利用微卫星标记分析伊犁鹅的保种效果

doi:10.16431/j.cnki.1671-7236.2019.06.022

Abstract

Abstract:

This study was aimed to analyze the conservation efficiency of Yili goose in reservation farm,and 9 microsatellite markers were used to detect the genetic diversity of three generations (0,1 and 2 generations) of Xinjiang Yili goose.In this experiment,all blood samples were took from three generations of Yili goose.DNA was extracted using phenol-chloroform extraction method,and detected by 12% polyacrylamide gel electrophoresis,silver nitrate staining and judge genotype.The results showed that a total of 78 alleles were detected in 9 microsatellite loci in three generations of Yili goose,26 alleles were detected in 0,1 and 2 generations.The average expected heterozygosity (He) of three generations were 0.4322,0.4232 and 0.4516,respectively.The average polymorphic information content (PIC) were 0.3751,0.3707 and 0.3936,respectively,it showed that three generations of Yili goose were in moderate polymorphism,the genetic variation was at a medium level,and there was no significant difference in average He and PIC among three generations (P>0.05).The genetic distances of three generations were 0.0097,0.0184 and 0.0074,respectively.In conclusion,Yili goose had rich genetic diversity,and the current conservation method adopted by the conservation farm had better preserved the original genetic diversity of Yili goose.

Key words: Yili goose; microsatellite markers; conservation

CLC Number:

S813.3

WU Yingping, GAO Feng, PENG Xiao, WANG Junhua, MI Sijia, SUN Miao, LIU Xiaoxiao, LI Haiying, LU Lizhi. Analysis of Conservation Efficiency of Yili Goose by Microsatellite Markers[J]. , 2019, 46(6): 1746-1755.

References

[1] 曾凡明,那·杜干.伊犁鹅的品种特性及草原放牧饲养管理技术[J].中国动物保健,2015,12:6-8. ZENG F M,NA D G.Characteristics and management techniques of grassland grazing in Yili goose[J].Chinese Journal of Animal Health,2015,12:6-8.(in Chinese)
[2] POWELL W,MORGANTE M,ANDRE C,et al.The comparision of RFLP,RAPD,AFLP and SSR markers for gemplasm analysis[J]. Molecular Breeding,1996,2:225-238.
[3] 刘雅丽,章晓伟,马敏彪,等.利用微卫星标记检测仙居鸡保种效果[J].中国家禽,2016,38(22):67-70. LIU Y L,ZHANG X W,MA M B,et al.Detection of the effect of Xianju chicken preservation by microsatellite markers[J].China Poultry,2016,38(22):67-70.(in Chinese)
[4] 徐小钦,陈黎,李进军,等.微卫星标记技术鉴定某保种场四川白鹅保种效果[J].浙江农业学报,2015,27(5):751-755. XU X Q,CHEN L,LI J J,et al.Conservation efficiency of Sichuan White goose in a conservation farm analyzed by microsatellite markers[J].Acta Agriculturae Zhejiangensis,2015,27(5):751-755.(in Chinese)
[5] 萨姆布鲁克J,等著.金冬雁,等译.《分子克隆实验指南》[M].第2版.北京:科学出版社,1999. SAMBROOK J,et al(editor).JIN D Y,et al(translator).Molecular cloning:A Laboratory Manual[M].2nd Edition.Beijing:Science Press,1999.(in Chinese)
[6] 马杰.太湖鹅生长性状与微卫星DNA标记的相关性分析[D].南京:南京农业大学,2006. MA J.Correlation analysis of growth trait and micros atellite DNA markers in Taihu goose[D].Nanjing:Nanjing Agricultural University,2006.(in Chinese)
[7] 任战军,宋旭旭,王永絮,等.利用微卫星标记分析3个鹅品种的遗传多样性[J].西北农业学报,2011,20(2):40-43. REN Z J,SONG X X,WANG Y X,et al.Genetic diversity of three goose breeds analyzed by microsatellite[J].Acta Agriculturae Boreali-Occidentalis Sinica,2011,20(2):40-43.(in Chinese)
[8] LI J J,LI G Q,SHEN J D,et al.Characterisation of 57 novel microsatellite markers from the goose (Anser cygnoides)[J].Journal of Applied Animal Research,2013,41(1):111-116.
[9] WEIß B M,POGGEMAM K,OLEK K,et al.Isolation and characterization of microsatellite marker loci in the Greylag goose (Anser anser)[J].Molecular Ecology Resources,2008,8(6):1411-1413.
[10] NOREIKIENE K,TEACHER A G F,MADSEN J,et al.Isolation and characterization of 55 novel microsatellite markers for the pink-footed goose (Anser brachyrhynchus)[J].Conservation Genetics Resources,2012,4(2):423-428.
[11] CATHEY J C,DEWOODY J A,SMITH L M.Microsatellite markers in Canada geese (Branta canadensis)[J].Journal of Heredity,1998,89(2):173-175.
[12] 杜文兴,虞德兵,刘红林,等.双重抑制PCR技术分离鹅微卫星标记[J].南京农业大学学报,2005,28(3):63-67. DU W X,YU D B,LIU H L,et al.Isolation of goose microsatellite markers using dual-suppression-PCR technique[J].Journal of Nanjing Agricultural University,2005,28(3):63-67.(in Chinese)
[13] 夏婷婷,周丽,陈兴勇,等.14个微卫星标记分析巢湖麻鸭群体的遗传多样性[J].福建农林大学学报(自然科学版),2015,44(3):293-297. XIA T T,ZHOU L,CHEN X Y,et al.Population genetic diversity analysis of Chaohu duck using fourteen microsatellite markers[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2015,44(3):293-297.(in Chinese)
[14] 黄勋和,张金枫,陈洁波,等.贵妃鸡MHC-B区域微卫星位点LEI0258遗传多样性研究[J].中国畜牧兽医,2016,43(12):3300-3305. HUANG X H,ZHANG J F,CHEN J B,et al.Genetic diversity of microsatellite LEI0258 in MHC-B region of Royal chicken[J].China Animal Husbandry & Veterinary Medicine,2016,43(12):3300-3305.(in Chinese)
[15] 白俊艳,庞有志,杨又兵,等.黑羽鹌鹑的EST-SSR标记开发与应用[J].中国畜牧兽医,2016,43(5):1323-1328. BAI J Y,PANG Y Z,YANG Y B,et al.Development and application of EST-SSR markers of Black quail[J].China Animal Husbandry & Veterinary Medicine,2016,43(5):1323-1328.(in Chinese)
[16] 伍革民,张继,唐继高.4个贵州乌骨鸡群体遗传关系的微卫星标记分析[J].江苏农业科学,2018,46(21):169-171. WU G M,ZHANG J,TANG J G,et al.Analysis of genetic relationships among four Black-bone chicken populations in Guizhou province using microsatellite marks[J].Jiangsu Agricultural Sciences,2018,46(21):169-171.(in Chinese)
[17] 方倩倩,刘雅丽,李祥龙,等.利用微卫星标记技术分析太湖鹅保种效果[J].畜牧与兽医,2016,48(12):19-24. FANG Q Q,LIU Y L,LI X L,et al.Analysis on genetic diversity and conserved effect of the Taihu goose using microsatellite markers[J].Animal Husbandry & Veterinary Medicine,2016,48(12):19-24.(in Chinese)
[18] 季华员,黄江南,李海琴,等.兴国灰鹅微卫星标记的遗传多样性分析[J].畜牧与兽医,2017,49(8):6-9. JI H Y,HUANG J N,LI H Q,et al.Genetic diversity analysis of Xingguo gray goose based on microsatellite markers[J].Animal Husbandry & Veterinary Medicine,2017,49(8):6-9.(in Chinese)
[19] 左瑞华.皖西白鹅保种群动态监测及遗传变异的研究[D].合肥:安徽农业大学,2012. ZUO R H.Dynamic monitoring of Wanxi White goose core conservation population and its genetic variation[D].Hefei:Anhui Agricultural University,2012.(in Chinese)
[20] 宋旭旭.利用微卫星标记分析3个鹅品种的遗传多样性[D].杨凌:西北农林大学,2010. SONG X X.Genetic diversity of three goose breeds by microsatellite markers[D].Yangling:Northwest A & F University,2010.(in Chinese)
[21] 张雪娜,陈燏,郗正林,等.丽佳鹅、五龙鹅和长白Ⅰ型鹅遗传多样性的微卫星标记分析[J].江苏农业科学,2008,1:161-165. ZHANG X N,CHEN Y,XI Z L,et al.Microsatellite marker analysis of genetic diversity of Lijia goose,Wulong goose and Changbai goose[J].Jiangsu Agricultural Sciences,200,1:161-165.(in Chinese)
[22] 郭丹,韩迪,王春艳,等.辽宁绒山羊7个微卫星位点的遗传多样性分析[J].中国畜牧兽医,2010,37(12):99-103. GUO D,HAN D,WANG C Y,et al.Genetic diversity of seven microsatellite loci in Liaoning cashmere goat[J].China Animal Husbandry & Veterinary Medicine,2010,37(12):99-103.(in Chinese)
[23] 屠云洁,陈宽维,汤青萍,等.江西4个地方鹅品种遗传多样性的微卫星标记分析[J].扬州大学学报(农业与生命科学版),2006,27(1):33-37. TU Y J,CHEN K W,TANG Q P,et al.Genetic diversity of Chinese 4 indigenous goose breeds in Jiangxi province based on microsatellite markers[J].Journal of Yangzhou University (Agricultural and Life Science Edition),2006,27(1):33-37.(in Chinese)
[24] VANHALA T,TUISKULA-HAAVISTO M,ELO K,et al.Evaluation of genetic distances between eight chicken lines using microsatellite markers[J].Poultry Science,1998,77(6):783-790.
[25] 赵东伟,卢建,李春苗,等.徐海鸡保种群体遗传多样性及保种效果分析[J].中国家禽,2017,39(21):59-61. ZHAO D W,LU J,LI C M,et al.Genetic diversity and conservation effect of Xuhai chicken conservation population[J].China Poultry,2017,39(21):59-61.(in Chinese)
[26] 张跟喜,丁馥香,王金玉,等.微卫星标记分析边鸡遗传多样性及保种效果[J].农业生物技术学报,2010,18(5):944-950. ZHANG G X,DING F X,WANG J Y,et al.Analysis on genetic diversity and conserved effect of Bian chicken using microsatellite markers[J].Journal of Agricultural Biotechnology,2010,18(5):944-950.(in Chinese)
[27] 乔娜.我国9个地方鹅品种遗传多样性分析及异地保种效果的监测[D].扬州:扬州大学,2010. QIAO N.Analysis of genetic diversity of 9 Chinese indigenous domestic geese based and effect monitoring of allopatry conservation[D].Yangzhou:Yangzhou University,2010.(in Chinese)

Analysis of Conservation Efficiency of Yili Goose by Microsatellite Markers

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