绵羊PDGFD基因克隆及其在不同部位脂肪组织中的表达

doi:10.16431/j.cnki.1671-7236.2021.09.004

Abstract

Abstract: The aim of this experiment was to obtain the full-length coding sequence (CDS) of PDGFD gene in sheep, and understand its expression in adipose tissue deposited in different parts of the body. The full-length CDS sequence of PDGFD gene was cloned from Altay ewes and Chinese Merino ewes, and the bioinformatics analysis of the sequence was carried out. The expression of PDGFD gene in adipose tissues of the tail, mesentery, back and kidney of the two breeds of sheep was quantitatively analyzed by Real-time quantitative PCR. The results showed that the total CDS region sequence of PDGFD gene was 1 113 bp, which encoded 370 amino acids. The sequence of CDS region and its coding sequence of PDGFD gene had the highest similarity with Capra hircus, followed by Bos taurus, Sus scrofa, Equus caballus and Homo sapiens, and the lowest similarity with Mus musculus, which was consistent with the result of phylogenetic tree analysis. It was predicted that PDGFD protein was a stable hydrophilic protein, no transmembrane structure, had signal peptide sequence. PDGFD protein contained a glycosylation site, a CUB domain and a PDGF domain, and the secondary structure of PDGFD protein were composed of alpha helix, beta turn, extended chain and random coil, and tertiary structure was basically consistent with the analysis results of domain and secondary structure. Real-time quantitative PCR results showed that the expression of PDGFD gene in four adipose tissues of Altay sheep was higher than that of Chinese Merino sheep, especially the expression in tail fat and back fat of Altay sheep was significantly higher than of Chinese Merino sheep (P<0.05). The results of this study provided reference for further research on the mechanism of PDGFD gene in adipose deposition in sheep.

Key words: sheep; PDGFD gene; gene cloning; adipose

CLC Number:

S826.9⁺2

LIU Jinrui, LI Zhonghui, JIANG Fangfang, MA Yila, LI Wenrong, LI Haiying. Cloning of PDGFD Gene and Its Expression in Different Adipose Tissue in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3138-3146.

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Cloning of PDGFD Gene and Its Expression in Different Adipose Tissue in Sheep

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