果子狸源细小病毒分离鉴定及VP2基因遗传进化分析

doi:10.16431/j.cnki.1671-7236.2022.10.034

Abstract

Abstract: 【Objective】 The purpose of the experiment was to explore the epidemic characteristics of Parvovirus from palm civets and the genetic variation of VP2 gene,so as to provide theoretical basis and technical support for Parvovirus control.【Method】 CRFK cells were used to isolate the virus from the intestines of palm civets with enteritis.The virus was identified by hemagglutination test and immunofluorescence test.The VP2 gene of the virus was amplified by PCR and its genetic variation was analyzed.【Result】 Typical cytopathic effects (CPE)of Parvovirus such as cell abscission,disintegration and fragmentation were observed in CRFK cells.The results of hemagglutination test showed that the virus could agglutinate porcine erythrocytes,and the hemagglutination titer was 1∶512.The results of immunofluorescence test showed that bright green fluorescence could be observed in the CRFK cells inoculated with the isolated strain,while no fluorescence was observed in the control cells.The isolated strain was named MPCPV-SX.Phylogenetic tree analysis of VP2 gene showed that it was in the same branch as Canine parvovirus,located between CPV-2 and CPV-2a.At the same time,the 87 and 101 amino acids of VP2 were CPV-2,while the 300 and 305 amino acids were consistent with CPV-2a.【Conclusion】 In this study,a strain of Parvovirus MPCPV-SX was successfully isolated from the intestines of palm civets.Its VP2 gene was an intermediate type between CPV-2 and CPV-2a,indicating that Parvovirus spreaded across the host through palm civets and other wild animals,which might play a transitional role in the genetic evolution of parvovirus.

Key words: Parvovirus; palm civets; isolation and identification; VP2 gene; sequence analysis

CLC Number:

S852.65^＋9.2

TAN Bin, WANG Chao, WANG Yan, ZHANG Shuqin. Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4012-4018.

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Isolation and Identification of Parvovirus from Palm Civets and Sequence Analysis of VP2 Gene

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