大白猪ENO3基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.01.020

Abstract

Abstract: [Objective] The aim of this study was to explore the single nucleotide polymorphism (SNP) of enolase 3 (ENO3) gene, and analyze the linkage imbalance between SNPs and its correlation with growth traits in Yorkshire pigs. [Method] A total of 316 sows were selected, the SNP of ENO3 gene were identified by PCR amplification product sequencing of mixed DNA samples. GenoPlexs typing technique was used to genotype the target SNP of each individual, the linkage disequilibrium and correlation analysis were conducted with daily gain, days, backfat thickness, loin-eye area and loin-eye thickness at 100 kg body weight, and explored the correlation between ENO3 gene SNP and growth traits of Yorkshire pigs. [Result] The results showed that 9 SNPs were identified, among which rs196953768 was completely linked to rs324943047, rs345530479 and rs329283992 (D'=1, r²=1), and strongly linked to rs342598032 (D'=1, r²=0.99), rs327882211 was strongly linked with rs341541240 and rs325279236 (D'=1, r²>0.7). ENO3 gene rs196953768 and its complete linkage locus were significantly correlated with days and daily gain at 100 kg body weight (P<0.05), rs327882211 and rs341541240 were significantly correlated with loin-eye area and loin-eye thickness at 100 kg body weight (P<0.05);rs325279236 was significantly correlated with loin-eye area at 100 kg body weight (P<0.05);rs786427749 and rs342598032 had no significant correlation with the above growth traits (P>0.05). [Conclusion] In this study, a total of 9 SNPs were excavated, 4 fully linked SNPs were identified on ENO3 gene, 7 SNPs significantly correlated with growth traits were screened, it provided reference data for molecular marker assisted breeding of Yorkshire pigs.

Key words: Yorkshire pigs; ENO3 gene; SNP; growth traits; linkage disequilibrium analysis; correlation analysis

CLC Number:

S813.3

ZHAO Yuqiang, CHEN Xin, LI Qingchun, MA Jilin, DONG Yinhe, WANG Deming, GUO Lingyun, HUANG Tao. Polymorphism of ENO3 Gene and Its Correlation with Growth Traits in Yorkshire Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 188-196.

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Polymorphism of ENO3 Gene and Its Correlation with Growth Traits in Yorkshire Pigs

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