豪猪源肠侵袭型大肠杆菌的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2022.11.028

Abstract

Abstract: 【Objective】 This study was aimed to identify the pathogenic bacteria and its characteristics that caused diarrhea and death of porcupine in a porcupine farm in Longyan city, Fujian province, and provide a reference for scientific prevention and control of the disease and rational drug use.【Method】 The pathogens of diarrhea-dead porcupines were isolated, and the isolates were identified by combining morphological characteristics, physiological and biochemical tests, 16S rRNA gene amplification and phylogenetic grouping.The pathogenicity and drug resistance of the isolates were studied by virulence gene detection, animal pathogenicity test and drug sensitivity test.【Result】 A strain of Escherichia coli was isolated from liver of the morbid and dead porcupine, and named as Fj/Porcupine2018.The sequence homology between the isolate and 22 reference strains from different sources of 16S rRNA was 97.4% to 99.8%.Among them, the sequence homology with the avian isolate (accession No.:MN022583) and the human isolate (accession No.:MW881377) were as high as 99.8%.The phylogenetic grouping confirmed that the isolate belonged to B1 group.The virulence gene test results showed that that in addition to the enteroinvasive Escherichia coli virulence gene EinV, 6 virulence-related genes of papC, iroC, Afa, luxs, stx2f and ompA were also detected.The results of animal pathogenicity tests showed that the isolate had strong virulence to the challenged mice, and all the mice died within 31 h after the challenge, and obvious pathological damage was caused to liver, spleen, lung, intestine and other organs and tissues of the dead mice.In the drug susceptibility test, the isolate showed different degrees of resistance to 7 classes of 11 drugs, including macrolides, tetracyclines, quinolones, sulfonamides, cephalosporins, aminoglycosides and penicillins, the drug resistance rate was 25% to 100%, and it was only sensitive to ceftriaxone and ceftriaxone.【Conclusion】 In this study, a pathogenic Escherichia coli strain of diarrhea-dead porcupines was isolated and obtained.Through a series of biological characteristics studies, it was confirmed that the isolate was a group B1 enteroinvasive Escherichia coli with strong pathogenicity and multi-drug resistance, which provided a certain reference for the prevention and control of colibacillosis in wild animals such as porcupine.

Key words: porcupine; enteroinvasive Escherichia coli; isolation and identification; pathogenicity; drug sensitivity test

CLC Number:

S852.61⁺2

LI Yuqi, XU Liting, QUE Qunyan, LIAO Xin, ZHENG Xin, YUAN Qingyang, WU Linyue, XIE Xinjie, TIAN Ying, YANG Shoushen, LI Xiaohua, DAI Ailing, YANG Xiaoyan, FAN Kewei. Isolation,Identification and Pathogenicity of Enteroinvasive Escherichia coli from Porcupine[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4372-4382.

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Isolation,Identification and Pathogenicity of Enteroinvasive Escherichia coli from Porcupine

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