基于微卫星标记鉴定湖羊家系

doi:10.16431/j.cnki.1671-7236.2020.11.019

Abstract

Abstract: The purpose of this study was to explore identification and division methods of Hu sheep family based on simple sequence repeats(SSR) polymorphism.Nine SSR loci of BM3051,HH64,TGLA137,MAF33,FCB48,VH72,INRA023,ETH152 and MCM527 were selected,which were distributed in several autosomes,with high polymorphism and high heterozygosity.DNA was extracted from the blood samples of 30 Hu breeding rams by blood DNA extraction kit.SSR primers were synthesized and labeled with the fluorescent group.The products were amplified by touch-down PCR.After genotyping,CERVUS3.0.7 was used for genotyping analysis.POPGENE1.32 was used to construct a dendrogram based on Nei's genetic distance to divide Hu sheep families.The results showed that the cumulative exclusion probability of single parent was 99.2094% when the genotypes of both parents were unknown,99.9688% when a single parent genotypes were known,99.9999% when both parents' genotypes were unknown.Exclusion probability could meet the requirements of paternity identification and pedigree analysis in genetic breeding.UPGMA clustering map was constructed according to Nei's genetic distance,and 30 breeding rams were divided into six families,which provided a reference for sheep breeding.The method could run the family identification and division of Hu sheep,which had application value for the breeding of Hu sheep.

Key words: Hu sheep; microsatellite marker; touch-down PCR; pedigree identification

CLC Number:

S816.6

ZHAO Zhida, WANG Xinyue, SHI Tianpei, HU Wenping, SHANG Mingyu, ZHANG Li. Identification of Hu Sheep Family Based on Microsatellite Markers[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3573-3583.

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Identification of Hu Sheep Family Based on Microsatellite Markers

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