China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (10): 3232-3241.doi: 10.16431/j.cnki.1671-7236.2020.10.022

• Genetics and Breeding • Previous Articles     Next Articles

Genetic Diversity Analysis of Three Newly Discovered Native Chicken Breeds in Yunnan Using mtDNA D-loop Region Sequences

WANG Kun, DOU Tengfei, LI Qihua, LIU Mingzheng, LI Fengyun, JIA Junjing, GE Changrong   

  1. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
  • Received:2020-03-28 Online:2020-10-20 Published:2020-10-17

Abstract: This study was aimed to evaluate the information of their genetic background of Frizzle chicken (FM),Naked-neck chicken (CB) and YN chicken (YN) that were three newly discovered native chicken genetic resources with excellent characteristics,which had been found in Nujiang prefecture and mountainous area of Yunnan province.The variation in a total of 168 individuals sampled from the three chicken populations was assessed using the mitochondrial DNA (mtDNA) D-loop region sequences as genetic marker.The results showed that there were a total of 27 haplotypes were defined in the three native chicken as well as the haplotype diversity of these three chicken were 0.947,0.938 and 0.596,respectively.The nucleotide diversity of these three chicken were 0.01268,0.01434 and 0.00239,respectively.Phylogenetic tree displayed that all 168 individuals distributed in maternal lineage A,B,C,E,F and G.Frizzle chicken contained lineages E,F and G,and Naked-neck chicken contained all 6 lineages,of which the main lineages were E,F and G.YN chicken included lineages E,F and G,and lineage E was the highest percentage in this population.Also,it found that YN chicken was closely related to Gallus gallus murghi,White Plymouth Rock chicken,White Leghorn chicken and New Hampshires chicken,while Naked-neck chicken was closely related to Gallus gallus spadiceus,Gallus gallus jabouillei,Gallus gallus gallus Linnaeus,Indonesian cockfight and Laos chicken.The Frizzle chickens shared more haplotypes with Naked-neck chicken,and the evolutionary relationship between the two species was closer.This study provided a basis for the origin and genetic assessment of three newly discovered Yunnan local chicken breeds.

Key words: Frizzle chicken; Naked-neck chicken; YN chicken; mitochondrial DNA D-loop region; genetic diversity

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