1株大黄鱼源金黄杆菌的分离鉴定和耐药性分析

doi:10.16431/j.cnki.1671-7236.2022.03.036

Abstract

Abstract: 【Objective】 The study was aimed to explore the pathogens, pathogenicity and drug resistance of Pseudosciaena crocea in a farm in Zhejiang province.【Method】 The diseased Pseudosciaena crocea was dissected under sterile conditions, the liver and kidney tissues were taken, and the bacteria were isolated and cultured by scribing.The species characteristics of the isolated bacteria were identified by morphological observation, physiological and biochemical test and 16S rRNA gene sequence analysis.The pathogenicity of the isolated bacteria to Pseudosciaena crocea was evaluated by artificial infection and tissue section.Finally, the drug resistance of the isolated bacteria was detected by drug sensitive paper method.【Result】 The appearance of the isolated bacteria on the culture medium was yellow round colonies with convex, smooth surface and neat edges.After Gram staining, there were single scattered or paired distribution of Corynebacterium red under an optical microscope.The isolated strain could grow on MacConkey agar and produce acid by glucose.16S rRNA gene sequence similarity analysis and phylogenetic tree showed that the isolated strain had more than 97.0% similarity with Chryseobacterium sp. and clustered into one class.The results of animal regression test showed that the isolated bacteria could cause obvious lesions in the liver, kidney and spleen of Pseudosciaena crocea, and the median lethal dose was 6.32×10⁴ CFU/mL, indicating strong pathogenicity to Pseudosciaena crocea.The results of drug sensitivity showed that the isolated bacteria were sensitive to amikacin and levofloxacin, moderately sensitive to gentamicin, neomycin and erythromycin, and resistant to cefixime, ampicillin, kanamycin and furazolidone.【Conclusion】 In this study, the pathogenic strain of Chryseobacterium isolated from diseased Pseudosciaena crocea was reported, and its pathogenicity and drug resistance were studied to provide references for the prevention, control and treatment of aquatic animal diseases caused by Chryseobacterium sp..

Key words: Chryseobacterium sp.; isolation and identification; artificial infection; sequence analysis; drug resistance

CLC Number:

S941.42

CAI Jie, WU Jiayi, PAN Yuanxia, HU Gaowei, ZHANG Linzhou. Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1135-1143.

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Isolation, Identification and Drug Resistance Analysis of Chryseobacterium sp. from Pseudosciaena crocea

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