绵羊FGF10基因克隆、序列分析及其在毛囊发育中的表达分析

doi:10.16431/j.cnki.1671-7236.2021.02.003

Abstract

Abstract: The aim of this study was to obtain Chinese Merino sheep full-length sequence of the coding region (CDS) of fibroblast growth factor 10 (FGF10) gene,to analyze its sequence characteristics by bioinformatics method,and to study the expression characteristics of FGF10 gene.It could lay a theoretical foundation for further study of the expression and regulation mechanism of FGF10 mRNA in Chinese Merino hair follicle.The full CDS of FGF10 gene was obtained by PCR amplification and cloned into zero PCR@^TM-Blunt for sequencing verification.Real-time quantitative PCR (qRT-PCR) was used to detect the expression pattern of FGF10 gene.The results showed that the sheep FGF10 CDS was 696 bp in length (Upload the sequence to GenBank and get the accession No.:MT872422),which coded 231 amino acids,the homology of amino acid sequence with cattle and goat was 100%,there was a signal peptide and a transmembrane domain,which was the signal protein of secretory pathway.qRT-PCR analysis showed that FGF10 gene was expressed during the development of Chinese Merino follicles,and the highest expression was found on the 85th day of hair follicle development,which was significantly higher than those of other hair follicle development stages.The complete CDS and expression characteristics of FGF10 gene were obtained.Bioinformatics analysis showed that the CDS of FGF10 gene was conserved among species,and the gene was expressed in the skin tissue of different development periods of hair follicle,which indicated that FGF10 gene might play an important role in the growth and development of sheep hair follicles physical function.

Key words: Chinese Merino sheep; FGF10 gene; cloning; Real-time quantitative PCR; hair rollicle

CLC Number:

S826.8

CHEN Lei, YUAN Feng, HE Sangang, MAYILA, LI Wenrong. Cloning,Sequencing and Quantitative Expression of FGF10 Gene in Sheep During Hair Follicle Development Period[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 425-432.

References

[1] MARIA I C,SANDRA F,ELISA C,et al.A role for the TGF-β/BMP co-receptor endoglin in the molecular oscillator that regulates the hair follicle cycle[J].Journal of Molecular Cell Biology,2019,11(1):39-52.
[2] PISAL R,KAPIL D,DANIEL D,et al.Signaling involved in hair follicle morphogenesis and development[J].International Journal of Molecular Sciences,2014,15:1647-1670.
[3] XUE F L,LEI C,SAN G H,et al.Effect of nutritional restriction on the hair follicles development and skin transcriptome of Chinese Merino sheep[J].Animals,2020,10(6):2-16.
[4] HARDY M H,LYNE A G.The prenatal development of wool follicles in Merino sheep[J].The Australian Journal of Experimental Biology and Medical Science,1956,9:423-441.
[5] FRASER A S,SHORT B F.The Biology of the Fleece[M].Australia:Melbourne,CSIRO,1960.
[6] ITOH N.FGFs as multifunctional signaling molecules:Diversity of strurcture and function[J].Seikagaku,2001,73(7):525-535.
[7] HAMADA K,OZAWA K,ITAMI S,et al.Human fibroblast growth factor 10 expression in dermal papilla cells,outer root sheath cells and keratinocytes[J].Experimental Dermatology,1999,8(4):347-349.
[8] SUZUKI K,YAMANISHI K,MORI O,et al.Defective terminal differentiation and hypoplasia of the epidermis in mice lacking the FGF10 gene[J].FEBS Letters,2000,481(1):53-56.
[9] YUE Z C,JIANG T X,WU P,et al.Sprouty/FGF signaling regulates the proximal-distal feather morphology and the size of dermal papillae[J].Developmental Biology,2012,372(1):45-54.
[10] THOMAS S.FGF signals specifically regulate the structure of hair shaft medulla via IGF-binding protein 5[J].Development,2005,132(13):2981-2990.
[11] ZHANG H H,NAN W X,WANG S Y,et al.Balance between fibroblast growth factor 10 and secreted frizzled-relate protein-1 controls the development of hair follicle by competitively regulating β-catenin signaling[J].Biomedicine Pharmacotherapy,2018,103:1531-1537.
[12] HIDEYO O,HIROTAKA T,KAZUYO O,et al.Fibroblast growth factor 10 is required for proper development of the mouse whiskers[J].Biochemical and Biophysical Research Communications,2003,302(3):562-567.
[13] SONG H,WANG Y,GOETINCK P F.Fibroblast growth factor 2 can replace ectodermal signaling for feather development[J].Proceedings of the National Academy of Sciences of the United States of America,1996,93(19):10246-10249.
[14] LIN W H,XIANG L J,SHI H X,et al.Fibroblast growth factors stimulate hair growth through β-catenin and SHH expression in C57BL/6 mice[J].Biomed Research International,2015,5:730.
[15] HUANG J,LISA K D,RAMYA R,et al.FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate[J].Development,2009,36(10):1741-1750.
[16] MORIA M,SALLY S,ALAN B,et al.Gene expression profiles of BMP4,FGF10 and cognate inhibitors,in the skin of Foetal Merino sheep,at the time of secondary follicle branching[J].Experimental Dermatology,2009,18(10):877-879.
[17] ANDREI N M,MOHAMMED I A,NIKOLA V V,et al.Micro-RNA-31 controls hair cycle-associated changes in gene expression programs of the skin and hair follicle[J].FASEB Journal,2010,24(10):3869-3881.
[18] 李倩,林亚秋,朱江江,等.山羊FGF10基因的克隆及其表达分析[J].农业生物技术学报,2016,24(11):1726-1733. LI Q,LIN Y Q,ZHU J J,et al.Cloning and expression analysis of capra hircus FGF10 gene[J].Journal of Agricultural Biotechnology,2016,24(11):1726-1733.(in Chinese)
[19] SAHARA S,LEARY D M.FGF10 regulates transition period of cortical stem cell differentiation to radial glia controlling generation of neurons and basal progenitors[J].Neuron,2009,63(1):48-62.
[20] TAGASHIRA S,HARADA H,KATSUMATA T,et al.Cloning of mouse FGF10 and upregulation of its gene expression during wound healing[J].Gene,1997,197(1-2):399-404.
[21] PIETRO G,SIMONE G.Advances in regenerative stem cell therapy in androgenic alopecia and hair loss:Wnt pathway,growth-factor,and mesenchymal stem cell signaling impact analysis on cell growth and hair follicle development[J].Cells,2019,8(5):466.
[22] KIYOON K,WONCHAE C.Recombinant human cyclophilin A stimulates hair follicle cells via Wnt/β-catenin signaling pathway[J].Biotechnology Letters,201941(12):1451-1458.
[23] SANDRA F M,MARIA C S,KARLA G A,et al.Sustained human hair follicle growth ex vivo in a glycosaminoglycan hydrogel matrix[J].International Journal of Molecular Science,2019,20(7):1741.

Cloning,Sequencing and Quantitative Expression of FGF10 Gene in Sheep During Hair Follicle Development Period

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