基于双向电泳技术研究湖羊初乳和常乳乳蛋白质组差异

doi:10.16431/j.cnki.1671-7236.2021.03.010

Abstract

Abstract: The objective of this experiment was to analyze the differential proteomic between colostrum and mature milk of sheep,and provide further insight into the proteome characteristics during different lactation periods.6 Hu sheep with the same parity and similar due date were selected to collect colostrum on the 1st,3rd,7th day and mature milk on the 14th,28th,56th day postpartum.6 units of milk were mixed as a tested sample with equal volume at each time point,and defatted milk was prepared by removing the fat from the whole milk.The defatted milk of colostrum and mature milk were analyzed by two-dimensional electrophoresis (2-DE).The results showed that the content of milk protein was higher in colostrum,and decreased with the extension of lactation stage.According to the 2-DE map of milk protein on the 1st day,38 different protein spots were detected on the 3rd day,20 of which only existed on the 1st day and 1 only existed on the 3rd day,the other 17 protein spots showed differential expression.On the 7th day,35 different protein spots were detected,19 of which only existed on the 1st day and 1 only existed on the 7th day,the other 15 protein spots showed differential expression.On the 14th day,34 different protein spots were detected,11 of which only existed on the 1st day and 1 only existed on the 14th day,the other 22 protein spots showed differential expression.On the 28th day,38 different protein spots were detected,28 of which only existed on the 1st day and 1 only existed on the 28th day,the other 9 protein spots showed differential expression.On the 56th day,36 different protein spots were detected,26 of which only existed on the 1st day and 1 only existed on the 56th day,the other 9 protein spots showed differential expression.To sum up,a total of 44 differentially expressed protein spots were detected,8 of which only existed on the 1st day of postpartum,and these proteins were mainly involved in immunological activity.

Key words: two-dimensional electrophoresis (2-DE); Hu sheep; colostrum; mature milk; proteomic

CLC Number:

S826

WENG Xiuxiu, LIU Xinxin, YUE Xiangpeng, LI Wanhong, LI Fadi. Differential Proteomic Study of Colostrum and Mature Milk of Hu Sheep Based on Two-dimensional Electrophoresis Technology[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 865-872.

References

[1] 高佳媛,邵玉宇,王毕妮,等.羊奶及其制品的研究进展[J].中国乳品工业,2017,45(1):34-38. GAO J Y,SHAO Y Y,WANG B N,et al.Research development of goat milk and its products[J].China Dairy Industry,2017,45(1):34-38.(in Chinese)
[2] 李贺,马莺.羊乳营养及其功能性特征[J].中国乳品工业,2017,45(1):29-33. LI H,MA Y.Nutrition and functional properties of goat milk[J].China Dairy Industry,2017,45(1):29-33.(in Chinese)
[3] 丁蕊艳,郭长英,范丽霞,等.牛乳及三种特色生鲜乳的蛋白质营养价值评价[J].山东农业科学,2018,50(2):124-127. DING R Y,GUO C Y,FAN L X,et al.Evaluation on protein nutritional value in cow milk and other three kinds of special raw milk[J].Shandong Agricultural Sciences,2018,50(2):124-127.(in Chinese)
[4] 李晓林,牛志刚,袁燕,等.湖羊、小尾寒羊泌乳关键期泌乳性状的测定与比较[J].江西农业学报,2016,28(1):114-118. LI X L,NIU Z G,YUAN Y,et al.Determination and comparison of milk secretion traits of Hu sheep and Han sheep in lactation key period[J].Acta Agriculturae Jiangxi,2016,28(1):114-118.(in Chinese)
[5] GEORGIEV I P.Differences in chemical composition between cow colostrum and milk[J].Bulgarian Journal of Veterinary Medicine,2008,11(1):3-12.
[6] 乌兰君,杨梅,王满霞,等.牛初乳与牛乳中乳清蛋白质组成及功能的对比分析[J].现代食品科技,2017,33(5):58-63. WU L J,YANG M,WANG M X,et al.Comparative analysis of composition and function of whey proteins in bovine colostrum and bovine milk[J].Modern Food Science and Technology,2017,33(5):58-63.(in Chinese)
[7] 刘欣欣,李发弟,乐祥鹏.羊奶成分和奶中主要蛋白的研究进展[J].中国畜牧杂志,2016,52(9):87-91. LIU X X,LI F D,YUE X P.Research progress on of milk composition and analysis of main proteins in goat and sheep milk[J].Chinese Journal of Animal Science,2016,52(9):87-91.(in Chinese)
[8] MORENO-INDIA I,SANCHEZ-MACIAS D,CASTRO N,et al.Chemical composition and immune status of dairy goat colostrum fractions during the first 10 h after partum[J].Small Ruminant Research,2012,103:220-224.
[9] SANCHEZ-MACIAS D,MORENO-INDIAS I,CASTRO N,et al.From goat colostrum to milk:Physical,chemical,and immune evolution from partum to 90 days postpartum[J].Journal of Dairy Science,2014,97(1):10-16.
[10] 刘欣欣.湖羊不同泌乳期乳蛋白质组差异的研究[D].兰州:兰州大学,2016. LIU X X.The differential proteomic study of Hu sheep milk in different lactation period[D].Lanzhou:Lanzhou University,2016.(in Chinese)
[11] 陈静廷,卜登攀,马露,等.不同等电点沉淀法和超速离心法提取牛奶乳清蛋白的双向电泳分析[J].食品科学,2014,35(20):180-184. CHEN J T,BU D P,MA L,et al.2-DE analysis of whey protein extracted by different isoelectric precipitations and ultracentrifugation methods from cow milk[J].Food Science,2014,35(20):180-184.(in Chinese)
[12] 刘鸣畅,吴亚君,王斌,等.采用双向电泳技术研究牛乳热加工后蛋白质组变化[J].中国乳品工业,2016,44(3):23-26. LIU M C,WU Y J,WANG B,et al.Proteomics change of heat-treated milk by two-dimensional electrophoresis technology[J].China Dairy Industry,2016,44(3):23-26.(in Chinese)
[13] HERNANDEZ-CASTELLANO L E,ALMEIDA A M,VENTOSA M,et al.The effect of colostrum intake on blood plasma proteome profile in newborn lambs:Low abundance protein[J].BMC Veterinary Research,2014,10:85-92.
[14] PISANU S,GHISAURA S,PAGNOZZI D,et al.Characterization of sheep milk fat globule proteins by two-dimensional polyacrylamide gel electrophoresis/mass spectrometry and generation of a reference map[J].International Dairy Journal,2012,24(2):78-86.
[15] 杨永新,王加启,卜登攀,等.奶牛初乳与常乳乳蛋白变化的比较蛋白质组学研究[J].中国农业大学学报,2010,15(2):47-52. YANG Y X,WANG J Q,BU D P,et al.Developmental changes of the milk protein from colostrums to milk in the periparturient dairy cattle[J].Journal of China Agricultural University,2010,15(2):47-52.(in Chinese)
[16] GULLIKSEN S M,LIE K I,SØLVERØD L,et al.Risk factors associated with colostrum quality in Norwegian dairy cows[J].Journal of Dairy Science,2008,91(2):704-712.
[17] 陈文亮,苏米亚,贾宏信,等.α-乳白蛋白的功能特性及其在婴儿配方乳粉中的应用[J].乳业科学与技术,2019,42(1):33-38. CHEN W L,SU M Y,JIA H X,et al.Functional characteristics and application in infant formula of alpha-lactalbumin[J].Journal of Dairy Science and Technology,2019,42(1):33-38.(in Chinese)
[18] PERMYAKOV E P,BERLINER L J.α-lactalbumin:Structure and function[J].FEBS Letters,2000,473(3):269-274.
[19] KONTOPIDIS G,HOLT C,SAWYER L.Invited review:β-lactoglobulin:Binging properties,structure,and function[J].Journal of Dairy Science,2004,87(4):785-796.
[20] HERNANDEZ-LEDESMA B,RECIO I,AMIGO L.β-lactoglobulin as source of bioactive peptides[J].Amino Acids,2008,35(2):257-265.
[21] HEJTMANKOVA A,PIVEC V,TRNKOVA E,et al.Differences in the composition of total and whey proteins in goat and ewe milk and their changes throughout the lactation period[J].Czech Journal of Animal Science,2012,57(7):323-331.
[22] 白文林,郑玉才,王杰,等.中国荷斯坦牛牛乳生化组成及乳蛋白遗传多样性[J].四川畜牧兽医,2002,29(3):23-24. BAI W L,ZHENG Y C,WANG J,et al.Composition and genetic polymorphism of milk protein of Chinese-Holstein[J].Sichuan Animal & Veterinary Sciences,2002,29(3):23-24.(in Chinese)
[23] RECIO I,PEREZ-RODRIGUEZ M L,AMIGO L,et al.Study of the polymorphism of caprine milk caseins by capillary electrophoresis[J].Journal of Dairy Research,1997,64(4):515-523.
[24] AGANGA A A,AMARTEIFIO J O,NKILE N.Effect of stage of lactation on nutrient composition of Tswana sheep and goat's milk[J].Journal of Food Composition & Analysis,2002,15(5):533-543.
[25] CEBALLOS L S,MORALES E R,ADARVE G T,et al.Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology[J].Journal of Food Composition and Analysis,2009,22(4):322-329.
[26] 张娜娜.妊娠后期饲粮蛋白水平对湖羊羔羊生长和初乳蛋白质组的影响[D].兰州:兰州大学,2018. ZHANG N N.Effects of dietary protein level during late gestation on growth of Hu lambs and colostrum proteome[D].Lanzhou:Lanzhou University,2018.(in Chinese)

Differential Proteomic Study of Colostrum and Mature Milk of Hu Sheep Based on Two-dimensional Electrophoresis Technology

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[2]	WU Tao, QI Yunxia, LIU Wei, ZHU Honglong, HUANG Dongwei, DING Haisheng, ZHAO Huiling, CHENG Guanglong, ZHAO Xiaowei. Effect of Colostrum and Milk Feeding on Hepatic Proteins of Calves Based on Proteomics Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 37-45.
[3]	AN Xiaopeng, LIU Nan, HU Zhengjiamei, HU Jincao, XU Xiaolong, ZHANG Lei, SONG Yuxuan. Comparative Analysis of Lactation Performance of East Friesen Sheep, Hu Sheep and East Friesen-Hu Hybrid Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 238-245.
[4]	YANG Chaohui, MA Xuelian, WANG Fan, YI Pengfei, WU Jing, FENG Mengdi, CHEN Yuzhu, SUN Yawei, WUMAIERJIANG·Yahefu, WANG Sheng, XU Jianbiao, ZHONG Qi, ZHOU Zhenyong, YAO Gang. Determination of Colostrum Yield and Quality of Angus Cows and Evaluation of Passive Immunity Effect of Newborn Calves [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3353-3361.
[5]	LIU Gang, ZHU Fangxian, ZHANG Xiaowei, GU Feng, LIU Lijun, SU Rui, MENG Fei, XU Haitao, SUN Feizhou. Evaluation of Embryo and Sperm Cryopreservation in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3492-3499.
[6]	HE Zhanxing, HUANG Meifen, CHENG Yumei, ZHANG Quanpeng, ZHANG Jicai, YANG Kai, WANG Ankui, ZHOU Yaping, HUANG Bizhi. Comparison of Colostrum Physicochemical Characterization Between Dulong Cattle and Yunling Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2156-2164.
[7]	ZHANG Rui, ZHOU Weiqiang, MA Yue, WU Jianping, LEI Zhaomin. Effects of Oregano Essential Oil and Colostrum Complex on Growth Performance, Serum Indicators Related Antioxidant and Immune in Opportunity Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1314-1321.
[8]	WANG Jiang, LIU Zhiqiang, SUN Hongchen, HE Xinyu, NUHA Maidan, QIU Hongjun, WANG Yan, YIMING Sulaiman. Analysis of Influencing Factors of Birth Weight Traits and Estimation of Heritability in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3846-3855.
[9]	NING Jingyang, MA Longgang, CHEN Ziwei, AN Tao, CHANG Yao, WANG Kai, WANG Lei, CHEN Shaokan, ZHANG Fan, GUO Gang, ZHANG Hailiang, WANG Yachun. Population Characteristic of Dry Period Length and Its Relationship with Production Performance in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3368-3377.
[10]	DAI Guodong, YAN Hongbin, LI Li, FU Baoquan, JIA Wanzhong. Research Progress on Screening Methods for Candidate Vaccine Molecules Against Parasitic Zoonoses [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2177-2187.
[11]	YANG Jiangfeng, BAI Yuanyuan, ZHAO Xiaokun, YU Xiaojie, LI Zhanli, ZHANG Lei, WANG Jing. Effects of Supplementary Feeding Time by Fence on Growth Performance, Wool Quality and Serum Biochemical Indices of Dorper×Hu Sheep Hybrid Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3644-3651.
[12]	HE Dongqian, TAO Jinzhong, CHEN Liyao, WANG Junkui. Research Progress of Sheep Hair Follicle and Keratin [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 248-256.
[13]	LIU Haixia, HAN Dayong, ZHU Aiwen, YAN Wei, WANG Buzhong, CAI Zijian. Effect of the Polymorphism of GnRHR and INHA Genes and Its Interaction on Litter Size in Wuhu Hybrid Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 780-788.
[14]	NIU Chunyu, SHI Linlin, SUN Miao, LIU Yanmin, HUANG Shaolei, CHENG Chao, LI Peifeng. Applications of Omics Approaches in Isolation and Identification of Lactic Acid Bacteria and It’s Functional Research [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3882-3889.
[15]	ZHAO Zhida, WANG Xinyue, SHI Tianpei, HU Wenping, SHANG Mingyu, ZHANG Li. Identification of Hu Sheep Family Based on Microsatellite Markers [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3573-3583.