10个云南地方猪种微卫星遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2018.04.020

Abstract

Abstract:

Baoshan pig,Diqing Tibetan pig,Gaoli Gongshan pig,Lijiang pig,Mingguang Small-ear pig,Diannan Small-ear pig,Saba pig,Dahe pig,Zhaotong pig and Dahe Black pig are ten local pig breeds with excellent breed characteristics in Yunnan province,China.To assess their genetic diversity and genetic structure,a total of 549 unrelated ear tissue samples from the ten indigenous pig breeds were genotyped using 15 fluorescence-labeled microsatellite DNA markers of 14 chromosomes in the ArkDB database.The number of alleles (Na),effective allele number (Ne),Shannon's information index (I),apparent heterozygosity (Ho),expected heterozygosity (He), F-statistics(Fis,Fit,Fst),gene flow (Nm),population genetic distance and other related parameters and polymorphic information content (PIC) were determined,cluster tree was built by NTsys 2.10,and STRUCTRUE 2.3.3 software was used to evaluate the genetic structure of the population.The results showed that the Na of 15 microsatellite loci was 293,ranging from 5 to 38 alleles per locus.The mean number of alleles and effective number of alleles per locus were found to be 19 and 8.3682,respectively.The average Shannon's information index,observed heterozygosity,expected heterozygosity and polymorphism information content of 10 indigenous pig populations ranged from 1.4374 to 2.0317,0.6389 to 0.7756,0.7021 to 0.8281,0.6647 to 0.7993,respectively.The average inbreeding coefficient within accessions (Fis) and overall inbreeding coefficient (Fit) per locus ranged from -0.0678 to 0.4594 and 0.0618 to 0.5567,respectively.The moderate degree of genetic differentiation index (Fst) ranged from 0.0713 to 0.1801 with an average of 0.1101,which demonstrated that approximately 11.01% of genetic variations were among populations and 88.99% of genetic differences were within populations.The high level of gene flow at each locus was discovered from 1.6392 to 3.2551,with a mean Nm of 2.0214.The phylogenetic relationships of pig breeds were constructed using UPGMA method based on Nei's genetic distance.The UPGMA dendrogram showed that the Gaoli Gongshan pig and Diqing Tibetan pig jointed as one cluster.And then joined with Baoshan pig,Lijiang pig and Mingguang Small-ear pig breeds as a big group.STRUCTURE analysis confirmed the analysis result observed in the UPGMA tree.The study indicated that the genetic diversity was abundant in ten Yunnan local pig breeds.There were close inbreeding phenomenon in the ten breeds.The level of genetic differentiation among populations was moderately,with a certain amount of gene flow.

Key words: Yunnan local pig breeds; microsatellite markers; genetic diversity; genetic structure

CLC Number:

S813

OUYANG Yina, JIANG Yanting, SUN Limin, YUAN Yueyun, LI Dongjiang, LIANG Jiachong, HONG Qionghua. Genetic Diversity Analysis of Ten Yunnan Local Pig Breeds Using Microsatellite DNA Markers[J]. , 2018, 45(4): 992-1001.

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Genetic Diversity Analysis of Ten Yunnan Local Pig Breeds Using Microsatellite DNA Markers

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