二甲双胍对牛骨骼肌卫星细胞增殖与分化的影响

doi:10.16431/j.cnki.1671-7236.2021.09.026

Abstract

Abstract: In order to explore the effects of metformin on the proliferation and differentiation of bovine skeletal muscle satellite cells, bovine skeletal muscle satellite cells cultured in vitro were treated with 0 (control group), 1, 2 and 4 mmol/L metformin, and using CCK-8 method to screen the optimal concentration of metformin on bovine skeletal muscle satellite cells. The effect of metformin treatment on the proliferation of bovine skeletal muscle satellite cells was detected by EdU staining method, and the metformin-treated bovine skeletal muscle satellite cells were myogenically induced and differentiated in vitro, and the cell status of bovine skeletal muscle satellite cells during the differentiation period was observed. Then Western blotting was used to detect the expression of the differentiation markers myosin heavy chain (MyHC) and myogenin (MyoG) of bovine skeletal muscle satellite cells at 24, 48 and 72 h of differentiation. The results showed that the optimal concentration of metformin on bovine skeletal muscle satellite cells was 2 mmol/L. After treating bovine skeletal muscle satellite cells with 2 mmol/L metformin, the cell proliferation rate were significantly reduced (P<0.05), indicating that metformin could inhibit the proliferation of bovine skeletal muscle satellite cells. The number and diameter of myotubes formed by bovine skeletal muscle satellite cells after induced differentiation showed a decreasing trend, and the expressions of myogenic differentiation markers MyHC and MyoG of bovine skeletal muscle satellite cells were significantly lower than 0 mmol/L (control) group (P<0.05) at 24, 48 and 72 h after differentiation, indicating that 2 mmol/L metformin could inhibit the myogenic differentiation process of bovine skeletal muscle satellite cells. The results of this study showed that metformin could significantly inhibit the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells, providing a theoretical basis for the application of metformin in muscle development regulation and muscle injury repair.

Key words: metformin; bovine skeletal muscle satellite cells; cell proliferation; cell differentiation

CLC Number:

S829.9

LIU Qiuhua, ZHU Feifei, WANG Yimin, ZHANG Linlin, LI Xin, GUO Yiwen, GUO Hong, DING Xiangbin. Effects of Metformin on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3354-3360.

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Effects of Metformin on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells

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