秦川牛Snail2基因扩增、序列特征及表达特性分析

doi:10.16431/j.cnki.1671-7236.2021.09.005

Abstract

Abstract: The purpose of this experiment was to amplify the Snail2 gene of Qinchuan cattle, and construct the temporal and spatial expression pattern of Snail2 gene in different developmental stages of tissues and different differentiation stages of adipocytes, which laid a foundation for further study on the function and regulation of Snail2 gene in bovine fat deposition. Qinchuan cattle were taken as the research object, the CDS region sequence of Snail2 gene was amplified by PCR, and its functional structure was predicted by bioinformatics softwares. At the same time, Real-time quantitative PCR was used to detect the temporal and spatial expression profile of Snail2 gene in the process of adipogenic differentiation of newborn and adult bovine tissues and adipocytes. The results showed that the full length of Snail2 gene coding sequence of Qinchuan cattle was 952 bp. The phylogenetic tree results of different species showed that bovine Snail2 protein was highly conserved in cattle, buffalo, goat and sheep. 41 potential phosphorylation sites were found by phosphorylation site analysis, in which cyclin dependent kinase 1 (CDK1), CDK5, CKⅡ, CKⅠ and other cell cycle-related kinases were involved in the phosphorylation of Snail2 protein. Protein domain prediction found that there were 8 similar Motif among 8 species including cattle, human and mouse, etc. The secondary structure of Snail2 protein was mainly composed of irregular curls, and the prediction results of secondary and tertiary structures were consistent. Sequence analysis of the promoter region of Snail2 gene revealed a CpG island and E2F, C/EBPα(CCAAT/enhancer binding proteins alpha), AP2 and Sp1 transcription factor binding sites related to adipogenesis. The results of Real-time quantitative PCR showed that Snail2 gene was highly expressed in bovine adipose tissue, and showed an upward trend with adipogenic differentiation and bovine growth and development, indicating that Snail2 gene played an important role in bovine fat deposition. The results laid a foundation for further revealing the mechanism of Snail2 gene affecting fat deposition in beef cattle by affecting the proliferation and differentiation of adipocytes.

Key words: Qinchuan cattle; Snail2 gene; bioinformatics; temporal and spatial expression pattern

CLC Number:

S823

TIAN Yuan, LONG Feng, LI Anqi, CHEN Jiayue, YANG Sen, ZAN Linsen, CHENG Gong. Amplification, Sequence Feature and Expression Pattern of Snail2 Gene in Qinchuan Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3147-3157.

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Amplification, Sequence Feature and Expression Pattern of Snail2 Gene in Qinchuan Cattle

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