早胜牛微卫星多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2019.07.012

Abstract

Abstract:

To better understand the genetic information and provide theoretical basis for the breeding and conservation of Zaosheng cattle,13 pairs of microsatellite primers were used to analyze the genetic diversity by PCR amplification and capillary electrophoresis in 48 Zaosheng cattle.The correlation between different genotypes at 13 microsatellite loci and seven traits (birth weight,6-month-old weight,adult weight,body height,body length,chest circumference and cannon circumference) of Zaosheng cattle were analyzed.The results showed that the average number of alleles at 13 microsatellite polymorphic loci was 9.54,the average effective allele was 4.792,the average observed heterozygosity was 0.498,the average expected heterozygosity was 0.783,and the average polymorphic information content was 0.712.The correlation analysis results showed that D12S4 and D15S10 were correlated with birth weight;D18S5 and D12S4 were correlated with 6-month-old weight;D14S31 and D11S15 were correlated with adult weight;D18S5,D15S10 and D19S2 were correlated with body height;D15S10,D14S31 and D19S2 were correlated with body length;D18S5,D12S4 and D19S2 were correlated with chest circumference;D18S5 and D19S2 were correlated with cannon circumference.This results found that 6 loci (D18S5,D12S4,D15S10,D14S31,D19S2 and D11S15) were associated with the traits of Zaosheng cattle among the selected 13 microsatellite loci.The microsatellite loci selected in this experiment had rich polymorphisms in Zaosheng cattle population,and were associated with economic traits,which could be used for genetic resources evaluation and early breeding improvement of Zaosheng cattle.

Key words: microsatellite markers; Zaosheng cattle; polymorphism; genetic markers

CLC Number:

S823.9⁺4

CHI Haobin, HAN Xiangmin, LANG Xia, WANG Cailian. Analysis of Microsatellite Polymorphism and Its Association with Growth Traits of Zaosheng Cattle[J]. , 2019, 46(7): 1976-1985.

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Analysis of Microsatellite Polymorphism and Its Association with Growth Traits of Zaosheng Cattle

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