大白猪BMPR-ⅠB基因多态性及其与繁殖性状的相关性研究

doi:10.16431/j.cnki.1671-7236.2018.04.019

Abstract

Abstract:

This study was aimed to investigate the polymorphism of bone morphogenetic protein receptor-ⅠB (BMPR-ⅠB) gene and its correlation with the reproductive traits in Large White pigs.The polymorphism of BMPR-ⅠB gene was analyzed by high-throughput sequencing technologiy,the association of different genotypes of BMPR-ⅠB gene with the total number of births,alive births,deaths and deformities using the least square method by SPSS statistical software.The results showed that the polymorphism was not detected in BMPR-ⅠB gene 746A > G and 852G > A loci,in the 804G > C locus,the frequency of GG genotype was dominant genotype,and G was the dominant allele; In the 960C > T locus,the frequency of genotypes CC,TT and CT were higher in American,British and Canadian Large White pigs,respectively.The results of χ² test showed that the 804G > C and 960C > T loci were fit with Hardy-Weinberg equilibrium in the different Large White pigs lines (P > 0.05).There were no significant differences in the effects of 804G > C locus on the number of total births,alive births,still births,deformity and other reproductive traits of the three different lines of Large White pigs (P > 0.05).The number of stillbirths with TT genotype in American Large White pigs were significantly lower than that of CC genotype in 960C > T locus (P < 0.05),which was decreased by 1.027; The total litter size of the British Large White pigs with TT genotype was significantly higher than that of CC genotype (P < 0.05),and extremely significantly higher than that of CT genotype (P < 0.01),which was in-creased 1.976 and 2.118,respectively; The number of live births in Canadian Large White pigs with TT genotype was significantly higher than that of CC genotype (P < 0.05),the stillbirth and deformity of TT genotype were significantly lower than that of CC genotype (P < 0.05),which were decreased 0.707 and 0.337,respectively.The TT genotype in 960C > T locus could be used as the marker genotype for reproductive traits of three different lines of Large White pigs.

Key words: Large White pigs; BMPR-ⅠB gene; reproductive traits; correlation analysis

CLC Number:

S813.3

HU Huiyan, JIA Qing, HOU Shengkui, LIU Jin, LIU Yangyang, ZHANG Weifeng, ZHAO Kunfeng, WANG Guijiang. Study on Polymorphism of BMPR-ⅠB Gene and Its Correlation with Reproductive Traits in Large White Pig[J]. , 2018, 45(4): 984-991.

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Study on Polymorphism of BMPR-ⅠB Gene and Its Correlation with Reproductive Traits in Large White Pig

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