›› 2017, Vol. 44 ›› Issue (9): 2558-2565.doi: 10.16431/j.cnki.1671-7236.2017.09.003

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PCR Amplification and Bioinformatics Analysis of IL-7 Gene in Tibetan Sheep

ZHANG Xiao-fen1,2, YE Gui-sheng1,2   

  1. 1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China;
    2. Department of Veterinary Medicine, College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
  • Received:2017-06-02 Online:2017-09-20 Published:2017-09-22

Abstract:

In order to get the interleukin-7 (IL-7) gene sequence of Tibetan sheep, and research the characteristics of this sequence and structure and function of encoding protein, IL-7 gene was amplified from Tibetan sheep by RT-PCR. The nucleotide sequence,amino acid sequence, homology and phylogenetic tree were analyzed by the DNAStar software. The secondary and tertiary structures, hydrophilicity, signal peptide and post-translational modification site of the encoding protein were predicted by DNAStar software and online servers. The results showed that the length of IL-7 gene was 531 bp (contained termination codon), and encoded 176 amino acids. Compared with IL-7 gene of Ovis aries, Capra hircus, Pantholops hodgsonii, Bubalus bubalis, Bos indicus, Bison bison bison, Bos taurus and Bos mutus, IL-7 gene of Tibetan sheep showed a great similarity from 97.2% to 99.8%, the amino acid sequence homology varied from 94.9% to 99.4%, and the relationship was the closest between Tibetan sheep and Ovis aries. Result from protein structure prediction indicated that the IL-7 protein was mainly composed of α-helix, it was a hydrophilic and secretory protein. Furthermore, it had six kinds of post translational modification sites, including one N-myristoylation site, one amidation site, one cAMP-and cGMP-dependent protein kinase phosphorylation site, three N-glycosylation sites, four protein kinase C phosphorylation sites and six casein kinase Ⅱ phosphorylation sites. These results might provide references for further study and clinical application of IL-7 gene in Tibetan sheep.

Key words: Tibetan sheep; IL-7 gene; RT-PCR; sequence analysis; protein structure prediction

CLC Number: