一周岁内滩羊皮肤差异表达蛋白研究

doi:10.16431/j.cnki.1671-7236.2017.04.007

Abstract

Abstract:

In order to study different expressed proteins of one-year-old Tan sheep skin, two-dimensional gel electrophoresis (2-DE) was used to separate the Tan sheep skin proteins of different growth stages by Coomassie blue staining, with PDQuest 8.0 software to detect and analyze differences in protein spots,and were identified by MALDITOF/TOF-MS/MS.Protein maps of four growth stages were performed between pairwise, after testing found a total of 19 differentially expressed proteins, mass spectrometry successfully identified 15 protein spots and a total of 13 proteins, of which 14-3-3 σ protein isoforms, annexin A2, keratin 25 and tubulin α chain might be related to growth regulation sheep skin. Those different expressed proteins might be associated with different stages of growth of Tan sheep skin follicles cyclical changes. The different proteins of one-year-old Tan sheep skin provided theoretical basis for annual variation of Tan sheep skin.

Key words: Tan sheep; skin; two-dimensional electrophoresis; differential proteins

CLC Number:

S826

YANG Zuo-qing, TAO Jin-zhong, CHEN Xin, MA Rui-hua, ZHANG Xin-rong. Study on Skin Differential Expression Proteins of Tan Sheep Within One Year Old[J]. , 2017, 44(4): 986-993.

References

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Study on Skin Differential Expression Proteins of Tan Sheep Within One Year Old

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