松辽黑猪NR5A2基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.09.027

Abstract

Abstract: This study was aimed to investigate the association of the polymorphism of NR5A2 gene with reproductive traits in Songliao Black pigs. 130 Songliao Black pigs were selected as the research objects. The SNP of 6 exons of NR5A2 gene were found by PCR sequencing. The polymorphism of SNPs was analyzed by HRM typing technology. The correlation between SNPs of NR5A2 gene and total number born, number born alive, birth weight, 3-week weight, weaning weight, weaning piglet number and nipple number was analyzed by SPSS 19.0 software. The results showed that there was an SNP (C99T) in exon 6 of NR5A2 gene in Songliao Black pig, and three genotypes were detected:CC, CT and TT. The results of genetic parameter analysis and Chi square test showed that Songliao Black pig population was in Hardy-Weinberg equilibrium (P>0.05), but the heterozygosity of C/T mutation site was relatively low, and had a low variation in Songliao Black pig population, which belonged to moderate polymorphism (0.25<PIC<0.5). In the C99T site of NR5A2 gene, the total number born, number born alive and weaning piglets of CC genotype were the highest, and significantly higher than TT genotype (P<0.05), the number born alive and weaning piglet number were also significantly higher than CT genotype (P<0.05). There was no significant difference in birth weight, 3-week weight, weaning weight and nipple number among different genotypes (P>0.05). In conclusion, the mutation site C99T in exon 6 of NR5A2 gene was significantly associated with number born of Songliao Black pigs. However, whether this mutation site could be used as a genetic marker of number born of Songliao Black pigs needed further study in a larger population.

Key words: Songliao Black pigs; NR5A2 gene; reproductive traits; association analysis; genetic marker

CLC Number:

S813.3

ZHANG Yunpeng, LIU Qingyu, ZHANG Qi, GAO Yi, ZHANG Qing, ZHANG Jingbo, ZHANG Shumin. Polymorphism of NR5A2 Gene and Its Association with Reproductive Traits in Songliao Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3361-3367.

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Polymorphism of NR5A2 Gene and Its Association with Reproductive Traits in Songliao Black Pigs

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