日粮能量及蛋白质水平对滩羊糖类转运载体mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2017.06.012

Abstract

Abstract:

This experiment was conducted to study the effects of the diets with different energy and protein levels on mRNA expression of glucose transporters in the small intestine and muscle of Tan sheep. A total of 112 healthy Tan sheep (half male and half female) with similar initial live weight were randomly divided into four groups with four replicates per group and seven sheep per replicate. According to the Feeding Standard of Meat-producing Sheep and Goats (NY/T 816—2004),each group was fed diet with different levels of energy and protein respectively:0.84×standard level (group Ⅰ),0.96×standard level (group Ⅱ),1.08×standard level (group Ⅲ) and 1.20×standard level (group Ⅳ). The test period were divided into two stages by body weight of sheep (29-35 and 36-40 kg). At the end of each stage,one sheep was slaughtered at each replicate,and small intestine and muscle samples were collected to study mRNA relative expression of SGLT1,GLUT4 and GLUT5 genes by Real-time PCR. The results indicated that:SGLT1 gene mRNA expression levels of group Ⅲ was significantly higher than other groups in small intestine at the end of 29-35 kg stage (P< 0.05);At the end of 36-40 kg stage,the SGLT1 gene mRNA expression levels had no significant difference among the four groups (P> 0.05).In muscle,the SGLT1 gene mRNA expression level increased with the rise of energy and protein levels at both stages ,and that of group Ⅳ were the highest.In small intestine,at the end of 29-35 kg stage,the GLUT4 gene mRNA expression levels had no significant difference among the four groups (P> 0.05);At the end of 36-40 kg stage,the GLUT4 gene mRNA expression level increased with the rise of energy and protein levels,and that of group Ⅳ were significantly higher than the other groups (P< 0.05).In muscle,the GLUT4 gene mRNA expression level were the highest in group Ⅳ at both stages,and significantly higher than group Ⅰ (P< 0.05). The GLUT5 gene mRNA expression did not show any regularity at both stages. In conclusion,diets with different levels of energy and protein could significantly affect the mRNA expression of SGLT1 and GLUT4 genes,and affect absorption of glucose in sheep.

Key words: Tan sheep; energy; protein; glucose transporters

CLC Number:

S816.4

LU Yan-juan, WANG Xiao-fang, ZENG Jie, CHEN Yu-lin, ZHANG En-ping. Effects of Diets with Different Energy and Protein Levels on mRNA Expression of Glucose Transporters in Tan Sheep[J]. , 2017, 44(6): 1657-1665.

References

[1] Kahn B B.Facilitative glucose transporters:Regulatory mechanisms and dysregulation in diabete[J]. J Clin Invest,1992,89(5):1367-1374.
[2] 冯定远.猪肌肉碱性氨基酸和葡萄糖转运载体cDNA克隆及mRNA表达调控的研究[D].南京:南京农业大学,2009.
[3] 周瑞宇,辛现良,耿美玉.葡萄糖转运载体及其在单糖肠吸收中的作用研究进展[J].现代生物医学进展,2010,10(17):3335-3339.
[4] 张爱华.肉仔鸡肠道主要营养素转运载体mRNA表达发育规律的研究[D].北京:中国农业科学院,2014.
[5] Wood I S,Trayhurn P.Glucose transporters(GLUT and SGLT):Expanded families of sugar transport proteins[J].Journal of Nutrition,2003,89(1):3-9.
[6] 石常友,王文策,耿梅梅,等.不同蛋白质水平日粮对肥育猪肠道氨基酸转运载体CAT1 mRNA表达量的影响[J].动物营养学报,2008,20(6):692-698.
[7] Humphrey B D,Stephensen C B,Calvert C C,et al. Glucose and cationnic amino acid transporter expression in growing chickens (Gallus gallus domesticus)[J].Comparative Biochemistry and Physiology Part A: Molecular &Integrative Physiology,2004,138(4):515-525.
[8] Ferraris R P. Dietary and developmental regulation of intestinal sugar transport[J].Biochem J,2001,360(2):265-276.
[9] Drozdowski L A,Thomson A B. Intestinal sugar transport[J]. World J Gastroenterol,2006,12(11):1657-1670.
[10] Kozakai T,Imura K,Nakajima K,et al.The effects of fasting and grazing on Na-Glucose cotransporter-1(SGLT-1) gene expression of rectal epithelia in beef cattle[J]. Asian-Aust J Anim Sci,2009,22(2):232-237.
[11] Vayro S,Wood I S,Dyer J,et al.Transcriptional regulation of the ovine intestinal Na⁺/glucose cotransporter SGLT1 gene[J].Eur J Biochem,2001,268(20):5460-5470.
[12] Dyer J,Vayro S,King T P,et al. Glucose sensing in the intestinal epithelium[J].Eur J Biochem,2003,270(16):3377-3388.
[13] Roder P V,Geillinger K E,Zietek T S,et al. The role of sglt1 and glut2 in intestinal glucose transport and sensing[J].PLoS One,2014,9(2):e89977.
[14] Lescalematys L,Dyer J,Scott D,et al. Regulation of the bovine intestinal Na⁺/glucose co-transporter (SGLT1) is dissociated from mRNA abundance[J].Biochem J,1993,291(2):435-440.
[15] Shirazi-Beechey S P,Gribble S M,Wood I S,et al.Dietary regulation of the intestinal sodium-dependent glucose cotransporter(SGLT1)[J].Biochem Soc Trans,1994,22(3):655-658.
[16] Rodriguez S M,Guimaraes K C,Matthews J C,et al.Influence of abomasal carbohydrates on small intestinal sodium-dependent glucose cotransporter activity and abundance in steers[J].J Anim Sci,2004,82(10):3015-3023.
[17] Zhao F Q,Okine E R,Cheeseman C I,et al.Glucose transporter gene expression in lactating bovine gastrointestinal tract[J].J Anim Sci,1998,76(11):2921-2929.
[18] Zhao F Q,Zheng Y C,Wall E H,et al.Cloning and expression of bovine sodium/glucose cotransporters[J].J Dairy Sci,2005,88(1):182-194.
[19] Ferraris R P,Diamond J M.Crypt/villus site of substrate-dependent regulation of mouse intestinal glucose transporters[J].PNAS,1993,90(12):5868-5872.
[20] Reimer R A,Field C J,McBurmey M I,et al.Ontogenic changes in proglucagon mRNA in BB diabetes prone and normal rats weaned onto a chow diet[J].Diabeologia,1997,40(8):871-878.
[21] 张航,刘强,王聪,等.2-甲基丁酸对犊牛小肠酶活性及葡萄糖转运载体基因表达的影响[J].中国农业科学,2016,49(5):979-987.
[22] 张霞,孙海洲,桑丹,等.饲粮营养水平对绒山羊小肠感应因子mRNA 相应表达量、血液理化指标及激素含量的影响[J].动物营养学报,2016,28(8):2607-2618.
[23] 田春丽.不同能量和蛋白水平日粮对滩羊生长性能、养分消化率和经济效益的影响[D].杨凌:西北农林科技大学,2015.
[24] 李文清,王加启,南雪梅,等.奶牛乳糖合成及泌乳相关基因和细胞信号通路的研究进展[J].中国畜牧兽医,2012,39(11):104-111.
[25] Menoyo D,Diez A,Lopez-Bote C J,et al.Dietary fat type affects lipid metabolism in atlantic salmon(salmo salar1.) and differentially regulates glucose tranporter glut4 expression in muscle[J].Aquaculture,2006,261(1):294-304.
[26] Gardan D,Gondret F,Louveau I.Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes[J].Ajp Endocrinology & Metabolism,2006,291(2):222-229.
[27] 周世卫.日粮能蛋水平对4~8月龄滩羊育肥效果及优质切块比例的影响[D].杨凌:西北农林科技大学,2016.
[28] 代立霞.不同日粮对滩羊尾部脂肪沉积及脂肪代谢相关基因表达的影响[D].杨凌:西北农林科技大学,2016.
[29] Wildersmith C H,Li X,Ho S,et al. Fructose transporters GLUT5 and GLUT2 expression in adult patients with fructose intolerance[J].United European Gastroenterology Journal,2014,2(1):14-21.
[30] Kishi K,Tanaka T,Igawa M,et al.Sucrase-isomaltase and hexose transporter gene expression are coordinately enhanced by dietary fructose in rat jejunum[J].J Nutr,1999,129(5):953-956.
[31] Douard V,Ferraris R P. The role of fructose transporters in diseases linked to excessive fructose intake[J].Journal of Physiology,2013,591(2):401-414.

Effects of Diets with Different Energy and Protein Levels on mRNA Expression of Glucose Transporters in Tan Sheep

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