葛根素对松辽黑猪前体脂肪细胞成脂分化的影响

doi:10.16431/j.cnki.1671-7236.2021.12.010

Abstract

Abstract: In order to investigate the regulation effect of puerarin on the adipogenic differentiation of precursor adipocytes in Songliao Black pig, 0, 10, 20, 40, 60 and 80 μmol/L puerarin were added into the cell induction solution to induce adipogenic differentiation, respectively.In order to determine the effect of lipid deposition and differentiation, oil red O staining and triglyceride assay were used to detect the accumulation of lipid droplets and triglyceride content during adipocyte differentiation, and to determine the best concentration of puerarin.The mRNA expression levels of lipid marker genes cell peroxisome proliferator-activated receptor γ(PPARγ), CCAAT-enhancer binding protein α(C/EBPα) and genes related to adipogenic differentiation like acetyl-CoA carboxylase (ACC), fatty acid binding protein(FABP4), stress protein(TRIB) and forked frame protein O1 (FOXO1) were detected by Real-time quantitative PCR in the control group and the optimal puerarin concentration group.The protein expression levels of PPARγ and C/EBPα were detected by Western blotting.The results showed that compared with control group, 20, 40 and 60 μmol/L puerarin significantly increased the content of lipid droplets and triglycerides (P<0.05), and 40 μmol/L puerarin had the best effect.Real-time quantitative PCR results showed that compared with control group, 40 μmol/L puerarin significantly up-regulated the expression of adipogenic marker genes PPARγ and C/EBPα and adipogenic differentiation genes ACC, FABP4, FOXO1 and TRIB (P<0.05).Western blotting results showed that compared with control group, 40 μmol/L puerarin significantly increased the expression of PPARγ protein (P<0.05).In conclusion, 40 μmol/L puerarin could promote the adipogenic differentiation and lipid deposition of preadipocytes in Songliao Black pigs.

Key words: puerarin; Songliao Black pig; adipogenic differentiation; adipocyte

CLC Number:

S828

LYU Xiangzhou, WEI Minghong, WU Sihui, LUO Xiaotong, ZHANG Qi, JIN Yi, YU Yongsheng. Effect of Puerarin on Adipogenic Differentiation of Preadipocytes in Songliao Black Pig[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4422-4428.

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Effect of Puerarin on Adipogenic Differentiation of Preadipocytes in Songliao Black Pig

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