基于线粒体Cytb基因的贵州野猪(Sus scrofa) 群体遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2022.07.018

Abstract

Abstract: 【Objective】 The objective of this study was to evaluate the genetic diversity and genetic structure of Sus scrofa in Guizhou,so as to provide theoretical basis for further conservation genetics research and population ecological management,and then lay a foundation of the research of Sus scrofa conservation biology and its adaptation to the environment.【Method】 The mitochondrial Cytb gene of Sus scrofa was amplified by PCR and sequenced.Combined with 55 mitochondrial Cytb gene sequences of Sus scrofa downloaded from GenBank in other 9 regions in China,Mega 7.0 software was used for base composition and genetic distance analysis,and DNAsp6 software was used for genetic diversity analysis.【Result】 The ratio of Cytb gene (1 140 bp) of Sus scrofa in Guizhou was similar with other region,and AT content was higher than GC content.The 11 sequences had 6 variation sites and 4 haplotypes.The haplotype diversity was 0.600,and the nucleotide diversity was 0.00118.Haplotype diversity and nucleotide diversity were only higher than those of Sus scrofa populations in Jiangxi and Northeastern region.The genetic distance within species was small,and the genetic relationship was close.The neutral test Tajima's D and Fu's Fs values were negative,and the difference was not significant.The mismatch distribution analysis showed a unimodal distribution,it showed that Sus scrofa population in Guizhou was relatwely stable in history.The haplotype diagram was constructed using Network,and the analysis of haplotype phylogenetic tree constructed by Mega 7.0,which showed the haplotypes of Sus scrofa in Guizhou were shared with populations reported in other region (Hap_1 and Hap_10).【Conclusion】 This study concluded that Sus scrofa population in Guizhou was experiencing an expansion phase,but the population genetic diversity was lower than the populations in other region in China,the genetic distance of intrapopulation was small,and moderate comparing with the populations from other region in China.Therefore,the conservation management on genetic biodiversity of Sus scrofa in Guizhou should be paid more attention.

Key words: Sus scrofa; mitochondrial genome; Cytb gene; genetic diversity; phylogenetic

CLC Number:

S813.3

PENG Caichun, LI Binqiang, WANG Yeying, SU Haijun. Genetic Diversity Analysis of Sus scrofa Population in Guizhou Based on Mitochondrial CytbGene[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2601-2612.

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Genetic Diversity Analysis of Sus scrofa Population in Guizhou Based on Mitochondrial CytbGene

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