猪德尔塔冠状病毒CH7328株的致病性及全基因组序列分析

doi:10.16431/j.cnki.1671-7236.2021.11.024

Abstract

Abstract: In order to understand the pathogenicity of Porcine deltacoronavirus (PDCoV) CH7328 strain, and analyze its genetic variation and genetic evolution, this test used virus culture, indirect immunofluorescence assay (IFA), virus growth curve and piglet pathogenicity test to identify the growth characteristics and pathogenicity of the virus, and analyze the entire genome sequence of the virus strain. The results showed that ST cells infected with PDCoV CH7328 strain showed swelling and rounding, the cells were broken glass-like and brushed, and the cells clustered and fell off. The results of the IFA test showed that the PDCoV CH7328 strain was mainly distributed in the cytoplasm. The growth curve results showed that the PDCoV CH7328 strain began to proliferate at 6 h, and reached a peak at 36 h, the corresponding TCID₅₀ was 10^-8.0/mL, and then gradually decreased. The pathogenicity results of piglets showed that the piglets in challenge group showed clinical symptoms such as diarrhea, dehydration and anorexia, the small intestine was obviously thinner, transparent and full, and the small intestinal villi were severely shed, atrophy or reduced, and intestinal villi cells were necrotic. The results of fecal pathogen detection showed that the virus could be detected on the second day of the challenge, after which the detoxification continued to increase, while the normal control piglets did not have any clinical signs. Whole genome sequencing and sequence analysis showed that the full length of the genome of the PDCoV CH7328 strain was 25 420 bp, which was the highest similar to the domestic GD strain, reaching 99.9%. The genetic evolution results showed that the PDCoV CH7328 strain was in the same evolutionary branch with domestic HKU15-155, CHN-HN-2014 and GD strains, and the genetic relationship was the closest. The results of this study provided a reference data and theoretical basis for the screening of PDCoV vaccine candidate strains and the prevention and control of pig farm diseases in my country.

Key words: Porcine deltacoronavirus (PDCoV); CH7328 strain; pathogenicity; whole gene sequence analysis

CLC Number:

S852.65⁺1

WANG Zhengfan, ZHU Lisai, LI Fei, XIANG Rui, LI Yiyun, YING Biyun, WANG Guiping, BAI Aiquan, JIA Aiqing. Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4143-4153.

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Pathogenicity and Whole Genome Sequence Analysis of Porcine Deltacoronavirus CH7328 Strain

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