云南边境地区帕利亚姆血清群中山病病毒的分离与鉴定

doi:10.16431/j.cnki.1671-7236.2022.11.031

Abstract

Abstract: 【Objective】 This study was aimed to understand the situation of cattle infected with Chuzan virus (CHUV) in the southwest frontier area.【Method】 BHK21 cells were used to isolate virus from the blood samples of healthy cattle collected in Jinghong city and Jiangcheng county, Yunnan province.The samples with cytopathy were identified by morphology, genomic banding and molecular biology.The sequences of S7 and S2 genes were determined and compared.【Result】 Four samples with cytopathy were found.The virus particles were spherical with a diameter of about 50 nm by electron microscopy.The results of agarosegel electrophoresis showed that the genome of the four newly isolated viruses was 10 segments and the band was "3-3-4", which was similar to the strain of CHUV SZ187 isolated from Shizong in Yunnan province in 2012.The nucleotide similarity of the S7 and S2 genes of the four viruses were 98.7%-99.7%, 97.9%-99.1%.The sequence of S2 fragment had the highest homology with SZ187 and GHN-GS-16 strains isolated in China.The nucleotide and amino acid sequence of S7 gene had the highest homology with ON-1/E/18, ON-3/E/17 in Japan and SZ187, GHN-GS-16 in China.The results of genetic evolution analysis of S7 and S2 genes showed that the four newly isolated strains were the closest relatives.The S7 gene sequences of the four strains were in the same branch as the known PALV strains isolated from China and Japan, indicating that the four strains were PALV and formed a cluster, which had a certain regional character.The four strains were further confirmed to be CHUV, because the sequence of S2 gene of the four strains was on the same branch as the isolated CHUV from China.【Conclusion】 This study first reported the isolation of CHUV from cattle in the border area of Yunnan province.It provided an important reference and a powerful basis for the epidemiology of CHUV in China and the prevention and control of epidemic disease risk in border areas.

Key words: Chuzan virus(CHUV); isolation and identification; sequence analysis

CLC Number:

S852.65^＋9.4

KOU Meiling, MIAO Haisheng, LI Le, XIE Jiarui, LI Huachun, SONG Jianling. Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4401-4409.

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Isolation and Identification of Chuzan Virus from Palyam Serogroup Virus in Yunnan Border Area

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