禽腺病毒血清4型贵州株全基因序列分析

doi:10.16431/j.cnki.1671-7236.2021.01.005

Abstract

Abstract: To comprehend the molecular evolution of fowl adenovirus serotype 4 (FAdV-4) endemic strains,based on FAdV-4 GZ-BJ and FAdV-4 GZ-QL strains isolated in the laboratory,segmented amplification was performed on the two FAdV-4 strains,the amplified product was cloned into the vector,the plasmid was extracted,PCR and double enzyme digestion were performed,and the recombinant plasmid was screened for sequencing.The sequencing results were sequentially spliced to obtain the whole genome of FAdV-4 Guizhou strain,and sequence and genetic evolution analysis.The results showed that the lengths of two FAdV-4 Guizhou strains,GZ-BJ and GZ-QL strains,were 43 352 and 43 723 bp,were obtained by PCR fragmented amplification.respectively,and the complete genes of FAdV-4 GZ-BJ strain was 371 bp shorter than the FAdV-4 GZ-QL strain,and 6 ORFs (22K,putative 9.1 ku,u-exon,ORF17,ORF28,ORF42),and the amino acid homology of them was 57.1%.The nucleotide homology of the two FAdV-4 Guizhou strains with FAdV-4 strains in different regions at home and abroad were 88.7%-100%.The FAdV-4 two Guizhou strains and domestic isolates lacked ORF19,ORF27,ORF30 that compared with the FAdV-4 classic strain ON1.Phylogenetic tree analysis showed that the two Guizhou strains FAdV-4 GZ-BJ and FAdV-4 GZ-QL still belonged to group Ⅰ species C fowl adenovirus.This research indicated that the two Guizhou strains FAdV-4 GZ-BJ strain and FAdV-4 GZ-QL strain had evolution and mutations compared with domestic and foreign FAdV-4 strains,and FAdV-4 GZ-BJ strain had changed greatly,but not yet changing its serotype and provided a basis for exploring the molecular mechanism of FAdV-4 virus pathogenic mechanism.

Key words: fowl adenovirus serotype 4 (FAdV-4); sequence analysis; variance analysis

CLC Number:

S852.65

YIN Dejing, ZHANG Yundan, YUE Jun, LI Qiaobin, HE Ling, LI Tao, WANG Kaigong, CHENG Zhentao. Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 44-54.

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Whole Genomic Sequencing Analysis of Fowl Adenovirus Serotype 4 from Guizhou

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