基于AMPK通路研究葛根素对猪前体脂肪细胞成脂分化的影响

doi:10.16431/j.cnki.1671-7236.2022.06.009

Abstract

Abstract: 【Objective】 This study was aimed to explore whether puerarin could promote the differentiation of Songliao Black pig precursor adipocytes through the AMPK pathway, in order to provide a reference for adding puerarin to diet to improve pork meat quality.【Method】 When the confluence degree of precursor adipocytes of Songliao Black pig reached 90%, the differentiation was induced for 4 days.When inducing differentiation, the cells were divided into control group (Con), puerarin group (Pue), puerarin+AMPK activator AICAR group (AICAR) and puerarin+AMPK inhibitor CompoundC group (CompoundC).Puerarin was not added to the induction solution in the control group, while 40 μmol/L puerarin was added in the Pue group, 40 μmol/L puerarin +500 μmol/L AICAR were added in AICAR group, 40 μmol/L puerarin +20 μmol/L CompoundC were added in CompoundC group.After induction and differentiation, the cells of each group were collected and the triglyceride (TG)concentration was detected by triglyceride enzyme method, the expression levels of AMPK subunits and adipogenic differentiation related genes were detected by Real-time quantitative PCR, peroxisome proliferator activated receptor γ (PPARγ) AMP-dependent protein kinase (AMPK), p-AMPK (Thr-172), acetyl-CoA carboxylase (ACC) were detected by Western blotting.Autodock Vina 1.20 was used to predict the molecular docking of puerarin and AMPKα subunits.【Result】 The results of triglyceride determination showed that compared with Con group, the triglyceride concentration of Pue group was significantly up-regulated (P<0.05);Compared with Pue group, the triglyceride concentration of AICAR group was significantly decreased (P<0.05).The results of Real-time quantitative PCR showed that compared with Con group, the expressions of PPKAG2, PPKAB1, PPKAB2, PPKAA1 and PGC-1α in Pue group were significantly decreased (P<0.05), the expressions of C/EBPα, PPARγ and ACC in Pue group were significantly increased (P<0.05);Compared with Pue group, the expressions of PPKAG1, PPKAG2, PPKAB1, PPKAB2 and PPKAA1 in AICAR group were significantly increased (P<0.05), the expressions of C/EBPα, PPARγ and ACC in AICAR group were significantly decreased (P<0.05), the expressions of PPKAG1, PPKAG2 and PPKAB2 in CompoundC group were significantly decreased (P<0.05), the expressions of C/EBPα and ACC in CompoundC group were significantly increased (P<0.05).Western blotting results showed that compared with Con group, the expressions of PPARγ and ACC protein in Pue group were significantly up-regulated(P<0.05), the expressions of AMPK, p-AMPK protein were significantly down-regulated (P<0.05), and p-AMPK/AMPK was significantly decreased (P<0.05);Compared with Pue group, the expressions of PPARγ and ACC protein in AICAR group were significantly decreased (P<0.05), while AMPK and p-AMPK protein were significantly up-regulated (P<0.05), the expressions of PPARγ and ACC protein in CompoundC group were significantly increased (P<0.05), while the expressions of AMPK, p-AMPK protein and p-AMPK/AMPK were significantly decreased (P<0.05).【Conclusion】 40 μmol/L puerarin could significantly increase the content of triglycerides in adipocytes and significantly up-regulate the expressions of the adipogenic differentiation genes PPARγ, C/EBPα and ACC, significantly down-regulate the expressions of AMPK subunits.AMPK activator AIRCR could significantly inhibit the effect of puerarin, indicating that puerarin could regulate the adipogenic differentiation of Songliao Black pig precursor adipocytes by inhibiting AMPK pathway.

Key words: puerarin; Songliao Black pigs; AMPK pathway; adipogenic differentiation; adipocyte

CLC Number:

S828

LYU Xiangzhou, ZHANG Qi, LI Xin, YU Yongsheng. Effect of Puerarin on Adipogenic Differentiation of Porcine Preadipocytes Based on AMPK Pathway[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2088-2097.

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Effect of Puerarin on Adipogenic Differentiation of Porcine Preadipocytes Based on AMPK Pathway

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