秦川牛ACTC1基因的表达特性分析

doi:10.16431/j.cnki.1671-7236.2020.08.003

Abstract

Abstract: The study was aimed to reveal the temporal and spatial expression of ACTC1 gene in Qinchuan cattle,which will lay the foundation for further research on the functions of ACTC1 gene in muscle development and fat deposition of beef cattle.Real-time quantitative PCR was used to detect the expression of ACTC1 gene in 13 tissues of 24-month-old adult Qinchuan cattle and 12 kinds of tissues of 4-day-old Qinchuan cattle.At the same time,the expression characteristics of this gene in myoblasts and preadipocytes of Qinchuan cattle at different differentiation stages (0,2,4,6 and 8 d) were studied and analyzed.The results showed that the expression of ACTC1 gene was the highest in heart of Qinchuan cattle,followed by skeletal muscle.Except for rumen and kidney,the expression of ACTC1 at 24-month-old adult cattle was significantly or extremely significantly higher than that at 4-day-old newborn cattle (P<0.05;P<0.01).The expression of ACTC1 gene in myoblasts increased at first and then decreased along with the myoblasts differentiation.On the 2nd,4th,6th and 8th day of myoblast differentiation,the expression of ACTC1 gene was significantly different from that on 0th day (P<0.01),which were 2.6,4.7,5.6,and 4.2 times of 0th day,respectively.This was consistent with the differentiation rate of myoblasts.The expression trend of ACTC1 gene in adipocytes decreased at first and then increased.The expression levels on 2nd day and 4th day were significantly different from those on 0th day (P<0.01).From the 2nd day of differentiation,the expression increased with the adipocyte differentiation.The expression of ACTC1 gene was the highest in muscle tissues (myocardium and skeletal muscle) of cattle,and its expression characteristics were consistent with the overall trend of muscle cell differentiation.In addition,there was also a certain rule of ACTC1 gene expression in adipocytes.In summary,it was speculated that ACTC1 gene might affect the growth and development of muscle tissue and fat deposition in cattle.

Key words: ACTC1 gene; Qinchuan cattle; myoblasts; preadipocytes; Real-time quantitative PCR

CLC Number:

S823.8⁺1

LI Anqi, JIANG Lei, SU Xiaotong, ZAN Linsen, WANG Hongbao. Expression Characteristics Analysis of ACTC1 Gene in Qinchuan Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2359-2367.

References

[1] LI X Z,YAN C G,ZAN L S.Current situation and future prospects for beef production in China-A review[J].Asian-Australasian Journal of Animal Sciences,2018,31(7):984-991.
[2] ZHOU G,ZHANG W,XU X.China's meat industry revolution:Challenges and opportunities for the future[J].Meat Science,2012,92(3):188-196.
[3] 王红萍,王刚.浅谈雪花牛肉的加工与发展趋势[J].肉类工业,2013,12:49-52. WANG H P,WANG G.Discussion on processing and development trend of snowflake beef[J].Meat Industry,2013,12:49-52.(in Chinese)
[4] POURCEL L,BURON F,ARIB G,et al.Influence of cytoskeleton organization on recombinant protein expression by CHO cells[J].Biotechnol Bioeng,2020,117(4):1117-1126.
[5] ILKOVSKI B,CLEMENT S,SEWRY C,et al.Defining alpha-skeletal and alpha-cardiac actin expression in human heart and skeletal muscle explains the absence of cardiac involvement in ACTA1 nemaline myopathy[J].Neuromuscul Disord,2005,15(12):829-835.
[6] VANDEKERCKHOVE J,BUGAISKY G,BUCKINGHAM M.Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells.A quantitative determination of the two actin isoforms[J].Journal of Biological Chemistry,1986,261(4):1838-1843.
[7] MOLL R,HOLZHAUSEN H J,MENNEL H D,et al.The cardiac isoform of alpha-actin in regenerating and atrophic skeletal muscle,myopathies and rhabdomyomatous tumors:An immunohistochemical study using monoclonal antibodies[J].Virchows Archiv,2006,449(2):175-191.
[8] DENNIS R A,PRZYBYLA B,GURLEY C,et al.Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise[J].Physiological Genomics,2008,32(3):393-400.
[9] FOKSTUEN S,LYLE R,MUNOZ A,et al.A DNA resequencing array for pathogenic mutation detection in hypertrophic cardiomyopathy[J].Human Mutation,2008,29(6):879-885.
[10] YANG Q L,BIAN Y Y,WANG B,et al.Novel phenotype-genotype correlations of hypertrophic cardiomyopathy caused by mutation in alpha-actin and myosin-binding protein genes in three unrelated Chinese families[J].Journal of Cardiology,2019,73(5):438-444.
[11] AUGIERE C,MEGY S,EL MALTI R,et al.A novel alpha cardiac actin (ACTC1) mutation mapping to a domain in close contact with myosin heavy chain leads to a variety of congenital heart defects,arrhythmia and possibly midline defects[J].PLoS One,2015,10(6):e0127903.
[12] OHTAKI S,WANIBUCHI M,KATAOKA-SASAKI Y,et al.ACTC1 as an invasion and prognosis marker in glioma[J].Journal of Neurosurgery,2017,126(2):467-475.
[13] KIM E K,SONG M J,JUNG Y,et al.Proteomic ana-lysis of primary colon cancer and synchronous solitary liver metastasis[J].Cancer Genomics Proteomics,2019,16(6):583-592.
[14] KEEL B N,ZAREK C M,KEELE J W,et al.RNA-Seq meta-analysis identifies genes in skeletal muscle associated with gain and intake across a multi-season study of crossbred beef steers[J].BMC Genomics,2018,19(1):430.
[15] MEI C,JUNJVLIEKE Z,RAZA S H A,et al.Copy number variation detection in Chinese indigenous cattle by whole genome sequencing[J].Genomics,2020,112(1):831-836.
[16] LI J,LIU J,LIU S,et al.Genome-wide association study for buffalo mammary gland morphology[J].Journal of Dairy Research,2020,87(1):27-31.
[17] DU M,TONG J,ZHAO J,et al.Fetal programming of skeletal muscle development in ruminant animals[J].Journal of Animal Science,2010,88(13 Suppl):E51-E60.
[18] DU M,HUANG Y,DAS A K,et al.Meat science and muscle biology symposium:Manipulating mesenchymal progenitor cell differentiation to optimize performance and carcass value of beef cattle[J].Journal of Animal Science,2013,91(3):1419-1427.
[19] 王亚宁.MEF2A对秦川牛骨骼肌成肌细胞增殖和分化的调控作用及机理研究[D].杨凌:西北农林科技大学,2019. WANG Y N.The roles of MEF2A in the regulation of skeletal muscle myoblasts proliferation and differentiation in qinchuan beef[D].Yangling:Northwest A&F University,2019.(in Chinese)
[20] BUCKINGHAM M,RIGBY P W.Gene regulatory networks and transcriptional mechanisms that control myogenesis[J].Developmental Cell,2014,28(3):225-238.
[21] DUMONT N A,BENTZINGER C F,SINCENNES M C,et al.Satellite cells and skeletal muscle regeneration[J].Comprehensive Physiology,2015,5(3):1027-1059.
[22] ZAMMIT P S.Function of the myogenic regulatory factors Myf5,MyoD,Myogenin and MRF4 in skeletal muscle,satellite cells and regenerative myogenesis[J].Seminars in Cell & Developmental Biology,2017,72:19-32.
[23] TSAO J,VERNET D A,ROBERT G.Myostatin genetic inactivation inhibits myogenesis by muscle-derived stem cellsin vitrobut not when implanted in the mdx mouse muscle[J].Stem Cell Research & Therapy,2013,4(1):4.
[24] ROSEN E D.The transcriptional basis of adipocyte development[J].Prostaglandins Leukot Essent Fatty Acids,2005,73(1):31-34.
[25] 刘芳,陈树宝,刘晓青,等.心脏α肌动蛋白基因在胚胎心脏发育中的时空表达[J].中华儿科杂志,2005,43(10):772-776. LIU F,CHEN S B,LIU X Q,et al.Spatial expression of cardiac alpha-actin during heart development in embryos[J].Chinese Journal of Pediatrics,2005,43(10):772-776.(in Chinese)
[26] BOUTILIER J K,TAYLOR R L,RAM R,et al.Variable cardiac alpha-actin (Actc1) expression in early adult skeletal muscle correlates with promoter methylation[J].Biochimica et Biophysica Acta,2017,1860(10):1025-1036.

Expression Characteristics Analysis of ACTC1 Gene in Qinchuan Cattle

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