu sheep muscles[J]. Scientia Agricultura Sinica,2008,41(11):3776-3783.(in Chinese)
[6] 韦吉春,徐亚欧,毛亮,等. 藏绵羊脂肪酸结合蛋白(H-FABP)基因的克隆及序列分析[J]. 西南民族大学学报(自然科学版),2010,36(2):214-219. WEI J C,XU Y O,MAO L,et al. Tibetan sheep H-FABP gene clone and bioinformatics analysis[J].Journal of Southwest University for Nationalities (Natural Science Edition), 2010,36(2):214-219.(in Chinese)
[7] 徐红伟,柏家林,冯玉兰,等. 兰州大尾羊心脏型脂肪酸结合蛋白(H-FABP)基因克隆及其同源性比较[J].中国农业科学,2013,46(3):639-646. XU H W,BAI J L,FENG Y L,et al. Cloning and sequence analysis of the full-length cDNA of H-FABP gene in Lanzhou fat-tailed sheep[J]. Scientia Agricultura Sinica, 2013, 46(3):639-646.(in Chinese)
[8] 乔海云. 滩羊H-FABP基因和ADD1基因遗传多态性及其与肌内脂肪含量的关联分析[D]. 北京:中国农业科学院,2009. QIAO H Y. Genetic polymorphisms of H-FABP and ADD1 gene and the association with intramuscular fat content in tan sheep[D]. Beijing:Chinese Academy of Agricultural Sciences,2009.(in Chinese)
[9] 谢一妮. 山羊H-FABP基因多态性及其与肌内脂肪含量的相关性研究[D]. 雅安:四川农业大学,2011. XIE Y N. Studies on polymorphisms of H-FABP gene and the association with intramuscular fat content in the goats[D]. Ya'an:Sichuan Agriculture University,2011. (in Chinese)
[10] 钱成,罗卫星,孙岩岩,等. 贵州地方山羊品种H-FABP基因第3外显子SNP研究[J]. 生物技术,2015,25(4):349-354. QIAN C,LUO W X,SUN Y Y,et al. Study on SNP of H-FABP exon 3 of Guizhou local goat breeds[J]. Biotechnology, 2015, 25(4):349-354.(in Chinese)
[11] WANG S, CAI X, XUE K, et al. Polymorphisms of MRF4 and H-FABP genes association with growth traits in Qinchuan cattle and related hybrids[J]. Molecular Biology Reports,2011,38:1013-1020.
[12] 乔海云,赵倩君,姚娜,等. 绵羊H-FABP 基因单核苷酸多态性的研究[J]. 遗传,2009,31(7):725-731. QIAO H Y,ZHAO Q J,YAO N,et al. Studie on single nucleotid polymorphisms of H-FABP gene in sheep[J]. Hereditas,2009,31(7):725-731.(in Chinese)
[13] 赵雪,曹阳,金海国,等. 不同绵羊群体间心脏型脂肪酸结合蛋白基因第2外显子单核苷酸多态性的研究[J]. 中国畜牧兽医,2013,40(4):32-35. ZHAO X,CAO Y,JIN H G,et al. Studies on single nucleotide polymorphisms at the exon 2 of H-FABP gene among different sheep breeds[J]. China Animal Husbandry & Veterinary Medicine,2013,40(4):32-35.(in Chinese)
[14] 赵雪,曹阳,周国利,等. 绵羊H-FABP基因不同组织表达差异研究[J]. 黑龙江畜牧兽医,2014,3:60-63. ZHAO X,CAO Y,ZHOU G L,et al. Differential expression of the H-FABP gene in different tissues of sheep[J]. Heilongjiang Animal Science and Veterinary Medicine,2014,3:60-63. (in Chinese)
[15] 孙丽敏,赵佳,姜怀志. 不同群体肉羊背最长肌中H-FABP mRNA表达的比较研究[J]. 中国草食动物科学,2013,33(5):10-13. SUN L M,ZHAO J,JIANG H Z. Expression of H-FABP mRNA in the muscle of different sheep breeds[J]. China Herbivore Science,2013,33(5):10-13. (in Chinese)
[16] 赵金宇. 宁夏滩羊早期生长发育规律和生产性能研究[D]. 杨凌:西北农林科技大学,2013. ZHAO J Y. Study on the early growth-development and productivity of Tan sheep in Ningxia[D]. Yangling:Northwest Agriculture and Forestry University,2013.(in Chinese)
[17] CALVO J H, VAIMAN D,SAIDI-MEHTAR N, et al. Characterization, genetic variation and chromosomal assignment to sheep chromosome 2 of the ovine heart fatty acid-binding protein gene (FABP3)[J].Cytogenetic and Genome Research,2002,98(4):270-273.
[18] 曹阳,赵雪,于永生,等. 肉羊心型脂肪酸结合蛋白基因第2外显子多态性与肉质性状相关分析[J]. 东北农业大学学报,2013,44(9):30-34. CAO Y,ZHAO X,YU Y S,et al. Correlation analysis on polymorphisms and meat quality trait at the exon 2 of H-FABP gene in sheep[J].Journal of Northeast Agricultural University,2013,44(9):30-34.(in Chinese)
[19] 余刚,罗军,韩雪峰,等. 陕北白绒山羊H-FABP基因SNPs及其与生长、胴体性状的相关研究[J]. 畜牧兽医学报,2007,38(11):1154-1159. YU G,LUO J,HAN X F,et al. Study on SNPs of H-FABP gene and its relationship with growth and carcass traits in Shanbei White cashmere goats[J]. Acta Veterinaria et Zootechnica Sinica,2007,38(11):1154-1159. (in Chinese)
[20] 罗燕,谷新利,赵宗胜,等. 中国美利奴羊H-FABP基因多态性及其与部分肉质性状的相关性[J]. 动物学杂志,2013,48(6):964-970. LUO Y,GU X L,ZHAO Z S,et al. Single nucleotide polymorphism of H-FABP gene and its correlation with meat quality traits in Merino sheep[J].Chinese Journal of Zoology,2013,48(6):964-970. (in Chinese)
[21] 赵雪. 绵羊H-FABP基因单核苷酸多态性及其与肉用性状的关联分析[D]. 延吉:延边大学,2013. ZHAO X. Single nucleotide polymorphism of H-FABP gene in sheep and its association with meat traits[D]. Yanji:Yanbian University,2013.(in Chinese)
[22] 王兰萍,耿荣庆,冀德君,等. 江苏地方山羊品种H-FABP基因的SNPs检测及群体遗传学分析[J]. 中国畜牧兽医,2012,39(3)159-162. WANG L P,GENG R Q,JI D J,et al.SNPs detection and genetic analysis on H-FABP gene of Jiangsu local goat breeds[J]. China Animal Husbandry & Veterinary Medicine,,2012,39(3)159-162.(in Chinese)
[23] 王兰萍,耿荣庆,冀德君,等. H-FABP基因多态性与江苏地方山羊品种IMF含量的关系[J]. 生物技术,2012,22(2):46-49. WANG L P,GENG R Q,JI D J,et al. Polymorphism of H-FABP gene and its relationship with IMF content in Jiangsu indigenous goat breeds[J]. Biotechnology,2012,22(2):46-49.(in Chinese)
[24] 王启贵,关天竹,王守志,等. A-FABP基因多态性与肉鸡生长和体组成性状的关联[J]. 遗传,2011,33(2):153-162. WANG Q G,GUAN T Z,WANG S Z,et al. Association between chicken A-FABP gene polymorphisms and growth and body composition traits[J]. Hereditas,2011,33(2):153-162.(in Chinese)

Analysis of Single Nucleotide Polymorphism of Sheep H-FABP Gene

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